6AN (6-Aminonicotinamide)

Catalog No.S9783 Batch:S978302

Technical Data

Formula	C₆H₇N₃O
Molecular Weight	137.14	CAS No.	329-89-5
Solubility (25°C)*	In vitro	DMSO	27 mg/mL (196.87 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

6AN (6-Aminonicotinamide) is an antimetabolite used to inhibit the NADPH-producing pentose phosphate pathway (PPP) in many cellular systems, making them more susceptible to oxidative stress. 6-Aminonicotinamide is a competitive inhibitor of NADP+-dependent enzyme glucose-6-phosphate dehydrogenase (G6PD) with Ki of 0.46 μM.

Targets

G6PD ^[1]
(Cell-free assay)

0.46 μM(Ki)

In vitro

Blocking the pentose phosphate pathway (PPP) by 6-AN significantly inhibits mES colony formation in the presence of a normal concentration of glucose. 6-AN treatment effectively inhibits AKT phosphorylation and activation of its downstream effector S6. 6-AN treatment has no effect on the ERK and PDK1 activities, suggesting an AKT-specific effect. 6-AN treatment inhibits cell growth.^[2]

In vivo

PTEN null human cancer cells and in vivo murine models are sensitive to 6-AN (anti-PPP, anti pentose phosphate pathway) treatments, suggesting the importance of the PPP in maintaining AKT activation even in the presence of a constitutively activated PI3K pathway. The treatment of TALL models with 6-AN significantly increases the Phlda3 mRNA and protein levels, accompanied by substantial decreases in the levels of P-AKT and P-S6 as well as the PPP metabolic intermediates.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines HeLa cells, mES cells Concentrations 100 nM Incubation Time 24 h, 2 days, 3 days, 4 days Method PHLDA3 knockout blocks the effects of 6-AN, R5P or uridine on cell growth in vitro. Isogenic PHLDA3 WT and knockout HeLa cells are seeded in 96-well plates at a density of 1000 cells/well. After 48 h, the cells are treated with 6-AN (100 nM). Cell viability is detected by CCK-8 assays at indicated time points.
Animal Study:^{^[2]}	Animal Models male CAnN.Cg-Foxn1nu/CrlVr mice Dosages 1 mg/kg, 15 mg/kg Administration IP

Cell Assay:^{^[2]}

Cell lines
HeLa cells, mES cells
Concentrations
100 nM
Incubation Time
24 h, 2 days, 3 days, 4 days
Method
PHLDA3 knockout blocks the effects of 6-AN, R5P or uridine on cell growth in vitro. Isogenic PHLDA3 WT and knockout HeLa cells are seeded in 96-well plates at a density of 1000 cells/well. After 48 h, the cells are treated with 6-AN (100 nM). Cell viability is detected by CCK-8 assays at indicated time points.

Animal Study:^{^[2]}

Animal Models
male CAnN.Cg-Foxn1nu/CrlVr mice
Dosages
1 mg/kg, 15 mg/kg
Administration
IP

References

Selleck's 6AN (6-Aminonicotinamide) has been cited by 10 publications

Therapeutic modulation of ROCK overcomes metabolic adaptation of cancer cells to OXPHOS inhibition and drives synergistic anti-tumor activity [ bioRxiv, 2024, 2024.09.16.613317]	PubMed: 39345502
Metabolic classification suggests the GLUT1/ALDOB/G6PD axis as a therapeutic target in chemotherapy-resistant pancreatic cancer [ Cell Rep Med, 2023, S2666-3791(23)00315-4]	PubMed: 37597521
Metabolic classification suggests the GLUT1/ALDOB/G6PD axis as a therapeutic target in chemotherapy-resistant pancreatic cancer [ Cell Rep Med, 2023, 4(9):101162]	PubMed: 37597521
PI3K/mTOR inhibitors promote G6PD autophagic degradation and exacerbate oxidative stress damage to radiosensitize small cell lung cancer [ Cell Death Dis, 2023, 14(10):652]	PubMed: 37802999
PI3K/mTOR inhibitors promote G6PD autophagic degradation and exacerbate oxidative stress damage to radiosensitize small cell lung cancer [ Cell Death Dis, 2023, 14(10):652]	PubMed: 37802999
Aberrant metabolite trafficking and fuel sensitivity in human pluripotent stem cell-derived islets [ Cell Rep, 2023, 42(8):112970]	PubMed: 37556323
Insulin-induced gene 2 protects against hepatic ischemia-reperfusion injury via metabolic remodeling [ J Transl Med, 2023, 21(1):739]	PubMed: 37858181
Insulin-induced gene 2 protects against hepatic ischemia-reperfusion injury via metabolic remodeling [ J Transl Med, 2023, 21(1):739]	PubMed: 37858181
Metabolic heterogeneity protects metastatic mucosal melanomas cells from ferroptosis [ Int J Mol Med, 2022, 50(4)124]	PubMed: 36004461
Metabolic plasticity imparts erlotinib-resistance in pancreatic cancer by upregulating glucose-6-phosphate dehydrogenase [ Cancer Metab, 2020, 8:19]	PubMed: 32974013

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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