Home DNA Damage/DNA Repair HDAC inhibitor 4-PBA (Sodium Phenylbutyrate) For research use only.

4-PBA (Sodium Phenylbutyrate)

Synonyms: 4-Phenylbutyric acid, NaPB

4-PBA (Sodium Phenylbutyrate) is a salt of 4-phenylbutyrate (4-PBA) or 4-phenylbutyric acid.Sodium phenylbutyrate is a histone deacetylase inhibitor, used to treat urea cycle disorders.

4-PBA (Sodium Phenylbutyrate) Chemical Structure

4-PBA (Sodium Phenylbutyrate) Chemical Structure

CAS No. 1716-12-7

Purity & Quality Control

4-PBA (Sodium Phenylbutyrate) Related Products

Signaling Pathway

HDAC Signaling Pathways

HDAC Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Hela Function assay Inhibition of HDAC in human Hela cells nuclear extracts by fluorimetric assay, Ki=6.34μM 19520580
U937 Function assay 4 mM 8 to 48 hrs Induction of CAMP mRNA expression in human U937 cells at 4 mM after 8 to 48 hrs by RT-PCR analysis 19770273
VA10 Function assay 4 mM 24 hrs Induction of CAMP mRNA expression in human VA10 cells at 4 mM after 24 hrs by RT-PCR analysis 19770273
HT-29 Function assay 4 mM 8 to 48 hrs Induction of CAMP mRNA expression in human HT-29 cells at 4 mM after 8 to 48 hrs by RT-PCR analysis 19770273
A498 Function assay 4 mM 8 to 48 hrs Induction of CAMP mRNA expression in human A498 cells at 4 mM after 8 to 48 hrs by RT-PCR analysis 19770273
VA10 Function assay 4 mM 24 hrs Induction of DEFB1 mRNA expression in human VA10 cells at 4 mM after 24 hrs by RT-PCR analysis 19770273
VA10 Function assay 4 mM 24 hrs Induction of LL-37 protein expression in human VA10 cells at 4 mM after 24 hrs by Western blotting 19770273
VA10 Function assay 20 nM 24 hrs Induction of LL-37 protein expression in human VA10 cells at 20 nM after 24 hrs by Western blotting 19770273
Click to View More Cell Line Experimental Data

Biological Activity

Description 4-PBA (Sodium Phenylbutyrate) is a salt of 4-phenylbutyrate (4-PBA) or 4-phenylbutyric acid.Sodium phenylbutyrate is a histone deacetylase inhibitor, used to treat urea cycle disorders.
Targets
HDAC [1]
In vitro
In vitro

Phenylbutyrate is a well-known HDAC inhibitor, which increases gene transcription of a number of genes, and also exerts neuroprotective effects. Phenylbutyrate significantly attenuates MPTP-induced depletion of striatal dopamine and loss of tyrosine hydroxylase-positive neurons in the substantia nigra. [1]

Phenylbutyrate attenuates the expression of the apoptosis antagonist Bcl-X(L), the double-strand break repair protein DNA-dependent protein kinase, the prostate progression marker caveolin-1, and the pro-angiogenic vascular endothelial growth factor in prostate cancer cells. Phenylbutyrate is found to act in synergy with ionizing radiation to induce apoptosis in prostate cancer cells. [2]
Cell Research Cell lines HK-2 cells
Concentrations 1 mM
Incubation Time 24 h
Method

Cells were treated with indicated concentration of drug for 24 h.
Experimental Result Images Methods Biomarkers Images PMID
Western blot Survivin p38 / p-p38 / ERK / p-ERK / JNK / p-JNK 27274278
In Vivo
In vivo

Phenylbutyrate significantly extends survival and improved both the clinical and neuropathological phenotypes in G93A transgenic ALS mice. Phenylbutyrate administration ameliorates histone hypoacetylation observed in G93A mice and induced expression of nuclear factor-kappaB (NF-kappaB) p50, the phosphorylated inhibitory subunit of NF-kappaB (pIkappaB) and beta cell lymphoma 2 (bcl-2), but reduced cytochrome c and caspase expression. Phenylbutyrate acts to phosphorylate IkappaB, translocating NF-kappaB p50 to the nucleus, or to directly acetylate NF-kappaB p50. [3]

Phenylbutyrate increases brain histone acetylation and decreased histone methylation levels as assessed by both immunocytochemistry and Western blots in a transgenic mousemodel of Huntington's disease (HD). Phenylbutyrate increases mRNA for components of the ubiquitin-proteosomal pathway and down-regulated caspases implicated in apoptotic cell death, and active caspase 3 immunoreactivity in the striatum. [4]
Animal Research Animal Models Male C57BL/6 mice
Dosages 120 mg/kg
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05983588 Recruiting
Corticobasal Syndrome (CBS)
Technical University of Munich
 December 12 2023 Phase 2
NCT05019417 Unknown status
Monocarboxylate Transporter 8 Deficiency
Kaplan Medical Center|Weizmann Institute of Science
 June 30 2021 Phase 2|Phase 3
NCT04937062 Enrolling by invitation
STXBP1 Encephalopathy With Epilepsy SLC6A1 Neurodevelopmental Disorder|Developmental and Epileptic Encephalopathy
Weill Medical College of Cornell University|Children''s Hospital Colorado|SLC6A1 Connect|STXBP1 Foundation|Clara Inspired|University of Pennsylvania Orphan Disease Center|Horizon Therapeutics
 March 1 2021 Early Phase 1
NCT04421677 Completed
Inclusion Body Myositis|Sporadic Inclusion Body Myositis
University of Kansas Medical Center
 August 20 2020 Phase 1
NCT02246218 Completed
Urea Cycle Disorder
Horizon Therapeutics LLC|Horizon Pharma Ireland Ltd. Dublin Ireland
 December 31 2014 Phase 4
NCT01096095 Withdrawn
Spinocerebellar Ataxia Type 3
Hospital de Clinicas de Porto Alegre|Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul Brazil
 June 2010 Phase 2

Chemical Information & Solubility

Molecular Weight 187.19 Formula

C10H11O2.Na
CAS No. 1716-12-7 SDF Download 4-PBA (Sodium Phenylbutyrate) SDF
Smiles C1=CC=C(C=C1)CCCC(=O)[O-].[Na+]
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 37 mg/mL ( (197.66 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 37 mg/mL

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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