SB 4

Catalog No.S0523 Batch:S052302

Technical Data

Formula

C₁₄H₁₀BrNOS

Molecular Weight

320.20

CAS No.

100874-08-6

Solubility (25°C)*

In vitro

DMSO

64 mg/mL (199.87 mM)

Ethanol

21 mg/mL (65.58 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	3.2mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 64 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	3.2mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 64 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

SB 4 is a potent and selective agonist of bone morphogenetic protein 4 (BMP4) signaling with EC50 of 74 nM. SB 4 enhances canonical BMP signaling and activates SMAD-1/5/9 phosphorylation.

Targets

BMP4 ^[1]
(Cell-free assay)

74 nM(EC50)

In vitro

BMP signaling agonist SB 4 induces phosphorylated SMAD-1/5/9 in a dose-dependent manner in PRECs cells under serum-starved conitions. SB 4 enhances the efficacy of signaling at each concentration of rhBMP4 tested. SB 4 increases BRE-luc expression 2-fold at 0.4 ng/ml of rhBMP4.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Primary mouse kidney epithelial cells (PRECs) Concentrations 0.05 μM-1 μM Incubation Time 0-1 h, 24 h Method The BRE-Luc cells are stimulated with 2 ng/ml rhBMP4 for 1 h, after which the media are removed and replaced with fresh media containing either DMSO or 1 μm SB 4. p-SMAD-1/5/9 levels are then examined after 0, 5, 15, 30, 45, and 60 min of SB 4 treatment and compared with DMSO controls.

Cell Assay:^{^[1]}

Cell lines
Primary mouse kidney epithelial cells (PRECs)
Concentrations
0.05 μM-1 μM
Incubation Time
0-1 h, 24 h
Method
The BRE-Luc cells are stimulated with 2 ng/ml rhBMP4 for 1 h, after which the media are removed and replaced with fresh media containing either DMSO or 1 μm SB 4. p-SMAD-1/5/9 levels are then examined after 0, 5, 15, 30, 45, and 60 min of SB 4 treatment and compared with DMSO controls.

References

[1] Bradford STJ, Ranghini EJ, Grimley E, Lee PH, Dressler GR. High-throughput screens for agonists of bone morphogenetic protein (BMP) signaling identify potent benzoxazole compounds. J Biol Chem. 2019 Mar 1;294(9):3125-3136.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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