Home Cell Cycle CDK inhibitor Bohemine For research use only.

Bohemine

Bohemine is a CDK inhibitor with IC50s of 4.6, 83, and 2.7 μM for Cdk2/cyclin E, Cdk2/cyclin A, and Cdk9/cyclin T1, respectively.

Bohemine Chemical Structure

Bohemine Chemical Structure

CAS No. 189232-42-6

Purity & Quality Control

Bohemine Related Products

Signaling Pathway

CDK Signaling Pathways

CDK Signaling Pathway

Biological Activity

Description Bohemine is a CDK inhibitor with IC50s of 4.6, 83, and 2.7 μM for Cdk2/cyclin E, Cdk2/cyclin A, and Cdk9/cyclin T1, respectively.
Targets
Cdk9/cyclin T1 [1] Cdk2/cyclin E [1] Cdk2/cyclin A [1]
2.7 μM 4.6 μM 83 μM
In vitro
In vitro

Treatment of HCT116 cells bohemine results in a 11% to 15% loss of cells from the S phase of the cell cycle with a 6% to 17% increase in the proportion of cells in the G2-M phase after 24 hours of treatment, as determined by propidium iodide staining and analysis by flow cytometry.Bohemine reduces RB phosphorylation at pharmacologically relevant concentrations that inhibit cell growth in cell culture[1].
In Vivo
In vivo

Given i.v., the single dose maximum tolerated dose for bohemine is 100 mg/kg. Following administration of 50 mg/kg i.v. bohemine to BALB/c− mice, Cmax is 72,308 nM, observed clearance is 0.23 L/h and T1/2 is 1.39 h[1].
Animal Research Animal Models BALB/c-mice bearing the s.c. colon 26 murine tumor
Dosages 50 mg/kg
Administration IV

Chemical Information & Solubility

Molecular Weight 340.42 Formula

C18H24N6O
CAS No. 189232-42-6 SDF --
Smiles CC(C)N1C=NC2=C(N=C(N=C21)NCCCO)NCC3=CC=CC=C3
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 68 mg/mL ( (199.75 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 68 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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