SB 4

Catalog No.S0523 Batch:S052301

Technical Data

Formula	C₁₄H₁₀BrNOS
Molecular Weight	320.20	CAS No.	100874-08-6
Solubility (25°C)*	In vitro	DMSO	64 mg/mL (199.87 mM)
		Ethanol	21 mg/mL (65.58 mM)
		Water	˂1 mg/mL
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

SB 4 is a potent and selective agonist of bone morphogenetic protein 4 (BMP4) signaling with EC50 of 74 nM. SB 4 enhances canonical BMP signaling and activates SMAD-1/5/9 phosphorylation.

Targets

BMP4 ^[1]
(Cell-free assay)

74 nM(EC50)

In vitro

BMP signaling agonist SB 4 induces phosphorylated SMAD-1/5/9 in a dose-dependent manner in PRECs cells under serum-starved conitions. SB 4 enhances the efficacy of signaling at each concentration of rhBMP4 tested. SB 4 increases BRE-luc expression 2-fold at 0.4 ng/ml of rhBMP4.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Primary mouse kidney epithelial cells (PRECs) Concentrations 0.05 μM-1 μM Incubation Time 0-1 h, 24 h Method The BRE-Luc cells are stimulated with 2 ng/ml rhBMP4 for 1 h, after which the media are removed and replaced with fresh media containing either DMSO or 1 μm SB 4. p-SMAD-1/5/9 levels are then examined after 0, 5, 15, 30, 45, and 60 min of SB 4 treatment and compared with DMSO controls.

Cell Assay:^{^[1]}

Cell lines
Primary mouse kidney epithelial cells (PRECs)
Concentrations
0.05 μM-1 μM
Incubation Time
0-1 h, 24 h
Method
The BRE-Luc cells are stimulated with 2 ng/ml rhBMP4 for 1 h, after which the media are removed and replaced with fresh media containing either DMSO or 1 μm SB 4. p-SMAD-1/5/9 levels are then examined after 0, 5, 15, 30, 45, and 60 min of SB 4 treatment and compared with DMSO controls.

References

[1] Bradford STJ, Ranghini EJ, Grimley E, Lee PH, Dressler GR. High-throughput screens for agonists of bone morphogenetic protein (BMP) signaling identify potent benzoxazole compounds. J Biol Chem. 2019 Mar 1;294(9):3125-3136.

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SHIPPING AND STORAGE
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