Home TGF-beta/Smad TGF-beta/Smad agonist SB 4 For research use only.

SB 4

SB 4 is a potent and selective agonist of bone morphogenetic protein 4 (BMP4) signaling with EC50 of 74 nM. SB 4 enhances canonical BMP signaling and activates SMAD-1/5/9 phosphorylation.

SB 4 Chemical Structure

SB 4 Chemical Structure

CAS No. 100874-08-6

Purity & Quality Control

SB 4 Related Products

Signaling Pathway

TGF-beta/Smad Signaling Pathways

TGF-beta/Smad Signaling Pathway

Biological Activity

Description SB 4 is a potent and selective agonist of bone morphogenetic protein 4 (BMP4) signaling with EC50 of 74 nM. SB 4 enhances canonical BMP signaling and activates SMAD-1/5/9 phosphorylation.
Targets
BMP4 [1]
(Cell-free assay)
74 nM(EC50)
In vitro
In vitro

BMP signaling agonist SB 4 induces phosphorylated SMAD-1/5/9 in a dose-dependent manner in PRECs cells under serum-starved conitions. SB 4 enhances the efficacy of signaling at each concentration of rhBMP4 tested. SB 4 increases BRE-luc expression 2-fold at 0.4 ng/ml of rhBMP4.[1]
Cell Research Cell lines Primary mouse kidney epithelial cells (PRECs)
Concentrations 0.05 μM-1 μM
Incubation Time 0-1 h, 24 h
Method

The BRE-Luc cells are stimulated with 2 ng/ml rhBMP4 for 1 h, after which the media are removed and replaced with fresh media containing either DMSO or 1 μm SB 4. p-SMAD-1/5/9 levels are then examined after 0, 5, 15, 30, 45, and 60 min of SB 4 treatment and compared with DMSO controls.
Experimental Result Images Methods Biomarkers Images PMID
Western blot p-SMAD-1/5/9 / t-SMAD-1 / β-Actin p-SMAD-1/5/9 / t-SMAD-1 / β-Actin 30602563

Chemical Information & Solubility

Molecular Weight 320.20 Formula

C14H10BrNOS
CAS No. 100874-08-6 SDF --
Smiles C1=CC=C2C(=C1)N=C(O2)SCC3=CC=C(C=C3)Br
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 64 mg/mL ( (199.87 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 21 mg/mL

Water : ˂1 mg/mL


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In vivo
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