Cisplatin

Catalog No.S1166 Batch:S116614

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Technical Data

Formula

Cl2H6N2Pt
Molecular Weight 300.05 CAS No. 15663-27-1
Solubility (25°C)* In vitro Water 3 mg/mL (9.99 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Cisplatin is an inorganic platinum complex, which is able to inhibit DNA synthesis by conforming DNA adducts in tumor cells. Cisplatin activates ferroptosis and induces autophagy.Solutions are unstable and should be fresh-prepared.DMSO is not recommended to dissolve platinum-based drugs, which can easily lead to drug inactivation.
Targets
DNA synthesis [1]
(Tumor cells)
In vitro

Cisplatin induces cytotoxic by interaction with DNA to form DNA adducts which activate several signal transduction pathways, including Erk, p53, p73, and MAPK, which culminates in the activation of apoptosis. [1]

Cisplatin (30 μM) treated for 6 h induces an apparent activation of Erk in HeLa cells, which is sustained over the following 14 h period. Cisplatin also shows an effective antineoplastic activity by inducing tumor cells death[2].

Cisplatin displays ability to cause renal proximal tubular cell (RPTC) apoptosis, causing cell shrinkage, a 50-fold increase in caspase 3 activity, a 4-fold increase in phosphatidylserine externalization, and 5- and 15-fold increases in chromatin condensation and DNA hypoploidy, respectively. [4]

Cisplatin (800 μM) causes typical features of necrosis of RPTC after treatment for 4 hr. [5]
In vivo

Cisplatin has been demonstrated to be efficient in regression tumor growth in a wide variety of animal tumors models, including head and neck cancer xenografts, cervical squamous carcinoma xenografts, testicular carcinoma xenografts, ovarian cancer xenografts, breast carcinoma xenografts, colonic carcinoma, heterotransplanted hepatoblastoma, and so on. Cisplatin (5 mg/kg) given weekly i.v. at the day 1 and 7 induces a tumor growth inhibition (GI) of 77.5% and 85.1% of the serous xenografts Ov.Ri(C) and OVCAR-3, respectively. [6]
Features One of the most widely used and most potent chemotherapeutic agents. This product is not recommended to be dissolved in dimethylsulfoxide (DMSO).[7]

Protocol (from reference)

Cell Assay:

[3]
  • Cell lines

    Leukemia L1210/0 cells

  • Concentrations

    7 μg/mL

  • Incubation Time

    2 hours

  • Method

    L1210/0 cells are maintained in an exponential suspension culture at 37 ℃ in a humidified atmosphere of 5% CO2 in McCoy's medium 5a (modified), supplemented with 15% calfserum, and Fungizone. L1210/0 cells are incubated in Cisplatin (7 μg/mL) for 2 hr at 37 ℃. To measure growth inhibition, the cells are centrifuged, washed once, resuspended in fresh medium at 30 × 103 to 50 × 103 cells/mL, and incubated for 3 days. Cell numbers are determined on a Coulter Counter. An aliquot of cells is diluted with an equal volume of 0.4% trypan blue. Viability is recorded as the percentage of cells that has excluded trypan blue. Cells incubated with Cisplatin as above are also diluted into 0.1% agar and allowed to grow for 2 weeks when colonies are counted.
Animal Study:

[7]
  • Animal Models

    Female NMRI/Cpb (nuinu) mice

  • Dosages

    5 mg/kg

  • Administration

    i.v.

Customer Product Validation

Data from [Cancer Res, 2014, 74(1), 298-308]

Data from [PLoS One, 2013, 8(1), e54595]

, 2013, Dr. Edita Aksamitiene from Thomas Jefferson University

Data from [Data independently produced by , , Curr Cancer Drug Targets, 2016, 16(7):631-8]

Selleck's Cisplatin has been cited by 805 publications

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Early evolutionary branching across spatial domains predisposes to clonal replacement under chemotherapy in neuroblastoma [ Nat Commun, 2024, 15(1):8992] PubMed: 39419962
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Comprehensive multi-omics analysis reveals WEE1 as a synergistic lethal target with hyperthermia through CDK1 super-activation [ Nat Commun, 2024, 15(1):2089] PubMed: 38453961
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Modeling lung adenocarcinoma metastases using patient-derived organoids [ Cell Rep Med, 2024, 5(10):101777] PubMed: 39413736
Personalized drug screening using patient-derived organoid and its clinical relevance in gastric cancer [ Cell Rep Med, 2024, 5(7):101627] PubMed: 38964315

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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