Home DNA Damage/DNA Repair DNA/RNA Synthesis inhibitor - CRISPR/Cas9 activator SCR7 For research use only.

SCR7

SCR7 is a specific DNA Ligase IV inhibitor, which blocks nonhomologous end-joining (NHEJ). It increases the efficiency of HDR-mediated genome editing up to 19-fold using CRISPR/Cas9 in mammalian cells and mouse embryos.

SCR7 Chemical Structure

SCR7 Chemical Structure

CAS No. 14892-97-8

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SCR7 Related Products

Signaling Pathway

DNA/RNA Synthesis Signaling Pathways

DNA/RNA Synthesis Signaling Pathway

Biological Activity

Description SCR7 is a specific DNA Ligase IV inhibitor, which blocks nonhomologous end-joining (NHEJ). It increases the efficiency of HDR-mediated genome editing up to 19-fold using CRISPR/Cas9 in mammalian cells and mouse embryos.
Targets
DNA Ligase IV [1]
In vitro
In vitro

SCR7 effectively inhibits the formation of multimers at 200 μM and above. SCR7 successfully inhibits cell proliferation of MCF7, A549, HeLa, T47D, A2780, HT1080, and Nalm6 with IC50 of 40, 34, 44, 8.5, 120, 10, and 50 μM, respectively.[1] SCR7 suppresses the NHEJ repair of CRISPR-Cas9-induced DSBs.[2]SCR7 increases the efficiency of HDR-mediated genome editing up to 19-fold using CRISPR/Cas9 in mammalian cells and mouse embryos[3].
Kinase Assay Complementation of SCR7 Inhibition with Purified Ligase IV
Complementation experiment is carried out by adding increasing concentrations of purified Ligase IV/XRCC4 complex (30, 60, and 120 fmol) along with the oligomeric DNA substrates (5’ compatible and 5’-5’ noncompatible ends) to the SCR7-treatedextracts. Reactions are incubated for 2 h at 25℃. The reaction products are then resolved on 8% denaturing PAGE. The gel is dried and exposed and the signal is detected with a PhosphorImager and analyzed with Multi Gauge (V3.0) software.
Cell Research Cell lines MCF7, CEM, HeLa, A549, HT1080, A2780, T47D, Nalm6, N114 and K562 cells
Concentrations 250 μM
Incubation Time 48 h
Method

Cell proliferation of cancer cells are determined by MTT and trypan blue assays. Briefly, MCF7, CEM, HeLa, A549, HT1080, A2780, T47D, Nalm6, N114 and K562 cells are grown in presence of SCR7 (10, 50, 100, and 250 μM) for 24 or 48 h, and subjected to MTT or trypan blue assays. Each experiment is repeated a minimum of three independent times.
In Vivo
In vivo

SCR7 treatment (10 mg/kg, i.m.) significantly reduces breast adenocarcinoma-induced tumor, and exhibits 4-fold increase in lifespan compared with control group. However, in Swiss albino mice with Dalton’s lymphoma tumor model, SCR7 (20 mg/kg, i.p.) exhibits neither tumor regression nor increase in lifespan. In BALB/c mice, SCR7 (20 mg/kg, i.p.) significantly enhances the cytotoxic effects of radiation, VP-16213 and 3-Aminobenzamide on tumor derived from Dalton’s lymphoma (DLA) cells.[1]
Animal Research Animal Models BALB/c mice
Dosages 10 mg/kg
Administration i.m.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03941483 Completed
Acute Kidney Injury (AKI)
Astellas Pharma Inc
 November 1 2019 Phase 2

Chemical Information & Solubility

Molecular Weight 332.38 Formula

C18H12N4OS
CAS No. 14892-97-8 SDF Download SCR7 SDF
Smiles C1=CC=C(C=C1)C2=NC3=C(NC(=S)NC3=O)N=C2C4=CC=CC=C4
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 66 mg/mL ( (198.56 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 23 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
What should I do if I got precipitate out of solution after a freeze thaw and cannot re-suspend it?

Answer:
Some compounds will precipitate out of solution after freeze/thaw, especially at very high concentration. You can warm it up to 45 degree and sonicate it to help it dissolve, of course, adding more DMSO will help too.

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