Home Cell Cycle CDK inhibitor - Bcl-2 activator - Apoptosis related activator Ro-3306 For research use only.

Ro-3306

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases. RO-3306 enhances p53-mediated Bax activation and mitochondrial apoptosis.

Ro-3306 Chemical Structure

Ro-3306 Chemical Structure

CAS No. 872573-93-8

Purity & Quality Control

Ro-3306 Related Products

Signaling Pathway

CDK Signaling Pathways

CDK Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB-3-1 adenocarcinoma cell line qHTS assay P-glycoprotein substrates identified in KB-3-1 adenocarcinoma cell line, qHTS therapeutic library screen 31515284
human H460a cells Proliferation assay Antiproliferative activity against human H460a cells, IC50=1.04 μM 17303421
human MDA435 cells Proliferation assay Antiproliferative activity against human MDA435 cells, IC50=1.14 μM 17303421
human HCT116 cells Proliferation assay Antiproliferative activity against human HCT116 cells, IC50=1.14 μM 17303421
human RKO cells Proliferation assay Antiproliferative activity against human RKO cells, IC50=2 μM 17303421
human SW480 cells Proliferation assay Antiproliferative activity against human SW480 cells, IC50=2.2 μM 17303421
human SJSA1 cells Proliferation assay Antiproliferative activity against human SJSA1 cells, IC50=4.55 μM 17303421
human HCT116 cells Cytotoxic assay 5 days Cytotoxicity against human HCT116 cells after 5 days by MTS assay， IC50=3.2 μM 22326168
NCI-SNU-1 Growth inhibition assay Inhibition of human NCI-SNU-1 cell growth in a cell viability assay, IC50 = 0.73095 μM. SANGER
H4 Growth inhibition assay Inhibition of human H4 cell growth in a cell viability assay, IC50 = 1.15875 μM. SANGER
PA-1 Growth inhibition assay Inhibition of human PA-1 cell growth in a cell viability assay, IC50 = 1.22654 μM. SANGER
T98G Growth inhibition assay Inhibition of human T98G cell growth in a cell viability assay, IC50 = 1.36812 μM. SANGER
SW1710 Growth inhibition assay Inhibition of human SW1710 cell growth in a cell viability assay, IC50 = 1.61408 μM. SANGER
U251 Growth inhibition assay Inhibition of human U251 cell growth in a cell viability assay, IC50 = 1.63771 μM. SANGER
ALL-PO Growth inhibition assay Inhibition of human ALL-PO cell growth in a cell viability assay, IC50 = 1.90813 μM. SANGER
SK-N-DZ Growth inhibition assay Inhibition of human SK-N-DZ cell growth in a cell viability assay, IC50 = 2.01876 μM. SANGER
A4-Fuk Growth inhibition assay Inhibition of human A4-Fuk cell growth in a cell viability assay, IC50 = 2.05604 μM. SANGER
639-V Growth inhibition assay Inhibition of human 639-V cell growth in a cell viability assay, IC50 = 2.27537 μM. SANGER
Hs-578-T Growth inhibition assay Inhibition of human Hs-578-T cell growth in a cell viability assay, IC50 = 2.27799 μM. SANGER
UM-UC-3 Growth inhibition assay Inhibition of human UM-UC-3 cell growth in a cell viability assay, IC50 = 2.73936 μM. SANGER
BPH-1 Growth inhibition assay Inhibition of human BPH-1 cell growth in a cell viability assay, IC50 = 2.97455 μM. SANGER
5637 Growth inhibition assay Inhibition of human 5637 cell growth in a cell viability assay, IC50 = 3.08532 μM. SANGER
8-MG-BA Growth inhibition assay Inhibition of human 8-MG-BA cell growth in a cell viability assay, IC50 = 3.14049 μM. SANGER
LB1047-RCC Growth inhibition assay Inhibition of human LB1047-RCC cell growth in a cell viability assay, IC50 = 3.23501 μM. SANGER
KOSC-2 Growth inhibition assay Inhibition of human KOSC-2 cell growth in a cell viability assay, IC50 = 3.44662 μM. SANGER
COR-L88 Growth inhibition assay Inhibition of human COR-L88 cell growth in a cell viability assay, IC50 = 3.47583 μM. SANGER
PSN1 Growth inhibition assay Inhibition of human PSN1 cell growth in a cell viability assay, IC50 = 4.10805 μM. SANGER
LXF-289 Growth inhibition assay Inhibition of human LXF-289 cell growth in a cell viability assay, IC50 = 4.2176 μM. SANGER
MN-60 Growth inhibition assay Inhibition of human MN-60 cell growth in a cell viability assay, IC50 = 4.37623 μM. SANGER
NEC8 Growth inhibition assay Inhibition of human NEC8 cell growth in a cell viability assay, IC50 = 4.47076 μM. SANGER
NCI-H2405 Growth inhibition assay Inhibition of human NCI-H2405 cell growth in a cell viability assay, IC50 = 4.74493 μM. SANGER
YH-13 Growth inhibition assay Inhibition of human YH-13 cell growth in a cell viability assay, IC50 = 4.75776 μM. SANGER
SK-MEL-2 Growth inhibition assay Inhibition of human SK-MEL-2 cell growth in a cell viability assay, IC50 = 5.09632 μM. SANGER
NB13 Growth inhibition assay Inhibition of human NB13 cell growth in a cell viability assay, IC50 = 5.32786 μM. SANGER
GAMG Growth inhibition assay Inhibition of human GAMG cell growth in a cell viability assay, IC50 = 5.3715 μM. SANGER
NCI-H1792 Growth inhibition assay Inhibition of human NCI-H1792 cell growth in a cell viability assay, IC50 = 5.47954 μM. SANGER
KP-4 Growth inhibition assay Inhibition of human KP-4 cell growth in a cell viability assay, IC50 = 5.69823 μM. SANGER
HGC-27 Growth inhibition assay Inhibition of human HGC-27 cell growth in a cell viability assay, IC50 = 5.73077 μM. SANGER
GCT Growth inhibition assay Inhibition of human GCT cell growth in a cell viability assay, IC50 = 5.85121 μM. SANGER
PFSK-1 Growth inhibition assay Inhibition of human PFSK-1 cell growth in a cell viability assay, IC50 = 5.86168 μM. SANGER
A172 Growth inhibition assay Inhibition of human A172 cell growth in a cell viability assay, IC50 = 5.9079 μM. SANGER
HO-1-N-1 Growth inhibition assay Inhibition of human HO-1-N-1 cell growth in a cell viability assay, IC50 = 5.95425 μM. SANGER
HN Growth inhibition assay Inhibition of human HN cell growth in a cell viability assay, IC50 = 5.97097 μM. SANGER
IST-MES1 Growth inhibition assay Inhibition of human IST-MES1 cell growth in a cell viability assay, IC50 = 5.97521 μM. SANGER
TGW Growth inhibition assay Inhibition of human TGW cell growth in a cell viability assay, IC50 = 6.05682 μM. SANGER
COR-L23 Growth inhibition assay Inhibition of human COR-L23 cell growth in a cell viability assay, IC50 = 6.3735 μM. SANGER
D-566MG Growth inhibition assay Inhibition of human D-566MG cell growth in a cell viability assay, IC50 = 6.78873 μM. SANGER
SKG-IIIa Growth inhibition assay Inhibition of human SKG-IIIa cell growth in a cell viability assay, IC50 = 6.85572 μM. SANGER
NCI-H446 Growth inhibition assay Inhibition of human NCI-H446 cell growth in a cell viability assay, IC50 = 7.0181 μM. SANGER
KYSE-270 Growth inhibition assay Inhibition of human KYSE-270 cell growth in a cell viability assay, IC50 = 7.03872 μM. SANGER
CAL-39 Growth inhibition assay Inhibition of human CAL-39 cell growth in a cell viability assay, IC50 = 7.3524 μM. SANGER
IST-SL1 Growth inhibition assay Inhibition of human IST-SL1 cell growth in a cell viability assay, IC50 = 7.43349 μM. SANGER
SF539 Growth inhibition assay Inhibition of human SF539 cell growth in a cell viability assay, IC50 = 7.55197 μM. SANGER
TE-1 Growth inhibition assay Inhibition of human TE-1 cell growth in a cell viability assay, IC50 = 7.89091 μM. SANGER
NB10 Growth inhibition assay Inhibition of human NB10 cell growth in a cell viability assay, IC50 = 7.98758 μM. SANGER
8505C Growth inhibition assay Inhibition of human 8505C cell growth in a cell viability assay, IC50 = 7.99817 μM. SANGER
DB Growth inhibition assay Inhibition of human DB cell growth in a cell viability assay, IC50 = 8.01131 μM. SANGER
NCI-H28 Growth inhibition assay Inhibition of human NCI-H28 cell growth in a cell viability assay, IC50 = 8.59884 μM. SANGER
LOXIMVI Growth inhibition assay Inhibition of human LOXIMVI cell growth in a cell viability assay, IC50 = 8.63701 μM. SANGER
ME-180 Growth inhibition assay Inhibition of human ME-180 cell growth in a cell viability assay, IC50 = 8.80395 μM. SANGER
ES3 Growth inhibition assay Inhibition of human ES3 cell growth in a cell viability assay, IC50 = 9.12794 μM. SANGER
SK-N-FI Growth inhibition assay Inhibition of human SK-N-FI cell growth in a cell viability assay, IC50 = 9.41699 μM. SANGER
NBsusSR Growth inhibition assay Inhibition of human NBsusSR cell growth in a cell viability assay, IC50 = 10.2318 μM. SANGER
GI-ME-N Growth inhibition assay Inhibition of human GI-ME-N cell growth in a cell viability assay, IC50 = 10.2783 μM. SANGER
A704 Growth inhibition assay Inhibition of human A704 cell growth in a cell viability assay, IC50 = 10.3318 μM. SANGER
HuP-T4 Growth inhibition assay Inhibition of human HuP-T4 cell growth in a cell viability assay, IC50 = 10.4271 μM. SANGER
A101D Growth inhibition assay Inhibition of human A101D cell growth in a cell viability assay, IC50 = 11.1574 μM. SANGER
MCF7 Growth inhibition assay Inhibition of human MCF7 cell growth in a cell viability assay, IC50 = 11.1849 μM. SANGER
A549 Growth inhibition assay Inhibition of human A549 cell growth in a cell viability assay, IC50 = 11.206 μM. SANGER
HD-MY-Z Growth inhibition assay Inhibition of human HD-MY-Z cell growth in a cell viability assay, IC50 = 11.206 μM. SANGER
HCC1395 Growth inhibition assay Inhibition of human HCC1395 cell growth in a cell viability assay, IC50 = 11.5596 μM. SANGER
SK-NEP-1 Growth inhibition assay Inhibition of human SK-NEP-1 cell growth in a cell viability assay, IC50 = 11.7332 μM. SANGER
HSC-2 Growth inhibition assay Inhibition of human HSC-2 cell growth in a cell viability assay, IC50 = 11.7842 μM. SANGER
HCC1937 Growth inhibition assay Inhibition of human HCC1937 cell growth in a cell viability assay, IC50 = 11.8473 μM. SANGER
RS4-11 Growth inhibition assay Inhibition of human RS4-11 cell growth in a cell viability assay, IC50 = 11.8836 μM. SANGER
CAL-27 Growth inhibition assay Inhibition of human CAL-27 cell growth in a cell viability assay, IC50 = 12.1637 μM. SANGER
NCI-H23 Growth inhibition assay Inhibition of human NCI-H23 cell growth in a cell viability assay, IC50 = 12.4155 μM. SANGER
A431 Growth inhibition assay Inhibition of human A431 cell growth in a cell viability assay, IC50 = 12.7233 μM. SANGER
HT-1080 Growth inhibition assay Inhibition of human HT-1080 cell growth in a cell viability assay, IC50 = 12.7373 μM. SANGER
GB-1 Growth inhibition assay Inhibition of human GB-1 cell growth in a cell viability assay, IC50 = 12.7716 μM. SANGER
BFTC-909 Growth inhibition assay Inhibition of human BFTC-909 cell growth in a cell viability assay, IC50 = 13.1068 μM. SANGER
CAL-33 Growth inhibition assay Inhibition of human CAL-33 cell growth in a cell viability assay, IC50 = 13.6518 μM. SANGER
JVM-3 Growth inhibition assay Inhibition of human JVM-3 cell growth in a cell viability assay, IC50 = 14.2627 μM. SANGER
AN3-CA Growth inhibition assay Inhibition of human AN3-CA cell growth in a cell viability assay, IC50 = 14.9578 μM. SANGER
VM-CUB-1 Growth inhibition assay Inhibition of human VM-CUB-1 cell growth in a cell viability assay, IC50 = 15.1364 μM. SANGER
A253 Growth inhibition assay Inhibition of human A253 cell growth in a cell viability assay, IC50 = 15.2834 μM. SANGER
RPMI-7951 Growth inhibition assay Inhibition of human RPMI-7951 cell growth in a cell viability assay, IC50 = 16.1981 μM. SANGER
NCI-H1563 Growth inhibition assay Inhibition of human NCI-H1563 cell growth in a cell viability assay, IC50 = 16.8253 μM. SANGER
LB831-BLC Growth inhibition assay Inhibition of human LB831-BLC cell growth in a cell viability assay, IC50 = 17.1929 μM. SANGER
ES8 Growth inhibition assay Inhibition of human ES8 cell growth in a cell viability assay, IC50 = 17.3157 μM. SANGER
NCI-H292 Growth inhibition assay Inhibition of human NCI-H292 cell growth in a cell viability assay, IC50 = 17.4749 μM. SANGER
OAW-42 Growth inhibition assay Inhibition of human OAW-42 cell growth in a cell viability assay, IC50 = 17.8823 μM. SANGER
RCC10RGB Growth inhibition assay Inhibition of human RCC10RGB cell growth in a cell viability assay, IC50 = 17.9578 μM. SANGER
PC-3 Growth inhibition assay Inhibition of human PC-3 cell growth in a cell viability assay, IC50 = 18.0043 μM. SANGER
HCC38 Growth inhibition assay Inhibition of human HCC38 cell growth in a cell viability assay, IC50 = 18.1031 μM. SANGER
DEL Growth inhibition assay Inhibition of human DEL cell growth in a cell viability assay, IC50 = 18.387 μM. SANGER
ABC-1 Growth inhibition assay Inhibition of human ABC-1 cell growth in a cell viability assay, IC50 = 18.5627 μM. SANGER
SW756 Growth inhibition assay Inhibition of human SW756 cell growth in a cell viability assay, IC50 = 19.1351 μM. SANGER
TE-6 Growth inhibition assay Inhibition of human TE-6 cell growth in a cell viability assay, IC50 = 19.2103 μM. SANGER
H-EMC-SS Growth inhibition assay Inhibition of human H-EMC-SS cell growth in a cell viability assay, IC50 = 19.4359 μM. SANGER
HCT-116 Growth inhibition assay Inhibition of human HCT-116 cell growth in a cell viability assay, IC50 = 19.668 μM. SANGER
CHP-212 Growth inhibition assay Inhibition of human CHP-212 cell growth in a cell viability assay, IC50 = 19.7478 μM. SANGER
SK-LU-1 Growth inhibition assay Inhibition of human SK-LU-1 cell growth in a cell viability assay, IC50 = 19.753 μM. SANGER
HT-3 Growth inhibition assay Inhibition of human HT-3 cell growth in a cell viability assay, IC50 = 20.028 μM. SANGER
KG-1 Growth inhibition assay Inhibition of human KG-1 cell growth in a cell viability assay, IC50 = 20.206 μM. SANGER
ES1 Growth inhibition assay Inhibition of human ES1 cell growth in a cell viability assay, IC50 = 21.0048 μM. SANGER
Ramos-2G6-4C10 Growth inhibition assay Inhibition of human Ramos-2G6-4C10 cell growth in a cell viability assay, IC50 = 21.7756 μM. SANGER
IA-LM Growth inhibition assay Inhibition of human IA-LM cell growth in a cell viability assay, IC50 = 21.9694 μM. SANGER
BHT-101 Growth inhibition assay Inhibition of human BHT-101 cell growth in a cell viability assay, IC50 = 22.0629 μM. SANGER
OVCAR-4 Growth inhibition assay Inhibition of human OVCAR-4 cell growth in a cell viability assay, IC50 = 22.47 μM. SANGER
SW1573 Growth inhibition assay Inhibition of human SW1573 cell growth in a cell viability assay, IC50 = 22.7934 μM. SANGER
CHL-1 Growth inhibition assay Inhibition of human CHL-1 cell growth in a cell viability assay, IC50 = 23.2833 μM. SANGER
BFTC-905 Growth inhibition assay Inhibition of human BFTC-905 cell growth in a cell viability assay, IC50 = 23.2892 μM. SANGER
TE-12 Growth inhibition assay Inhibition of human TE-12 cell growth in a cell viability assay, IC50 = 23.5782 μM. SANGER
NCI-H358 Growth inhibition assay Inhibition of human NCI-H358 cell growth in a cell viability assay, IC50 = 23.6477 μM. SANGER
OVCAR-5 Growth inhibition assay Inhibition of human OVCAR-5 cell growth in a cell viability assay, IC50 = 24.104 μM. SANGER
CGTH-W-1 Growth inhibition assay Inhibition of human CGTH-W-1 cell growth in a cell viability assay, IC50 = 25.0804 μM. SANGER
SNU-423 Growth inhibition assay Inhibition of human SNU-423 cell growth in a cell viability assay, IC50 = 25.366 μM. SANGER
CP50-MEL-B Growth inhibition assay Inhibition of human CP50-MEL-B cell growth in a cell viability assay, IC50 = 25.7399 μM. SANGER
NCI-H2087 Growth inhibition assay Inhibition of human NCI-H2087 cell growth in a cell viability assay, IC50 = 25.8655 μM. SANGER
RMG-I Growth inhibition assay Inhibition of human RMG-I cell growth in a cell viability assay, IC50 = 25.9219 μM. SANGER
KP-N-YS Growth inhibition assay Inhibition of human KP-N-YS cell growth in a cell viability assay, IC50 = 26.202 μM. SANGER
OMC-1 Growth inhibition assay Inhibition of human OMC-1 cell growth in a cell viability assay, IC50 = 26.508 μM. SANGER
DMS-114 Growth inhibition assay Inhibition of human DMS-114 cell growth in a cell viability assay, IC50 = 27.3966 μM. SANGER
SHP-77 Growth inhibition assay Inhibition of human SHP-77 cell growth in a cell viability assay, IC50 = 27.488 μM. SANGER
MPP-89 Growth inhibition assay Inhibition of human MPP-89 cell growth in a cell viability assay, IC50 = 27.9438 μM. SANGER
NCI-H1581 Growth inhibition assay Inhibition of human NCI-H1581 cell growth in a cell viability assay, IC50 = 28.4535 μM. SANGER
D-283MED Growth inhibition assay Inhibition of human D-283MED cell growth in a cell viability assay, IC50 = 28.4674 μM. SANGER
MOLT-4 Growth inhibition assay Inhibition of human MOLT-4 cell growth in a cell viability assay, IC50 = 28.9072 μM. SANGER
ES6 Growth inhibition assay Inhibition of human ES6 cell growth in a cell viability assay, IC50 = 29.6436 μM. SANGER
NB6 Growth inhibition assay Inhibition of human NB6 cell growth in a cell viability assay, IC50 = 29.8638 μM. SANGER
SW954 Growth inhibition assay Inhibition of human SW954 cell growth in a cell viability assay, IC50 = 29.9407 μM. SANGER
A388 Growth inhibition assay Inhibition of human A388 cell growth in a cell viability assay, IC50 = 29.9422 μM. SANGER
OAW-28 Growth inhibition assay Inhibition of human OAW-28 cell growth in a cell viability assay, IC50 = 30.2278 μM. SANGER
SK-MES-1 Growth inhibition assay Inhibition of human SK-MES-1 cell growth in a cell viability assay, IC50 = 31.2208 μM. SANGER
NCI-H441 Growth inhibition assay Inhibition of human NCI-H441 cell growth in a cell viability assay, IC50 = 31.9868 μM. SANGER
HOP-92 Growth inhibition assay Inhibition of human HOP-92 cell growth in a cell viability assay, IC50 = 32.2356 μM. SANGER
NB14 Growth inhibition assay Inhibition of human NB14 cell growth in a cell viability assay, IC50 = 32.7251 μM. SANGER
MS-1 Growth inhibition assay Inhibition of human MS-1 cell growth in a cell viability assay, IC50 = 32.7604 μM. SANGER
YKG-1 Growth inhibition assay Inhibition of human YKG-1 cell growth in a cell viability assay, IC50 = 32.8336 μM. SANGER
SiHa Growth inhibition assay Inhibition of human SiHa cell growth in a cell viability assay, IC50 = 33.3829 μM. SANGER
HAL-01 Growth inhibition assay Inhibition of human HAL-01 cell growth in a cell viability assay, IC50 = 34.2376 μM. SANGER
Ca-Ski Growth inhibition assay Inhibition of human Ca-Ski cell growth in a cell viability assay, IC50 = 34.7669 μM. SANGER
NCI-H747 Growth inhibition assay Inhibition of human NCI-H747 cell growth in a cell viability assay, IC50 = 34.9875 μM. SANGER
KYSE-510 Growth inhibition assay Inhibition of human KYSE-510 cell growth in a cell viability assay, IC50 = 35.6485 μM. SANGER
A204 Growth inhibition assay Inhibition of human A204 cell growth in a cell viability assay, IC50 = 35.7209 μM. SANGER
HSC-3 Growth inhibition assay Inhibition of human HSC-3 cell growth in a cell viability assay, IC50 = 36.0086 μM. SANGER
HuP-T3 Growth inhibition assay Inhibition of human HuP-T3 cell growth in a cell viability assay, IC50 = 36.4832 μM. SANGER
RPMI-8226 Growth inhibition assay Inhibition of human RPMI-8226 cell growth in a cell viability assay, IC50 = 36.6208 μM. SANGER
QIMR-WIL Growth inhibition assay Inhibition of human QIMR-WIL cell growth in a cell viability assay, IC50 = 36.644 μM. SANGER
HuH-7 Growth inhibition assay Inhibition of human HuH-7 cell growth in a cell viability assay, IC50 = 38.6184 μM. SANGER
CAL-12T Growth inhibition assay Inhibition of human CAL-12T cell growth in a cell viability assay, IC50 = 38.9416 μM. SANGER
GT3TKB Growth inhibition assay Inhibition of human GT3TKB cell growth in a cell viability assay, IC50 = 39.1921 μM. SANGER
OC-314 Growth inhibition assay Inhibition of human OC-314 cell growth in a cell viability assay, IC50 = 39.7125 μM. SANGER
NCI-H2228 Growth inhibition assay Inhibition of human NCI-H2228 cell growth in a cell viability assay, IC50 = 40.1403 μM. SANGER
NCI-H727 Growth inhibition assay Inhibition of human NCI-H727 cell growth in a cell viability assay, IC50 = 40.2531 μM. SANGER
NTERA-S-cl-D1 Growth inhibition assay Inhibition of human NTERA-S-cl-D1 cell growth in a cell viability assay, IC50 = 40.4706 μM. SANGER
MMAC-SF Growth inhibition assay Inhibition of human MMAC-SF cell growth in a cell viability assay, IC50 = 40.9235 μM. SANGER
HTC-C3 Growth inhibition assay Inhibition of human HTC-C3 cell growth in a cell viability assay, IC50 = 41.4169 μM. SANGER
SF295 Growth inhibition assay Inhibition of human SF295 cell growth in a cell viability assay, IC50 = 41.5379 μM. SANGER
U-2-OS Growth inhibition assay Inhibition of human U-2-OS cell growth in a cell viability assay, IC50 = 41.8567 μM. SANGER
HPAF-II Growth inhibition assay Inhibition of human HPAF-II cell growth in a cell viability assay, IC50 = 41.9382 μM. SANGER
KYSE-450 Growth inhibition assay Inhibition of human KYSE-450 cell growth in a cell viability assay, IC50 = 42.4707 μM. SANGER
DMS-273 Growth inhibition assay Inhibition of human DMS-273 cell growth in a cell viability assay, IC50 = 43.2836 μM. SANGER
HT-1376 Growth inhibition assay Inhibition of human HT-1376 cell growth in a cell viability assay, IC50 = 43.3156 μM. SANGER
786-0 Growth inhibition assay Inhibition of human 786-0 cell growth in a cell viability assay, IC50 = 43.6261 μM. SANGER
NCI-H810 Growth inhibition assay Inhibition of human NCI-H810 cell growth in a cell viability assay, IC50 = 44.0136 μM. SANGER
8305C Growth inhibition assay Inhibition of human 8305C cell growth in a cell viability assay, IC50 = 44.645 μM. SANGER
DSH1 Growth inhibition assay Inhibition of human DSH1 cell growth in a cell viability assay, IC50 = 45.7042 μM. SANGER
KYSE-520 Growth inhibition assay Inhibition of human KYSE-520 cell growth in a cell viability assay, IC50 = 46.5721 μM. SANGER
TYK-nu Growth inhibition assay Inhibition of human TYK-nu cell growth in a cell viability assay, IC50 = 46.7092 μM. SANGER
EPLC-272H Growth inhibition assay Inhibition of human EPLC-272H cell growth in a cell viability assay, IC50 = 46.7283 μM. SANGER
T-24 Growth inhibition assay Inhibition of human T-24 cell growth in a cell viability assay, IC50 = 47.9292 μM. SANGER
BHY Growth inhibition assay Inhibition of human BHY cell growth in a cell viability assay, IC50 = 47.999 μM. SANGER
NCI-H82 Growth inhibition assay Inhibition of human NCI-H82 cell growth in a cell viability assay, IC50 = 48.217 μM. SANGER
SF268 Growth inhibition assay Inhibition of human SF268 cell growth in a cell viability assay, IC50 = 48.6189 μM. SANGER
C-33-A Growth inhibition assay Inhibition of human C-33-A cell growth in a cell viability assay, IC50 = 48.7554 μM. SANGER
MIA-PaCa-2 Growth inhibition assay Inhibition of human MIA-PaCa-2 cell growth in a cell viability assay, IC50 = 48.8388 μM. SANGER
HCC1954 Growth inhibition assay Inhibition of human HCC1954 cell growth in a cell viability assay, IC50 = 49.0871 μM. SANGER
NCI-H1666 Growth inhibition assay Inhibition of human NCI-H1666 cell growth in a cell viability assay, IC50 = 49.3642 μM. SANGER
GR-ST Growth inhibition assay Inhibition of human GR-ST cell growth in a cell viability assay, IC50 = 49.3695 μM. SANGER
Ca9-22 Growth inhibition assay Inhibition of human Ca9-22 cell growth in a cell viability assay, IC50 = 49.4013 μM. SANGER
D-502MG Growth inhibition assay Inhibition of human D-502MG cell growth in a cell viability assay, IC50 = 49.417 μM. SANGER
SCC-4 Growth inhibition assay Inhibition of human SCC-4 cell growth in a cell viability assay, IC50 = 49.5602 μM. SANGER
RKO Growth inhibition assay Inhibition of human RKO cell growth in a cell viability assay, IC50 = 49.8356 μM. SANGER
Click to View More Cell Line Experimental Data

Biological Activity

Description RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases. RO-3306 enhances p53-mediated Bax activation and mitochondrial apoptosis.
Targets
CDK1 [1]
(Cell-free assay)		 PKCδ [1]
(Cell-free assay)		 SGK [1]
(Cell-free assay)		 ERK [1]
(Cell-free assay)
20 nM(Ki) 318 nM(Ki) 497 nM(Ki) 1980 nM(Ki)
In vitro
In vitro

RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).[1]

RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.[2]
Kinase Assay CDK assay
The activity of CDK1 cyclin B1, CDK1 cyclin A, CDK2 cyclin E, and CDK4 cyclin D is measured by a homogeneous time-resolved fluorescence assay in a 96-well format. The assay buffer contained 25 mM Hepes, 6.25 mM MgCl2, 0.003% Tween 20, 0.3 mg/mL BSA, 1.5 mM DTT, and ATP as follows: 162 μM (CDK1), 90 mM (CDK2), or 135 μM (CDK4). CDK1 and CDK2 buffer contained 10 mM MgCl2. Test compounds are diluted in assay buffer to 3-fold their final concentration in 20 μL, and the reaction is started by the addition of a 40 μL assay buffer containing the pRB substrate (0.185μM). The plates are incubated at 37°C for 30 min with constant agitation, and the reaction is terminated by the addition of 15 μL of 1.6μM anti-phospho pRB antibody (Ser-780) in 25 mM Hepes, 24 mM EDTA, and 0.2 mg/mL BSA. After an additional 30 min of incubation with shaking, 15μL of 3nM Lance-Eu-W1024-labeledanti-rabbitIgG and 60 nM Alophycocyanin-conjugated anti-His-6 antibody in 25 mM Hepes, and 0.5 mg/mL BSA is added and incubated for 1 h. The plates are read in the Victor-V multi- label reader at excitation 340 nm and emission 615 nm and 665 nm. The IC50 values are calculated from the readings at 665 nm and normalized for Europium readings at 615 nm. Ki values are calculated according to the equation: Ki= IC50/(1 + S/Km ), where S is the ATP concentration in the assay and Km is the Michaelis-Menten constant for ATP. The inhibitory activity against the panel of kinases is determined by the IMAP assay technology.
Cell Research Cell lines MDA-MB-231 cell line
Concentrations 20 μM
Incubation Time 72 h
Method

Log phases cells (25,000) are seeed in 96-well plates and incubated in a 37℃ incubator with CO2, After 24 h, different concentrations of RO-3306 are administered to determine the drug concentrations required to achieve a 50% growth inhibition (IC50). MTT (20 μL, 5mg/mL stock solution in saline) is added to each well and the cells are incubated for 4 h. Supernatants are removed and formazan crystals from viable cells are solubilized with 200 μL anhydrous DMSO. The absorbance is detected with a 550 model microplate reader at the 565 nm wavelength.
Experimental Result Images Methods Biomarkers Images PMID
Western blot p-Cdk1 / Cdk1 / Cleaved PARP / Cleaved caspase-3 / p-p38 / Bad / Apaf1-1 / p-H2Ax / Bim / Bad 27385216
Immunofluorescence PARP1 / Plk1 28069876
Growth inhibition assay Cell viability 27385216
In Vivo
In vivo

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor.
Animal Research Animal Models Female BALB/c mice
Dosages 1.5 mg/kg
Administration i.n.

Chemical Information & Solubility

Molecular Weight 351.45 Formula

C18H13N3OS2
CAS No. 872573-93-8 SDF Download Ro-3306 SDF
Smiles C1=CC2=C(C=CC(=C2)C=C3C(=O)NC(=NCC4=CC=CS4)S3)N=C1
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 70 mg/mL ( (199.17 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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