Kartogenin

Catalog No.S7658 Batch:S765802

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Technical Data

Formula

C20H15NO3
Molecular Weight 317.34 CAS No. 4727-31-5
Solubility (25°C)* In vitro DMSO 63 mg/mL (198.52 mM)
Ethanol 5 mg/mL (15.75 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Kartogenin (KGN) is an activator of the smad4/smad5 pathway, and promotes the selective differentiation of multipotent mesenchymal stem cells into chondrocytes.
Targets
Smad4 [1] Smad5 [1]
In vitro Kartogenin induces chondrocyte differentiation from hMSCs by enhancing the nuclear localization of CBFβ, and protects articular chondrocytes. [1] Kartogenin promotes the type-I collagen synthesis of dermal fibroblasts via activation of the smad4/smad5 pathway without obvious cytotoxicity on fibroblasts’ viability, morphology and survival. [2]
In vivo Kartogenin promotes cartilage repair in collagenase VII (10 μM)– and surgery (100 μM)-induced OA models in mice. [1] Kartogenin (100 nM) also stimulats collagen synthesis in the mouse dermis. [2] In addition, Kartogenin stimulates mouse limb skeletal growth and synovial joint development. [3]

Protocol (from reference)

Cell Assay:[2]
  • Cell lines

    Fibroblasts

  • Concentrations

    ~1 μM

  • Incubation Time

    48 hours

  • Method

    For the cell viability assay, fibroblasts are seeded on six-well plates, followed by Kartogenin or DMSO treatment. According to the manufacturer’s instructions, cell viability and viability are assessed by AlamarBlue assay.
Animal Study:[1]
  • Animal Models

    Collagenase VII-induced OA mouse model and Surgery-induced OA mouse model

  • Dosages

    10 μM or 100 μM

  • Administration

    Intra-articular injection

Selleck's Kartogenin has been cited by 10 publications

Enhanced chondrogenic potential in GelMA-based 3D cartilage model via Wnt3a surface immobilization [ Sci Rep, 2024, 14(1):15022] PubMed: 38951570
Fabrication of Injectable Kartogenin-Conjugated Composite Hydrogel with a Sustained Drug Release for Cartilage Repair [ Pharmaceutics, 2023, 15(7)1949] PubMed: 37514135
An Injectable Hydrogel Scaffold Loaded with Dual-Drug/Sustained-Release PLGA Microspheres for the Regulation of Macrophage Polarization in the Treatment of Intervertebral Disc Degeneration [ Int J Mol Sci, 2022, 24(1)390] PubMed: 36613833
Construction of Biocompatible Hydrogel Scaffolds With a Long-Term Drug Release for Facilitating Cartilage Repair [ Front Pharmacol, 2022, 13:922032] PubMed: 35784682
Kartogenin Improves Osteogenesis of Bone Marrow Mesenchymal Stem Cells via Autophagy [ Stem Cells Int, 2022, 2022:1278921] PubMed: 36591373
A chondrogenesis induction system based on a functionalized hyaluronic acid hydrogel sequentially promoting hMSC proliferation, condensation, differentiation, and matrix deposition [ Acta Biomater, 2021, S1742-7061(21)00015-5] PubMed: 33444801
Kartogenin Inhibits Prostate Cancer Cell Growth Through Smad2 Activation and Decreases Androgen Receptor Nuclear Localization [ Anticancer Res, 2021, 41(10):4753-4759] PubMed: 34593424
Intra-articular Injection of Kartogenin-Incorporated Thermogel Enhancing Osteoarthritis Treatment. [ Front Chem, 2019, 7:677] PubMed: 31681730
Effects of kartogenin on the attenuated nucleus pulposus cell degeneration of intervertebral discs induced by interleukin-1β and tumor necrosis factor-α [ Int J Mol Med, 2018, 41(2):749-756] PubMed: 29207013
Retinoic acid and TGF-β signalling cooperate to overcome MYCN-induced retinoid resistance. [Duffy DJ, et al. Genome Med, 2017, 10.1186/s13073-017-0407-3] PubMed: 28187790

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.