G6PDi-1

Catalog No.S9617 Batch:S961701

Technical Data

Formula	C₁₄H₁₂N₄OS
Molecular Weight	284.34	CAS No.	2457232-14-1
Solubility (25°C)*	In vitro	DMSO	57 mg/mL (200.46 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

G6PDi-1 is an effective inhibitor of glucose-6-phosphate dehydrogenase (G6PD). G6PDi-1 depletes NADPH and decreases inflammatory cytokine production.

Targets

G6PD ^[1]

NADPH ^[1]

In vitro

G6PDi-1 effectively inhibits G6PD. Across a range of cultured cells, G6PDi-1 depletes NADPH most strongly in lymphocytes. In T cells but not macrophages, G6PDi-1 markedly decreases inflammatory cytokine production. In neutrophils, it suppresses respiratory burst.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HepG2 cells, HCT116 cells, T cells Concentrations 25 μM, 50 μM, 75 μM, 100 μM Incubation Time 2 h, 4 h, 6 h Method To analyze intracellular cytokines, active T cells are re-stimulated with PMA (20 ng/ml) and ionomycin (1 ug/ml) in the presence of GolgiStop and increasing concentrations of G6PDi-1. After a 6h incubation period, cells are collected, washed with PBS and stained with the viability dye Live/Dead Aqua. For measuring intracellular ROS levels, cells are incubated for 2 h in the presence of increasing concentrations of G6PDi-1 and then stained for ROS and viability using CellROX Green Flow Cytometry Assay Kit following manufacturer instructions.

Cell Assay:^{^[1]}

Cell lines
HepG2 cells, HCT116 cells, T cells
Concentrations
25 μM, 50 μM, 75 μM, 100 μM
Incubation Time
2 h, 4 h, 6 h
Method
To analyze intracellular cytokines, active T cells are re-stimulated with PMA (20 ng/ml) and ionomycin (1 ug/ml) in the presence of GolgiStop and increasing concentrations of G6PDi-1. After a 6h incubation period, cells are collected, washed with PBS and stained with the viability dye Live/Dead Aqua.
For measuring intracellular ROS levels, cells are incubated for 2 h in the presence of increasing concentrations of G6PDi-1 and then stained for ROS and viability using CellROX Green Flow Cytometry Assay Kit following manufacturer instructions.

References

[1] Jonathan M Ghergurovich, et al. Nat Chem Biol. 2020 Jul;16(7):731-739.

Selleck's G6PDi-1 has been cited by 1 publication

IDH2 stabilizes HIF-1α-induced metabolic reprogramming and promotes chemoresistance in urothelial cancer [ EMBO J, 2023, e110620.]	PubMed: 36637036

IDH2 stabilizes HIF-1α-induced metabolic reprogramming and promotes chemoresistance in urothelial cancer [ EMBO J, 2023, e110620.]

PubMed: 36637036

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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