Home Metabolism Dehydrogenase inhibitor 3-Nitropropionic acid (3-NP) For research use only.

3-Nitropropionic acid (3-NP)

Synonyms: β-Nitropropionic Acid

3-Nitropropionic acid (3-NP) is an irreversible inhibitor of mitochondrial respiratory Complex II succinate dehydrogenase, resulting in energy depletion through disruption of the electron transport chain.

3-Nitropropionic acid (3-NP) Chemical Structure

3-Nitropropionic acid (3-NP) Chemical Structure

CAS No. 504-88-1

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3-Nitropropionic acid (3-NP) Related Products

Signaling Pathway

Dehydrogenase Signaling Pathways

Dehydrogenase Signaling Pathway

Biological Activity

Description 3-Nitropropionic acid (3-NP) is an irreversible inhibitor of mitochondrial respiratory Complex II succinate dehydrogenase, resulting in energy depletion through disruption of the electron transport chain.
Targets
Succinate dehydrogenase [1]
In vitro
In vitro 3-nitropropionic acid (3-NPA) is an irreversible inhibitor of complex II in the mitochondria. It impairs cellular energy metabolism via inhibition of succinate dehydrogenase, which induces a reduction in ATP production and leads to oxidative stress. 3-NPA can cause the generation and release of ROS from mitochondria, mitochondrial DNA damage, and thus loss of mitochondrial function. 3-NPA reduces the number of large follicles, impairs oocyte development, and increases the percentage of atretic large follicles and ROS levels in oocytes and granulosa cells[2]. ROS production followed by mitochondrial DNA damage is the primary event in 3-NPA toxicity, and Bcl-2 protects PC12 cells from 3-NPA toxicity by preventing mitochondrial DNA damage[3].
Cell Research Cell lines PC12 cells
Concentrations 4-8 mM
Incubation Time 48 h
Method Viability of the PC12 cells with or without treatment of 3-NPA is estimated using trypan blue exclusion test. Cells are grown in Petri dishes to a density of 1 × 106 cells/dish. After treatment with 4 or 8 mM 3-NPA for 48 h, cells are washed with Hank's balanced salt solution. Trypan blue (0.4 %) is added to the cells and incubated for 5 min. Both viable and nonviable cells are scored on a hemocytometer using a Nikon microscope. The nonviable cells are expressed as a percentage of total cell population
In Vivo
In vivo 3-NPA induces granulosa cell apoptosis, large follicle atresia, and an increase of ROS levels in the ovary[2].
Animal Research Animal Models ICR mice
Dosages 6.25, 12.5 and 25 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 119.08 Formula

C3H5NO4
CAS No. 504-88-1 SDF Download 3-Nitropropionic acid (3-NP) SDF
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 23 mg/mL ( (193.14 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 23 mg/mL

Ethanol : 23 mg/mL


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In vivo
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