Home Metabolism Dehydrogenase inhibitor 6AN (6-Aminonicotinamide) For research use only.

6AN (6-Aminonicotinamide)

6AN (6-Aminonicotinamide) is an antimetabolite used to inhibit the NADPH-producing pentose phosphate pathway (PPP) in many cellular systems, making them more susceptible to oxidative stress. 6-Aminonicotinamide is a competitive inhibitor of NADP+-dependent enzyme glucose-6-phosphate dehydrogenase (G6PD) with Ki of 0.46 μM.

6AN (6-Aminonicotinamide) Chemical Structure

6AN (6-Aminonicotinamide) Chemical Structure

CAS No. 329-89-5

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6AN (6-Aminonicotinamide) Related Products

Signaling Pathway

Dehydrogenase Signaling Pathways

Dehydrogenase Signaling Pathway

Biological Activity

Description 6AN (6-Aminonicotinamide) is an antimetabolite used to inhibit the NADPH-producing pentose phosphate pathway (PPP) in many cellular systems, making them more susceptible to oxidative stress. 6-Aminonicotinamide is a competitive inhibitor of NADP+-dependent enzyme glucose-6-phosphate dehydrogenase (G6PD) with Ki of 0.46 μM.
Targets
G6PD [1]
(Cell-free assay)
0.46 μM(Ki)
In vitro
In vitro

Blocking the pentose phosphate pathway (PPP) by 6-AN significantly inhibits mES colony formation in the presence of a normal concentration of glucose. 6-AN treatment effectively inhibits AKT phosphorylation and activation of its downstream effector S6. 6-AN treatment has no effect on the ERK and PDK1 activities, suggesting an AKT-specific effect. 6-AN treatment inhibits cell growth.[2]
Cell Research Cell lines HeLa cells, mES cells
Concentrations 100 nM
Incubation Time 24 h, 2 days, 3 days, 4 days
Method

PHLDA3 knockout blocks the effects of 6-AN, R5P or uridine on cell growth in vitro. Isogenic PHLDA3 WT and knockout HeLa cells are seeded in 96-well plates at a density of 1000 cells/well. After 48 h, the cells are treated with 6-AN (100 nM). Cell viability is detected by CCK-8 assays at indicated time points.
In Vivo
In vivo

PTEN null human cancer cells and in vivo murine models are sensitive to 6-AN (anti-PPP, anti pentose phosphate pathway) treatments, suggesting the importance of the PPP in maintaining AKT activation even in the presence of a constitutively activated PI3K pathway. The treatment of TALL models with 6-AN significantly increases the Phlda3 mRNA and protein levels, accompanied by substantial decreases in the levels of P-AKT and P-S6 as well as the PPP metabolic intermediates.[2]
Animal Research Animal Models male CAnN.Cg-Foxn1nu/CrlVr mice
Dosages 1 mg/kg, 15 mg/kg
Administration IP

Chemical Information & Solubility

Molecular Weight 137.14 Formula

C6H7N3O
CAS No. 329-89-5 SDF --
Smiles NC(=O)C1=CC=C(N)N=C1
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 27 mg/mL ( (196.87 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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