Home DNA Damage/DNA Repair DNA/RNA Synthesis inhibitor Madrasin For research use only.

Madrasin

Synonyms: DDD00107587

Madrasin (DDD00107587) is a potent and cell-penetrant pre-mRNA splicing inhibitor. Madrasin interferes with the early stages of spliceosome assembly and stalls spliceosome assembly at the A complex.

Madrasin Chemical Structure

Madrasin Chemical Structure

CAS No. 374913-63-0

Purity & Quality Control

Batch: S028001 Ethanol]3 mg/mL]false]DMSO]1 mg/mL]false]Water]Insoluble]false Purity: 99.97%
99.97

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Signaling Pathway

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DNA/RNA Synthesis Signaling Pathway

Biological Activity

Description Madrasin (DDD00107587) is a potent and cell-penetrant pre-mRNA splicing inhibitor. Madrasin interferes with the early stages of spliceosome assembly and stalls spliceosome assembly at the A complex.
In vitro
In vitro

Madrasin interferes with the early stages of spliceosome assembly and stalls spliceosome assembly at the A complex. Madrasin is cytotoxic at higher concentrations, although at lower concentrations it induces cell cycle arrest, promotes a specific reorganization of subnuclear protein localization, and modulates splicing of multiple pre-mRNAs in both HeLa and HEK293 cells.[1]
Cell Research Cell lines HeLa cells, HEK293 cells
Concentrations 10-30 μM
Incubation Time 2-24 h
Method

HeLa cells are seeded in 6-well plates, and after cells are treated with Madrasin. The cells are harvested after 4, 8, and 24 h and then washed twice with PBS, resuspended in ice-cold 70% ethanol, and fixed at room temperature for 30 min. Fixed cells are used for flow cytometry analysis.

Chemical Information & Solubility

Molecular Weight 311.34 Formula

C16H17N5O2
CAS No. 374913-63-0 SDF --
Smiles CC1=C(N=C(NC1=O)NC2=NC(=C3C=CC(=CC3=N2)OC)C)C
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

Ethanol : 3 mg/mL

DMSO : 1 mg/mL ( (3.21 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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