Home Metabolism Dehydrogenase inhibitor - NADPH-oxidase inhibitor G6PDi-1 For research use only.

G6PDi-1

G6PDi-1 is an effective inhibitor of glucose-6-phosphate dehydrogenase (G6PD). G6PDi-1 depletes NADPH and decreases inflammatory cytokine production.

G6PDi-1 Chemical Structure

G6PDi-1 Chemical Structure

CAS No. 2457232-14-1

Purity & Quality Control

G6PDi-1 Related Products

Signaling Pathway

Dehydrogenase Signaling Pathways

Dehydrogenase Signaling Pathway

Biological Activity

Description G6PDi-1 is an effective inhibitor of glucose-6-phosphate dehydrogenase (G6PD). G6PDi-1 depletes NADPH and decreases inflammatory cytokine production.
Targets
G6PD [1] NADPH [1]
In vitro
In vitro

G6PDi-1 effectively inhibits G6PD. Across a range of cultured cells, G6PDi-1 depletes NADPH most strongly in lymphocytes. In T cells but not macrophages, G6PDi-1 markedly decreases inflammatory cytokine production. In neutrophils, it suppresses respiratory burst.[1]
Cell Research Cell lines HepG2 cells, HCT116 cells, T cells
Concentrations 25 μM, 50 μM, 75 μM, 100 μM
Incubation Time 2 h, 4 h, 6 h
Method

To analyze intracellular cytokines, active T cells are re-stimulated with PMA (20 ng/ml) and ionomycin (1 ug/ml) in the presence of GolgiStop and increasing concentrations of G6PDi-1. After a 6h incubation period, cells are collected, washed with PBS and stained with the viability dye Live/Dead Aqua.
For measuring intracellular ROS levels, cells are incubated for 2 h in the presence of increasing concentrations of G6PDi-1 and then stained for ROS and viability using CellROX Green Flow Cytometry Assay Kit following manufacturer instructions.

Chemical Information & Solubility

Molecular Weight 284.34 Formula

C14H12N4OS
CAS No. 2457232-14-1 SDF --
Smiles O=C1CCCCC2=C1C=NC(=N2)NC3=CSC(=C3)C#N
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 57 mg/mL ( (200.46 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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