Home DNA Damage/DNA Repair DNA/RNA Synthesis chemical - Ferroptosis activator - Autophagy activator Cisplatin For research use only.

Cisplatin

Synonyms: NSC 119875, Cisplatinum, cis-diamminedichloroplatinum II, CDDP, cis DDP, DDP

Cisplatin is an inorganic platinum complex, which is able to inhibit DNA synthesis by conforming DNA adducts in tumor cells. Cisplatin activates ferroptosis and induces autophagy.Solutions are unstable and should be fresh-prepared.DMSO is not recommended to dissolve platinum-based drugs, which can easily lead to drug inactivation.

Cisplatin Chemical Structure

Cisplatin Chemical Structure

CAS No. 15663-27-1

Purity & Quality Control

Cisplatin Related Products

Signaling Pathway

DNA/RNA Synthesis Signaling Pathways

DNA/RNA Synthesis Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Human osteosarcoma cells (HOS, 143B, U2OS and MG‑63) Cell cycle analysis 2 μM 48 h Cisplatin treatment markedly increased the G2/M population in all cell lines. 31059083
OVC cells (A2780, TOV-112D, and cis-A2780) Cell Cytotoxicity Assay 0.5, 1, 2.5, 5, 10, 20, and 50 μM 48 h Combination of cisplatin and MEK inhibitor cobimetinib (10 nM) enhances cell death in three ovarian cancer cell lines (A2780, TOV-112D, and cis-A2780). 31057611
HCC cell lines HepG2 and Huh7 Cell viability assay 0-30 μM 48 h CD133+ HCC cells exhibit resistance to cisplatin. 31056532
Saos-2 cells qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells 29435139
OHS-50 cells qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells 29435139
SK-N-MC cells qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells 29435139
Click to View More Cell Line Experimental Data

Biological Activity

Description Cisplatin is an inorganic platinum complex, which is able to inhibit DNA synthesis by conforming DNA adducts in tumor cells. Cisplatin activates ferroptosis and induces autophagy.Solutions are unstable and should be fresh-prepared.DMSO is not recommended to dissolve platinum-based drugs, which can easily lead to drug inactivation.
Features One of the most widely used and most potent chemotherapeutic agents. This product is not recommended to be dissolved in dimethylsulfoxide (DMSO).[7]
Targets
DNA synthesis [1]
(Tumor cells)
In vitro
In vitro

Cisplatin induces cytotoxic by interaction with DNA to form DNA adducts which activate several signal transduction pathways, including Erk, p53, p73, and MAPK, which culminates in the activation of apoptosis. [1]

Cisplatin (30 μM) treated for 6 h induces an apparent activation of Erk in HeLa cells, which is sustained over the following 14 h period. Cisplatin also shows an effective antineoplastic activity by inducing tumor cells death[2].

Cisplatin displays ability to cause renal proximal tubular cell (RPTC) apoptosis, causing cell shrinkage, a 50-fold increase in caspase 3 activity, a 4-fold increase in phosphatidylserine externalization, and 5- and 15-fold increases in chromatin condensation and DNA hypoploidy, respectively. [4]

Cisplatin (800 μM) causes typical features of necrosis of RPTC after treatment for 4 hr. [5]
Cell Research Cell lines Leukemia L1210/0 cells
Concentrations 7 μg/mL
Incubation Time 2 hours
Method

L1210/0 cells are maintained in an exponential suspension culture at 37 ℃ in a humidified atmosphere of 5% CO2 in McCoy's medium 5a (modified), supplemented with 15% calfserum, and Fungizone. L1210/0 cells are incubated in Cisplatin (7 μg/mL) for 2 hr at 37 ℃. To measure growth inhibition, the cells are centrifuged, washed once, resuspended in fresh medium at 30 × 103 to 50 × 103 cells/mL, and incubated for 3 days. Cell numbers are determined on a Coulter Counter. An aliquot of cells is diluted with an equal volume of 0.4% trypan blue. Viability is recorded as the percentage of cells that has excluded trypan blue. Cells incubated with Cisplatin as above are also diluted into 0.1% agar and allowed to grow for 2 weeks when colonies are counted.
Experimental Result Images Methods Biomarkers Images PMID
Western blot ATF3 FEN1 PD-L1 / p-MEK / MEK / p-STAT3 / STAT3 LC3B-I / LC3B-II / Beclin-1 p-AMPK / AMPK / p-mTOR / mTOR 20651982
Immunofluorescence H2A.X / RPA γ-H2A.X / 53BP1 N-cadherin / E-cadherin / Vimentin LC3B 28993682
Growth inhibition assay Cell viability 26062553
In Vivo
In vivo

Cisplatin has been demonstrated to be efficient in regression tumor growth in a wide variety of animal tumors models, including head and neck cancer xenografts, cervical squamous carcinoma xenografts, testicular carcinoma xenografts, ovarian cancer xenografts, breast carcinoma xenografts, colonic carcinoma, heterotransplanted hepatoblastoma, and so on. Cisplatin (5 mg/kg) given weekly i.v. at the day 1 and 7 induces a tumor growth inhibition (GI) of 77.5% and 85.1% of the serous xenografts Ov.Ri(C) and OVCAR-3, respectively. [6]
Animal Research Animal Models Female NMRI/Cpb (nuinu) mice
Dosages 5 mg/kg
Administration i.v.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06356155 Not yet recruiting
Urothelial Carcinoma
University of Michigan Rogel Cancer Center
 October 2024 Phase 2
NCT06393816 Not yet recruiting
Large Cell Neuroendocrine Carcinoma of the Lung
Centre Leon Berard|Groupe Français de Pneumo-Cancérologie
 May 2024 Phase 2
NCT06406465 Not yet recruiting
Carcinoma Neuroendocrine|Tumor Neuroendocrine|Tumors Neuroendocrine|Neuroendocrine; Carcinoma|Small Cell; Receptors
National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC)
 May 15 2024 Phase 2
NCT04915183 Recruiting
Hearing Loss|Head and Neck Cancer
National Institute on Deafness and Other Communication Disorders (NIDCD)|National Institutes of Health Clinical Center (CC)
 May 15 2024 Phase 2

Chemical Information & Solubility

Molecular Weight 300.05 Formula

Cl2H6N2Pt
CAS No. 15663-27-1 SDF Download Cisplatin SDF
Smiles [NH2-].[NH2-].Cl[Pt+2]Cl
Storage (From the date of receipt) 2 years 4°C(in the dark) powder Solutions are unstable. Prepare fresh or purchase small, pre-packaged sizes. Repackage upon receipt.

In vitro
Batch:

DMF : 15 mg/mL

Water : 1.5 mg/mL

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Frequently Asked Questions

Question 1:
What is the appropriate concentration of DMF for cell culture and animal study?

Answer:
It depends on the cell type. The final concentration of DMF should be better limited to less than 0.1% if possible, or below 1%. Using saline as a vehicle for cisplatin at up to 3mg/ml is recommended. it's a suspension and can be administrated via oral gavage.

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