Home TGF-beta/Smad TGF-beta/Smad activator Kartogenin For research use only.

Kartogenin

Synonyms: KGN

Kartogenin (KGN) is an activator of the smad4/smad5 pathway, and promotes the selective differentiation of multipotent mesenchymal stem cells into chondrocytes.

Kartogenin Chemical Structure

Kartogenin Chemical Structure

CAS No. 4727-31-5

Purity & Quality Control

Kartogenin Related Products

Signaling Pathway

TGF-beta/Smad Signaling Pathways

TGF-beta/Smad Signaling Pathway

Biological Activity

Description Kartogenin (KGN) is an activator of the smad4/smad5 pathway, and promotes the selective differentiation of multipotent mesenchymal stem cells into chondrocytes.
Targets
Smad4 [1] Smad5 [1]
In vitro
In vitro Kartogenin induces chondrocyte differentiation from hMSCs by enhancing the nuclear localization of CBFβ, and protects articular chondrocytes. [1] Kartogenin promotes the type-I collagen synthesis of dermal fibroblasts via activation of the smad4/smad5 pathway without obvious cytotoxicity on fibroblasts’ viability, morphology and survival. [2]
Cell Research Cell lines Fibroblasts
Concentrations ~1 μM
Incubation Time 48 hours
Method For the cell viability assay, fibroblasts are seeded on six-well plates, followed by Kartogenin or DMSO treatment. According to the manufacturer’s instructions, cell viability and viability are assessed by AlamarBlue assay.
In Vivo
In vivo Kartogenin promotes cartilage repair in collagenase VII (10 μM)– and surgery (100 μM)-induced OA models in mice. [1] Kartogenin (100 nM) also stimulats collagen synthesis in the mouse dermis. [2] In addition, Kartogenin stimulates mouse limb skeletal growth and synovial joint development. [3]
Animal Research Animal Models Collagenase VII-induced OA mouse model and Surgery-induced OA mouse model
Dosages 10 μM or 100 μM
Administration Intra-articular injection

Chemical Information & Solubility

Molecular Weight 317.34 Formula

C20H15NO3
CAS No. 4727-31-5 SDF Download Kartogenin SDF
Smiles C1=CC=C(C=C1)C2=CC=C(C=C2)NC(=O)C3=CC=CC=C3C(=O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 63 mg/mL ( (198.52 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 18 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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