PFM01

Catalog No.S3549 Batch:S354901

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Technical Data

Formula

C14H15NO2S2
Molecular Weight 293.40 CAS No. 1558598-41-6
Solubility (25°C)* In vitro DMSO 59 mg/mL (201.09 mM)
Ethanol 59 mg/mL (201.09 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description PFM01, a cell-permeable N-alkylated Mirin derivative, is a selective inhibitor of MRE11 endonuclease activity.
Targets
MRE11 [1]
In vitro

PFM01 rescues the repair defect in 48BR (WT) and HSC62 (BRCA2-defective) primary cells, diminishes the RAD51 foci formation in 1BR3 (WT) and HSC62 (BRCA2-defective) cells, enhances non-homologous end-joining (NHEJ) in H1299 dA3 cells and reduces homologous recombination (HR) in U2OS DR-GFP cells, also substantially relieves the double-strand break (DSB) repair defect confers by mirin or PFM39 in irradiated G2 cells.[1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    48BR (human primary fibroblasts), ATLD2 (primary fibroblasts), 1BR3 hTERT, HSC62 hTERT (BRCA2-defective) and 2BN hTERT (XLF-defective)

  • Concentrations

    30 min

  • Incubation Time

    100 μM

  • Method

    48BR (human primary fibroblasts), ATLD2 (primary fibroblasts), 1BR3 hTERT, HSC62 hTERT (BRCA2-defective) and 2BN hTERT (XLF-defective), were cultured in Dulbecco’s modification of Eagles medium with 15% fetal calf serum (FCS). A549 cells were cultured in minimal essential medium (MEM) with 10% FCS. Cells were irradiated in medium using a 137Cs source. MRE11 inhibitor concentrations were 500 μM Mirin, 100 μM PFM39, 100 μM PFM01 and 100 μM PFM03, unless otherwise stated. 10 μM ATM inhibitor (KU55933) or 10 μM DNA-PKcs inhibitor (NU7441) were added 30 min prior to irradiation unless indicated.

Selleck's PFM01 has been cited by 1 publication

ATR protects ongoing and newly assembled DNA replication forks through distinct mechanisms [ Cell Rep, 2023, 42(7):112792] PubMed: 37454295

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.