Home Cell Cycle CDK inhibitor - Apoptosis related activator NVP-2 For research use only.

NVP-2

NVP-2, a potent, selective, non-neurotoxic and ATP-competitive cyclin dependent kinase 9 (CDK9) inhibitor with IC50 of 0.514 nM for CDK9/CycT activity and induces cell apoptosis.

NVP-2 Chemical Structure

NVP-2 Chemical Structure

CAS No. 1263373-43-8

Purity & Quality Control

NVP-2 Related Products

Signaling Pathway

CDK Signaling Pathways

CDK Signaling Pathway

Biological Activity

Description NVP-2, a potent, selective, non-neurotoxic and ATP-competitive cyclin dependent kinase 9 (CDK9) inhibitor with IC50 of 0.514 nM for CDK9/CycT activity and induces cell apoptosis.
Targets
CDK9/CycT [1]
(Cell-free assay)
0.514 nM
In vitro
In vitro

NVP-2 selectively inhibits CDK9. NVP-2 exhibits anti-proliferative effects and transcription inhibition. NVP-2 diminishes elongating polymerase II.[1]
Cell Research Cell lines Wildtype MOLT4 Cells, CRBN−/− MOLT4 Cells
Concentrations 1μM, 500nM, 250nM
Incubation Time 6 h, 72 h
Method

Proliferation assays are performed by treating MOLT4 and CRBN−/− MOLT4 cells with NVP-2 at concentrations indicated for 72 hours. Anti-proliferative effects of NVP-2 are assessed using Cell Titer Glo assay kit. IC50s are determined using Graphpad Prism non-linear regression curve fit. To determine the effect of washout, MOLT4 and CRBN−/− MOLT4 cells are treated with 250 nM NVP-2 or DMSO. After 6 hours, compound is washed from cells by washing cells with media three times where indicated. Cells are allowed to grow for another 66 hours (72-hour total) and assayed for anti-proliferation using Cell Titer Glo assay kit as above.

Chemical Information & Solubility

Molecular Weight 513.07 Formula

C27H37ClN6O2
CAS No. 1263373-43-8 SDF --
Smiles CC(COC)NC1CCC(CC1)NC2=NC=C(C(=C2)C3=NC(=CC=C3)NCC4(CCOCC4)C#N)Cl
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (194.9 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 100 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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