Z-YVAD-FMK

Catalog No.S8507 Batch:S850702

Technical Data

Formula	C₃₁H₃₉FN₄O₉
Molecular Weight	630.66	CAS No.	210344-97-1
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (158.56 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Z-YVAD-FMK is a cell-permeable and irreversible caspase-1 specific inhibitor with anti-inflammatory and anti-tumor activities.

Targets

caspase-1 ^[1]

In vitro

Z-YVAD-FMK treatment significantly reduces the increased caspase-1 activity induced by LPS+Aβ. Z-YVAD-FMK remarkably suppresses the protein expressions of cleaved IL-1β, cleaved IL-18, and Iba-1.^[1]

In vivo

Z-YVAD-FMK is a cell-permeable caspase-6 inhibitor.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines BV2 cells Concentrations 5 μM Incubation Time 1 h Method BV2 cells are treated without or with EGCG (10 μM) or/and Z-YVAD-FMK (5 μM) for 1 h, followed by stimulationwith LPS (1 μg/mL) for 1 h and Aβ1-42 (10 μM) for 6 h. The cells are harvested at room temperature, and the protein concentration is determined by the Bradfordmethod. A calibration curve is constructed, and caspase-1 activity is calculated according to it.
Animal Study:^{^[3]}	Animal Models C57BL/6J mice Dosages 50 μM Administration i.v.

Cell Assay:^{^[1]}

Cell lines
BV2 cells
Concentrations
5 μM
Incubation Time
1 h
Method
BV2 cells are treated without or with EGCG (10 μM) or/and Z-YVAD-FMK (5 μM) for 1 h, followed by stimulationwith LPS (1 μg/mL) for 1 h and Aβ1-42 (10 μM) for 6 h. The cells are harvested at room temperature, and the protein concentration is determined by the Bradfordmethod. A calibration curve is constructed, and caspase-1 activity is calculated according to it.

Animal Study:^{^[3]}

Animal Models
C57BL/6J mice
Dosages
50 μM
Administration
i.v.

References

Selleck's Z-YVAD-FMK has been cited by 9 publications

Melatonin alleviates chronic stress-induced hippocampal microglia pyroptosis and subsequent depression-like behaviors by inhibiting Cathepsin B/NLRP3 signaling pathway in rats [ Transl Psychiatry, 2024, 14(1):166]	PubMed: 38538614
ER stress induces caspase-2-tBID-GSDME-dependent cell death in neurons lytically infected with herpes simplex virus type 2 [ EMBO J, 2023, e113118.]	PubMed: 37646198
Mannose-doped metal-organic frameworks induce tumor cell pyroptosis via the PERK pathway [ J Nanobiotechnology, 2023, 21(1):426]	PubMed: 37968665
Toxoplasma gondii Rhoptry Protein 7 (ROP7) Interacts with NLRP3 and Promotes Inflammasome Hyperactivation in THP-1-Derived Macrophages [ Cells, 2022, 11(10)1630]	PubMed: 35626667
Downregulated XBP-1 Rescues Cerebral Ischemia/Reperfusion Injury-Induced Pyroptosis via the NLRP3/Caspase-1/GSDMD Axis [ Mediators Inflamm, 2022, 2022:8007078]	PubMed: 35497095
Downregulated XBP-1 Rescues Cerebral Ischemia/Reperfusion Injury-Induced Pyroptosis via the NLRP3/Caspase-1/GSDMD Axis [ Mediators Inflamm, 2022, 2022:8007078]	PubMed: 35497095
Gain-of-function hot spot mutant p53R248Q regulation of integrin/FAK/ERK signaling in esophageal squamous cell carcinoma [ Transl Oncol, 2021, 14(1):100982]	PubMed: 33395748
Cepharanthine attenuates cerebral ischemia/reperfusion injury by reducing NLRP3 inflammasome-induced inflammation and oxidative stress via inhibiting 12/15-LOX signaling [ Biomed Pharmacother, 2020, 127:110151]	PubMed: 32559840
Propofol Attenuates Inflammatory Damage via Inhibiting NLRP1-Casp1-Casp6 Signaling in Ischemic Brain Injury [ Biol Pharm Bull, 2020, 43(10):1481-1489]	PubMed: 32999158

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.