3-Nitropropionic acid (3-NP)

Catalog No.S3652 Batch:S365203

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Technical Data

Formula

C3H5NO4
Molecular Weight 119.08 CAS No. 504-88-1
Solubility (25°C)* In vitro DMSO 24 mg/mL (201.54 mM)
Water 24 mg/mL (201.54 mM)
Ethanol 24 mg/mL (201.54 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description 3-Nitropropionic acid (3-NP) is an irreversible inhibitor of mitochondrial respiratory Complex II succinate dehydrogenase, resulting in energy depletion through disruption of the electron transport chain.
Targets
Succinate dehydrogenase [1]
In vitro 3-nitropropionic acid (3-NPA) is an irreversible inhibitor of complex II in the mitochondria. It impairs cellular energy metabolism via inhibition of succinate dehydrogenase, which induces a reduction in ATP production and leads to oxidative stress. 3-NPA can cause the generation and release of ROS from mitochondria, mitochondrial DNA damage, and thus loss of mitochondrial function. 3-NPA reduces the number of large follicles, impairs oocyte development, and increases the percentage of atretic large follicles and ROS levels in oocytes and granulosa cells[2]. ROS production followed by mitochondrial DNA damage is the primary event in 3-NPA toxicity, and Bcl-2 protects PC12 cells from 3-NPA toxicity by preventing mitochondrial DNA damage[3].
In vivo 3-NPA induces granulosa cell apoptosis, large follicle atresia, and an increase of ROS levels in the ovary[2].

Protocol (from reference)

Cell Assay:[3]
  • Cell lines

    PC12 cells

  • Concentrations

    4-8 mM

  • Incubation Time

    48 h

  • Method

    Viability of the PC12 cells with or without treatment of 3-NPA is estimated using trypan blue exclusion test. Cells are grown in Petri dishes to a density of 1 × 106 cells/dish. After treatment with 4 or 8 mM 3-NPA for 48 h, cells are washed with Hank's balanced salt solution. Trypan blue (0.4 %) is added to the cells and incubated for 5 min. Both viable and nonviable cells are scored on a hemocytometer using a Nikon microscope. The nonviable cells are expressed as a percentage of total cell population
Animal Study:[2]
  • Animal Models

    ICR mice

  • Dosages

    6.25, 12.5 and 25 mg/kg

  • Administration

    i.p.

Selleck's 3-Nitropropionic acid (3-NP) has been cited by 2 publications

Mitochondrial fumarate promotes ischemia/reperfusion-induced tubular injury [ Acta Physiol (Oxf), 2024, 240(4):e14121.] PubMed: 38409944
Arachidonic Acid Metabolism Controls Macrophage Alternative Activation Through Regulating Oxidative Phosphorylation in PPARγ Dependent Manner [ Front Immunol, 2021, 12:618501] PubMed: 34149684

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.