Home Apoptosis Caspase inhibitor Z-IETD-FMK For research use only.

Z-IETD-FMK

Synonyms: Caspase-8 Inhibitor, Z-IE(OMe)TD(OMe)-FMK

Z-IETD-FMK (Caspase-8 Inhibitor, Z-IE(OMe)TD(OMe)-FMK) is a specific Caspase-8 inhibitor. Z-IETD-FMK is also an inhibitor of granzyme B.

Z-IETD-FMK Chemical Structure

Z-IETD-FMK Chemical Structure

CAS No. 210344-98-2

Purity & Quality Control

Products often used together with Z-IETD-FMK

Z-LEHD-FMK TFA

Z-IETD-FMK and Z-LEHD-FMK TFA are used to assess if caspase-8 and 9 affect the pro-survival actions of melatonin under hypoxia conditions in granuloma cells.

Tao JL, et al. Antioxidants (Basel). 2021 Jan 28;10(2):184.

Nimesulide

Z-IETD-FMK suppresses Nimesulide and radiation-induced cleavage activation of caspase-9/3/8 and Bid in A549 cells.

Kim BM, et al. Int J Oncol. 2009 May;34(5):1467-73.

Z-VAD-FMK

Z-IETD-FMK and Z-VAD-FMK pretreatment significantly attenuates 23-HUA-induced DNA fragmentation in HL-60 cells.

Won JH, et al. Molecules. 2018 Dec 13;23(12):3306.

Q-VD-Oph

Q-VD-Oph effectively promotes viral replication than Z-IETD-FMK in DEF cells.

Zhao C, et al. Viruses. 2019 Feb 24;11(2):196.

Z-IETD-FMK Related Products

Signaling Pathway

Caspase Signaling Pathways

Caspase Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2 Function assay Z-IETD-FMK accelerates the PPI-induced cell death of HepG2 cells. 30671458
HL-60 Function assay 50 μM 1 h completely blocks the 23-HUA-induced DNA fragmentation 30551620
MG63 Cytotoxicity assay 10 μM partially reversed MSP-4-induced MG63 cell cytotoxicity 29301308
HXO-RB44 cells Apoptosis assay decreases the apoptotic rates induced by bufalin 27904697
Jurkat cells Function assay 50 μM 1 h abolishes the cleavage of tBid 25632401
DU 145 Function assay 20 μM 24 h interrupted the cleavage of PARP induced by ergosterol peroxide 25506265
Click to View More Cell Line Experimental Data

Biological Activity

Description Z-IETD-FMK (Caspase-8 Inhibitor, Z-IE(OMe)TD(OMe)-FMK) is a specific Caspase-8 inhibitor. Z-IETD-FMK is also an inhibitor of granzyme B.
Targets
Caspase-8 [1]
In vitro
In vitro

Z-IETD-FMK, which inhibits the cleavage of caspase-8 and partially inhibits the cleavage of caspase-3 and PARP, prevents the execution of apoptosis in retinal cells exposed to different apoptotic stimuli. [1]

Z-IETD-FMK (50 μM) reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3. [2]

Inhibition of caspase-8 by Z-IETD-FMK affects the generation of activated/memory T cells and T cell cytokine production, and decreases NF-kappaB responses to TCR:CD3 engagement in T cell cultures. [3]
Cell Research Cell lines Rat neonatal cardiomyocytes
Concentrations 50 µM
Incubation Time 24 h
Method

Drugs were added to the cell culture medium simultaneously with ceramide, and surviving cells were counted 24 hours later.
In Vivo
In vivo

In vivo, inhibition of caspase-8 by Z-IETD-FMK reduces memory/activated CD4 and CD8 T cells, and increases susceptibility to T. cruzi infection. [3]

Z-IETD-FMK promotes neuronal survival and regeneration of injured retinal ganglion cells after CNS injuries. [4]
Animal Research Animal Models T. cruzi-infected mice
Dosages 0.4 mg/3 days
Administration --

Chemical Information & Solubility

Molecular Weight 654.68 Formula

C30H43FN4O11
CAS No. 210344-98-2 SDF Download Z-IETD-FMK SDF
Smiles CCC(C)C(C(=O)NC(CCC(=O)OC)C(=O)NC(C(C)O)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)OCC1=CC=CC=C1
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (152.74 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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