Home Epigenetics HDAC inhibitor - Autophagy activator - Apoptosis related activator Sodium butyrate For research use only.

Sodium butyrate

Synonyms: NaB, Butanoic acid sodium salt

Sodium butyrate (NaB, Butanoic acid sodium salt), sodium salt of butyric acid, is a histone deacetylase inhibitor and competitively binds to the zinc sites of class I and II histone deacetylases (HDACs). Sodium butyrate (NaB) inhibits cell cycle progression, promotes differentiation, and induces apoptosis and autophagy in several types of cancer cells.

Sodium butyrate Chemical Structure

Sodium butyrate Chemical Structure

CAS No. 156-54-7

Purity & Quality Control

Sodium butyrate Related Products

Signaling Pathway

HDAC Signaling Pathways

HDAC Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human K562 cells Proliferation assay 1 mM Antiproliferative activity against human K562 cells at 1 mM in presence of guanosine 23937979
human H1299 cells Function assay 7 days Inhibition of human USP1/UAF1 in human H1299 cells assessed as reduction in colony formation after 7 days, EC50=3 μM 25229643
human JEG3 cells Function assay Inhibition of aromatase in human JEG3 cells by scintillation spectrometry, IC50=0.0015 μM 20148564
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Biological Activity

Description Sodium butyrate (NaB, Butanoic acid sodium salt), sodium salt of butyric acid, is a histone deacetylase inhibitor and competitively binds to the zinc sites of class I and II histone deacetylases (HDACs). Sodium butyrate (NaB) inhibits cell cycle progression, promotes differentiation, and induces apoptosis and autophagy in several types of cancer cells.
Targets
Histone deacetylase [6]
In vitro
In vitro Butyrate mediates growth inhibition of colonic cancer cells and thereby to elucidate the molecular link between a high-fiber diet and the arrest of colon carcinogenesis. Butyrate induces p21 mRNA expression in an immediate-early fashion, through transactivation of a promoter cis-element(s) located within 1.4 kb of the transcriptional start site, independent of p53 binding. [1] Sodium butyrate, at physiological concentrations, induces apoptosis in 2 adenoma cell lines, RG/C2 and AA/Cl, and in the carcinoma cell line PC/JW/FI. [2] Butyrate exerts potent effects on a variety of colonic mucosal functions such as inhibition of inflammation and carcinogenesis, reinforcing various components of the colonic defence barrier and decreasing oxidative stress. [3] Butyrate inhibits most HDAC except class III HDAC and class II HDAC6 and -10. Butyrate also is the most effective fatty acid in stimulating or repressing the expression of specific genes. [4]
Experimental Result Images Methods Biomarkers Images PMID
Western blot Cyclin D3 / CDK4 / CDK6 / CDK2 p15INK4b / p21Cip1 Phospho-H3 Ser10 / Acetyl-H3Lys9 / Acetyl-H3Lys14 / Dimethyl-H3 24280698
Immunofluorescence Acetyl-H3Lys9 24280698
In Vivo
In vivo Sodium butyrate significantly extends survival in a dose-dependent manner, improves body weight and motor performance, and delays the neuropathological sequelae in the R6/2 transgenic mouse model of Huntington's disease (HD). Sodium butyrate also increases histone and specificity protein-1 acetylation and protects against 3-nitropropionic acid neurotoxicity. [5]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00800930 Completed
Shigellosis
International Centre for Diarrhoeal Disease Research Bangladesh|Swedish International Development Cooperation Agency (SIDA)|Karolinska Institutet
 January 2005 Phase 2

Chemical Information & Solubility

Molecular Weight 110.09 Formula

C4H7NaO2
CAS No. 156-54-7 SDF Download Sodium butyrate SDF
Smiles CCCC(=O)[O-].[Na+]
Storage (From the date of receipt)

In vitro
Batch:

Water : 22 mg/mL

Ethanol : 8 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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