Home Apoptosis Caspase inhibitor Z-DEVD-FMK For research use only.

Z-DEVD-FMK

Synonyms: Caspase-3 Inhibitor

Z-DEVD-FMK (Caspase-3 Inhibitor) is a specific, irreversible Caspase-3 inhibitor, and also shows potent inhibition on caspase-6, caspase-7, caspase-8, and caspase-10.

Z-DEVD-FMK Chemical Structure

Z-DEVD-FMK Chemical Structure

CAS No. 210344-95-9

Purity & Quality Control

Z-DEVD-FMK Related Products

Signaling Pathway

Caspase Signaling Pathways

Caspase Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
duck embryo fibroblasts (DEFs) Function assay 2 h  effectively promoted viral replication 30813500
OSCC cell Apoptosis assay 20 µM 48 h reduce the activity of caspase 3 and caspase 9 30573978
A549 Function assay 25 μM 24 h significantly inhibit ROS generation in A549 cells 30240709
HCT116 cells Function assay 50 μM 2 h cotreatment with Xylopine to prevente the xylopine-induced increasing apoptosis 29362667
K562 cells Function assay 2 h increased R. verniciflua extract-induced cell proliferation 29328387
THP-1 cells Function assay 50 μM 2 h sub-G1 peak Inhibition in DMQA induced cell death 29225136
Click to View More Cell Line Experimental Data

Biological Activity

Description Z-DEVD-FMK (Caspase-3 Inhibitor) is a specific, irreversible Caspase-3 inhibitor, and also shows potent inhibition on caspase-6, caspase-7, caspase-8, and caspase-10.
Targets
Caspase-3 [1]
In vitro
In vitro Z-DEVD-FMK (1–200 μM) inhibits D4-GDI cleavage and apoptosis in a concentration-dependent manner. [1] Z-DEVD-FMK reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3. [3] Z-DEVD-FMK (100μM) attenuates OxyHb-induced cell detachment, reduced caspase-2 and -3 activities, abolishes OxyHb-induced DNA ladders, and prevents OxyHb-induced cleavage of PARP in cultured brain microvessel endothelial cells. [4] Z-DEVD-FMK (100 μM) blocks MPP+-induced increases in caspase-3 enzyme activity. Z-DEVD-FMK dose dependently blocks 6-OHDA-induced apoptotic cell death with IC50 of 18 μM. [5]
Kinase Assay Caspase activity assay
Caspase-3 and caspase-9 activities are measured using fluorescent-based substrate. After treatment, the cells are resuspended in lysis buffer (50 mM Tris HCl, 1 mM EDTA, and 10 mM EGTA) containing 10 mM digitonin for 20 min at 37°C. Supernatants are treated with either of the fluorogenic substrates Ac-DEVD-AFC for caspase-3 or Ac-LEHD-AFC for caspase-9 for 1 h at 37°C and fluorescence is measured at excitation at 400 nm and emission at 505 nm using a Gemini XS fluorescence plate reade
Cell Research Cell lines N27 cells
Concentrations ~50 μM
Incubation Time 24 hours
Method N27 cells are incubated with 100 μM 6-OHDA for 24 h or 300 μM MPP+ for 36 h in the presence or absence of 50 μM Z-DEVD-FMK and cell death is determined by MTT (3-(4,5-dimethylthiazol-3-yl)-2,5-diphenyl tetrazolium bromide) assay, which is widely used to assess cell viability. After treatment, the cells are incubated in serum-free medium containing 0.25 mg/ml MTT for 3 h at 37°C. Formation of formazan from tetrazolium is measured at 570 nm with a reference wavelength at 630 nm using a SpectraMax microplate reader.
Experimental Result Images Methods Biomarkers Images PMID
Western blot HAUSP / Kinesin 5B / GEP100 / SDCCAG3 / PARD3 / Lamin A/C / PARP CLK3 / ADH4 / MDH1 ORF57 / caspase 7 / caspase 3 24195789
In Vivo
In vivo Z-DEVD-FMK, before and after injury, markedly reduces post-traumatic apoptosis, and significantly improved neurological recovery. [2]
Animal Research Animal Models Male Sprague Dawley rats with Brain trauma.
Dosages 160 ng
Administration Intracerebroventricular administration

Chemical Information & Solubility

Molecular Weight 668.66 Formula

C30H41FN4O12
CAS No. 210344-95-9 SDF Download Z-DEVD-FMK SDF
Smiles CC(C)C(C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)C(CCC(=O)OC)NC(=O)C(CC(=O)OC)NC(=O)OCC1=CC=CC=C1
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (149.55 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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