NVP-2

Catalog No.S8981 Batch:S898101

Technical Data

Formula	C₂₇H₃₇ClN₆O₂
Molecular Weight	513.07	CAS No.	1263373-43-8
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (194.9 mM)
		Ethanol	100 mg/mL (194.9 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

NVP-2, a potent, selective, non-neurotoxic and ATP-competitive cyclin dependent kinase 9 (CDK9) inhibitor with IC50 of 0.514 nM for CDK9/CycT activity and induces cell apoptosis.

Targets

CDK9/CycT ^[1]
(Cell-free assay)

0.514 nM

In vitro

NVP-2 selectively inhibits CDK9. NVP-2 exhibits anti-proliferative effects and transcription inhibition. NVP-2 diminishes elongating polymerase II.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Wildtype MOLT4 Cells, CRBN−/− MOLT4 Cells Concentrations 1μM, 500nM, 250nM Incubation Time 6 h, 72 h Method Proliferation assays are performed by treating MOLT4 and CRBN−/− MOLT4 cells with NVP-2 at concentrations indicated for 72 hours. Anti-proliferative effects of NVP-2 are assessed using Cell Titer Glo assay kit. IC50s are determined using Graphpad Prism non-linear regression curve fit. To determine the effect of washout, MOLT4 and CRBN−/− MOLT4 cells are treated with 250 nM NVP-2 or DMSO. After 6 hours, compound is washed from cells by washing cells with media three times where indicated. Cells are allowed to grow for another 66 hours (72-hour total) and assayed for anti-proliferation using Cell Titer Glo assay kit as above.

Cell Assay:^{^[1]}

Cell lines
Wildtype MOLT4 Cells, CRBN−/− MOLT4 Cells
Concentrations
1μM, 500nM, 250nM
Incubation Time
6 h, 72 h
Method
Proliferation assays are performed by treating MOLT4 and CRBN−/− MOLT4 cells with NVP-2 at concentrations indicated for 72 hours. Anti-proliferative effects of NVP-2 are assessed using Cell Titer Glo assay kit. IC50s are determined using Graphpad Prism non-linear regression curve fit. To determine the effect of washout, MOLT4 and CRBN−/− MOLT4 cells are treated with 250 nM NVP-2 or DMSO. After 6 hours, compound is washed from cells by washing cells with media three times where indicated. Cells are allowed to grow for another 66 hours (72-hour total) and assayed for anti-proliferation using Cell Titer Glo assay kit as above.

References

[1] Calla M Olson, et al. Nat Chem Biol. 2018 Feb;14(2):163-170.

Selleck's NVP-2 has been cited by 1 publication

CDK9 inhibition as an effective therapy for small cell lung cancer [ Cell Death Dis, 2024, 15(5):345]	PubMed: 38769311

CDK9 inhibition as an effective therapy for small cell lung cancer [ Cell Death Dis, 2024, 15(5):345]

PubMed: 38769311

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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