Home Apoptosis Caspase inhibitor Z-YVAD-FMK For research use only.

Z-YVAD-FMK

Z-YVAD-FMK is a cell-permeable and irreversible caspase-1 specific inhibitor with anti-inflammatory and anti-tumor activities.

Z-YVAD-FMK Chemical Structure

Z-YVAD-FMK Chemical Structure

CAS No. 210344-97-1

Purity & Quality Control

Z-YVAD-FMK Related Products

Signaling Pathway

Caspase Signaling Pathways

Caspase Signaling Pathway

Biological Activity

Description Z-YVAD-FMK is a cell-permeable and irreversible caspase-1 specific inhibitor with anti-inflammatory and anti-tumor activities.
Targets
caspase-1 [1]
In vitro
In vitro

Z-YVAD-FMK treatment significantly reduces the increased caspase-1 activity induced by LPS+Aβ. Z-YVAD-FMK remarkably suppresses the protein expressions of cleaved IL-1β, cleaved IL-18, and Iba-1.[1]
Cell Research Cell lines BV2 cells
Concentrations 5 μM
Incubation Time 1 h
Method

BV2 cells are treated without or with EGCG (10 μM) or/and Z-YVAD-FMK (5 μM) for 1 h, followed by stimulationwith LPS (1 μg/mL) for 1 h and Aβ1-42 (10 μM) for 6 h. The cells are harvested at room temperature, and the protein concentration is determined by the Bradfordmethod. A calibration curve is constructed, and caspase-1 activity is calculated according to it.
In Vivo
In vivo

Z-YVAD-FMK is a cell-permeable caspase-6 inhibitor.
Animal Research Animal Models C57BL/6J mice
Dosages 50 μM
Administration i.v.

Chemical Information & Solubility

Molecular Weight 630.66 Formula

C31H39FN4O9
CAS No. 210344-97-1 SDF --
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (158.56 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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