Home DNA Damage/DNA Repair DNA/RNA Synthesis inhibitor PFM01 For research use only.

PFM01

PFM01, a cell-permeable N-alkylated Mirin derivative, is a selective inhibitor of MRE11 endonuclease activity.

PFM01 Chemical Structure

PFM01 Chemical Structure

CAS No. 1558598-41-6

Purity & Quality Control

Batch: S354901 DMSO]59 mg/mL]false]Ethanol]59 mg/mL]false]Water]Insoluble]false Purity: 99.75%
99.75

PFM01 Related Products

Signaling Pathway

DNA/RNA Synthesis Signaling Pathways

DNA/RNA Synthesis Signaling Pathway

Biological Activity

Description PFM01, a cell-permeable N-alkylated Mirin derivative, is a selective inhibitor of MRE11 endonuclease activity.
Targets
MRE11 [1]
In vitro
In vitro

PFM01 rescues the repair defect in 48BR (WT) and HSC62 (BRCA2-defective) primary cells, diminishes the RAD51 foci formation in 1BR3 (WT) and HSC62 (BRCA2-defective) cells, enhances non-homologous end-joining (NHEJ) in H1299 dA3 cells and reduces homologous recombination (HR) in U2OS DR-GFP cells, also substantially relieves the double-strand break (DSB) repair defect confers by mirin or PFM39 in irradiated G2 cells.[1]
Cell Research Cell lines 48BR (human primary fibroblasts), ATLD2 (primary fibroblasts), 1BR3 hTERT, HSC62 hTERT (BRCA2-defective) and 2BN hTERT (XLF-defective)
Concentrations 30 min
Incubation Time 100 μM
Method

48BR (human primary fibroblasts), ATLD2 (primary fibroblasts), 1BR3 hTERT, HSC62 hTERT (BRCA2-defective) and 2BN hTERT (XLF-defective), were cultured in Dulbecco’s modification of Eagles medium with 15% fetal calf serum (FCS). A549 cells were cultured in minimal essential medium (MEM) with 10% FCS. Cells were irradiated in medium using a 137Cs source. MRE11 inhibitor concentrations were 500 μM Mirin, 100 μM PFM39, 100 μM PFM01 and 100 μM PFM03, unless otherwise stated. 10 μM ATM inhibitor (KU55933) or 10 μM DNA-PKcs inhibitor (NU7441) were added 30 min prior to irradiation unless indicated.

Chemical Information & Solubility

Molecular Weight 293.40 Formula

C14H15NO2S2
CAS No. 1558598-41-6 SDF --
Smiles CC(C)CN1C(=S)S\C(=C/C2=CC=C(O)C=C2)C1=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 59 mg/mL ( (201.09 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 59 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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