Fisetin

Catalog No.S2298 Batch:S229809

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Technical Data

Formula

C15H10O6

Molecular Weight 286.24 CAS No. 528-48-3
Solubility (25°C)* In vitro DMSO 57 mg/mL (199.13 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Fisetin (Fustel) is a potent sirtuin activating compound (STAC) and an agent that modulates sirtuins.
Targets
Sirtuin [1]
In vitro

Fisetin selectively kills SPiDER-β-Gal positive senescence neural cells in vitro.[2]

In vivo

Oral administration of fisetin, a senolytic drug, reduces the number of senescent neural cells observed, the SASP expression level, and depressive behavior in MRL/lpr mice.[2]

Protocol (from reference)

Cell Assay:

[2]

  • Cell lines

    Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells

  • Concentrations

    5, 10, or 20 μM

  • Incubation Time

    48 h

  • Method

    Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells are seeded on a 96-well black/clear bottom plate at 40,000 cells and 10,000 cells per well, respectively. After senescence induction for 6 days, 5 μM, 10 μM, or 20 μM fisetin is added and the cells are incubated for 48 h. Cell number and cellular senescence are determined by DAPI staining and SPiDER-β-Gal staining, respectively. Cells are washed twice with PBS, fixed in 4% paraformaldehyde at room temperature for 5 min, and washed twice with PBS. Sections are incubated in 20 M SPiDER-β-gal in solution in McIlvaine buffer (pH 6.0) for 60 min at 37 ℃. After washing of tissue sections, nuclei are stained with DAPI. Cells are observed. 

Animal Study:

[2]

  • Animal Models

    Female MRL/lpr mice, haplotype-matched female MRL/MPJ mice

  • Dosages

    100 mg/kg

  • Administration

    p.o.

Customer Product Validation

Data from [Data independently produced by , , Alcohol Clin Exp Res, 2016, 40(10):2076-2084.]

Selleck's Fisetin has been cited by 40 publications

TAGLN2 induces resistance signature ISGs by activating AKT-YBX1 signal with dual pathways and mediates the IFN-related DNA damage resistance in gastric cancer [ Cell Death Dis, 2024, 15(8):608] PubMed: 39168971
Senolytic intervention improves cognition, metabolism, and adiposity in female APPNL-F/NL-F mice [ Geroscience, 2024, 10.1007/s11357-024-01308-8] PubMed: 39120687
Single-cell morphology encodes functional subtypes of senescence in aging human dermal fibroblasts [ bioRxiv, 2024, 2024.05.10.593637] PubMed: 38798365
Clearance of defective muscle stem cells by senolytics restores myogenesis in myotonic dystrophy type 1 [ Nat Commun, 2023, 14(1):4033] PubMed: 37468473
Fisetin ameliorates fibrotic kidney disease in mice via inhibiting ACSL4-mediated tubular ferroptosis [ Acta Pharmacol Sin, 2023, 10.1038/s41401-023-01156-w] PubMed: 37696989
Tubular cell senescence promotes maladaptive kidney repair and chronic kidney disease after cisplatin nephrotoxicity [ JCI Insight, 2023, 8(8)e166643] PubMed: 36917180
Post-translational modification of CDK1-STAT3 signaling by fisetin suppresses pancreatic cancer stem cell properties [ Cell Biosci, 2023, 13(1):176] PubMed: 37743465
PPARγ activation by fisetin mitigates vascular smooth muscle cell senescence via the mTORC2-FoxO3a-autophagy signaling pathway [ Biochem Pharmacol, 2023, S0006-2952(23)00485-9] PubMed: 37890594
Sexual dimorphic metabolic and cognitive responses of C57BL/6 mice to Fisetin or Dasatinib and quercetin cocktail oral treatment [ Geroscience, 2023, 45(5):2835-2850] PubMed: 37296266
Fisetin, a Natural Polyphenol, Ameliorates Endometriosis Modulating Mast Cells Derived NLRP-3 Inflammasome Pathway and Oxidative Stress [ Int J Mol Sci, 2023, 24(6)5076] PubMed: 36982152

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.