Home TGF-beta/Smad TGF-beta/Smad agonist SRI-011381 (C381) For research use only.

SRI-011381 (C381)

Synonyms: C381

SRI-011381 (C381) is a novel agonist of the TGF-beta signaling pathway for treatment of Alzheimer's disease.C381 physically targets the lysosome, promotes lysosomal acidification, increases breakdown of lysosomal cargo, and improves lysosome resilience to damage.

SRI-011381 (C381) Chemical Structure

SRI-011381 (C381) Chemical Structure

CAS No. 1629138-41-5

Purity & Quality Control

SRI-011381 (C381) Related Products

Signaling Pathway

TGF-beta/Smad Signaling Pathways

TGF-beta/Smad Signaling Pathway

Biological Activity

Description SRI-011381 (C381) is a novel agonist of the TGF-beta signaling pathway for treatment of Alzheimer's disease.C381 physically targets the lysosome, promotes lysosomal acidification, increases breakdown of lysosomal cargo, and improves lysosome resilience to damage.
Targets
TGF-β [1]
In vitro
In vitro

SRI-011381 partially reverses the effect of sodium ferulate (SF) on the expression of fibrosis-related proteins, by increasing TGF-β1, NALP3, collagen-1, and α-SMA expression.[2]
Cell Research Cell lines pulmonary fibroblasts of silicosis mouse model
Concentrations 10 µM
Incubation Time --
Method

The tissues are extracted in a sterile environment, rinsed in PBS 3 times, and then cut into 1-mm3 tissue blocks. After repeated digestion with 0.25% trypsin at 37 ℃, the histolysate is evenly spread in a 10-cm petri dish. A high-glucose medium containing 10% fetal bovine serum, penicillin, and streptomycin is added to the petri dish. The cells are cultured in an incubator, and the medium is exchanged every 2-3 days. When the cells reached 80-90% confluence, they are passaged according to the ratio of 1: 3, and the fifth passage cells are taken for experimental study. Pulmonary fibroblasts are identified using anti-vimentin antibody with fluorescent secondary antibodies under a fluorescence microscope. For in vitro experiments, a range of concentrations of SF solution (0.04, 0.1, 0.18 and 0.28 mg/mL) are prepared for the treatment of fibroblasts. A 10 μM SRI-011381 solution is used for the activation of TGF-β1 signaling.
In Vivo
In vivo

SRI-011381, a small-molecule agonist of TGFβ signaling, has no effect on outflow tract (OFT) expansion in zebrafish, suggesting that hyperactivation of TGFβ signaling is not sufficient to induce aneurysm.[3]
Animal Research Animal Models Zebrafish carrying ltbp3fb28 and ltbp1fb29
Dosages 500 µM
Administration exposed

Chemical Information & Solubility

Molecular Weight 329.48 Formula
C20H31N3O
 
CAS No. 1629138-41-5 SDF --
Smiles C1CCC(CC1)NC(=O)N(CC2CCNCC2)CC3=CC=CC=C3
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 66 mg/mL ( (200.31 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 66 mg/mL

Water : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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