Home DNA Damage/DNA Repair DNA/RNA Synthesis inhibitor Procarbazine HCl For research use only.

Procarbazine HCl

Synonyms: NSC-77213 HCl

Procarbazine HCl (NSC-77213) is a hydrochloride salt form of procarbazine which is a polyfunctional alkylating compound, used for the treatment of Hodgkin's lymphoma.

Procarbazine HCl Chemical Structure

Procarbazine HCl Chemical Structure

CAS No. 366-70-1

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Procarbazine HCl Related Products

Signaling Pathway

DNA/RNA Synthesis Signaling Pathways

DNA/RNA Synthesis Signaling Pathway

Biological Activity

Description Procarbazine HCl (NSC-77213) is a hydrochloride salt form of procarbazine which is a polyfunctional alkylating compound, used for the treatment of Hodgkin's lymphoma.
In vitro
In vitro

Procarbazine plus Cu(II) induce piperidine-labile and formamidopyrimidine-DNA glycosylase-sensitive lesions at the 5'-ACG-3' sequence, complementary to a hotspot of the p53 gene, and the 5'-TG-3' sequence. Procarbazine causes DNA damage through non-enzymatic formation of the Cu(I)-hydroperoxo complex and methyl radicals. [1] Procarbazine has a strong clastogenic effect in hematopoietic cells and is mutagenic in a variety organs after high dose treatment. [2]
In Vivo
In vivo

Procarbazine causes significant decrease in testicular and epididymal weight and a drastic reduction in haploid cells and spermatogenic arrest, demonstrating variation among the test golden hamster. [3] Procarbazine produces a dose-dependent potentiation of MAO A in brown adipose tissue, the elevation being more pronounced following monomethylhydrazine, with activity rising to 350% of that in control homogenates in rats. Procarbazine or monomethylhydrazine reduces metabolism of this amine by a similar degree as had been determined ex-vivo in blood vessel homogenates. [4] Procarbazine is mutagenic, clastogenic and teratogenic in a wide range of test systems of varying complexity and a wide-spectrum carcinogen in rodents and monkeys, causing tumours of the haemopoietic system, the mammary gland, the lung and the nervous system. Procarbazine in vivo undergoes a complex series of metabolic changes that result in the generation of a number of chemically reactive species, including methylating agents and free radicals. [5]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00003564 Withdrawn
Brain and Central Nervous System Tumors
M.D. Anderson Cancer Center|National Cancer Institute (NCI)
 Phase 3

Chemical Information & Solubility

Molecular Weight 257.76 Formula

C12H19N3O.HCl
CAS No. 366-70-1 SDF Download Procarbazine HCl SDF
Smiles CC(C)NC(=O)C1=CC=C(C=C1)CNNC.Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 51 mg/mL ( (197.85 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 51 mg/mL

Ethanol : 34 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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