Home Epigenetics HDAC inhibitor Splitomicin For research use only.

Splitomicin

Splitomicin is a selective NAD(+)-dependent histone deacetylase Sir2p inhibitor with IC50 of 60 μM, showing a higher activity in a cell-based assay.

Splitomicin Chemical Structure

Splitomicin Chemical Structure

CAS No. 5690-03-9

Purity & Quality Control

Batch: S759301 DMSO]39 mg/mL]false]Ethanol]39 mg/mL]false]Water]Insoluble]false Purity: 99.45%
99.45

Splitomicin Related Products

Signaling Pathway

HDAC Signaling Pathways

HDAC Signaling Pathway

Biological Activity

Description Splitomicin is a selective NAD(+)-dependent histone deacetylase Sir2p inhibitor with IC50 of 60 μM, showing a higher activity in a cell-based assay.
Targets
Sir2p [1]
60 μM
In vitro
In vitro Splitomicin inhibits Sir2p deacetylase activity by altering or blocking access to the acetylated histone binding pocket. [1] Splitomicin inhibits platelet aggregation induced by thrombin, collagen, AA and U46619 via inhibition of cyclic AMP phosphodiesterase activity and subsequent inhibition of intracellular Ca(++) mobilization, TXB2 formation and ATP release. [2]
In Vivo
In vivo In a photochemical injury mouse model, splitomicin (80 mg/kg/d i.p.) enhances tissue factor activity in the arterial vessel wall and promotes carotid artery thrombus formation. [3]
Animal Research Animal Models Carotid artery thrombosis mouse model
Dosages 80 mg/kg/d
Administration i.p.

Chemical Information & Solubility

Molecular Weight 198.22 Formula

C13H10O2
CAS No. 5690-03-9 SDF Download Splitomicin SDF
Smiles C1CC(=O)OC2=C1C3=CC=CC=C3C=C2
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 39 mg/mL ( (196.75 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 39 mg/mL

Water : Insoluble


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In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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