Home PI3K/Akt/mTOR Akt inhibitor SC66 For research use only.

SC66

SC66 is an allosteric inhibitor which displays a dual-inhibitory function toward AKT activity with IC50 values of 0.77, 2.85 and 0.47 μg/ml in HepG2, Huh7 and Hep3B cells after 72 h treatment, respectively.

SC66 Chemical Structure

SC66 Chemical Structure

CAS No. 871361-88-5

Purity & Quality Control

Batch: S531301 DMSO]55 mg/mL]false]]]false]]]false Purity: 99.97%
99.97

SC66 Related Products

Signaling Pathway

Akt Signaling Pathways

Akt Signaling Pathway

Biological Activity

Description SC66 is an allosteric inhibitor which displays a dual-inhibitory function toward AKT activity with IC50 values of 0.77, 2.85 and 0.47 μg/ml in HepG2, Huh7 and Hep3B cells after 72 h treatment, respectively.
Targets
Akt [1]
In vitro
In vitro SC66 reduces cell viability in a dose- and time-dependent manner, inhibits colony formation and induces apoptosis in HCC cells. SC66 treatment leads to a reduction in total and phospho-AKT levels. This is associated with alterations in cytoskeleton organization, a reduction in expression levels of E-cadherin, β-catenin and phospho-FAK, together with up-regulation of Snail protein levels. In addition, SC66 induces the production of reactive oxygen species (ROS) and DNA damage. SC66 affects AKT/mTOR signaling in HCC cell lines[1].
Cell Research Cell lines The human hepatocarcinoma cell lines HepG2, Huh7, PLC/PRF/5, Hep3B and HA22T/VGH cells
Concentrations 2 and 4 μg/ml
Incubation Time 1, 3 and 6 hours
Method Caspase activity assays: Cells (2 × 104/well) are treated with 2 and 4 μg/ml SC66 and after 1, 3 and 6 hours the levels of caspase3/7 activities in the cells are measured by the Caspase-Glo® 3/7 Assay. Results are expressed as relative luminescence units (RLU). Values are the mean ± SD of two separate experiments, each performed in duplicate.
In Vivo
In vivo In the mouse xenograft tumor model of Hep3B cells, SC66 treatment significantly reduces tumor volume to 37% on day 17 of treatment when compared with tumors in the untreated group. The inhibition of cell growth correlates with a reduction in phospho-AKT levels in the tumors of animals treated with SC66[1].
Animal Research Animal Models Male nude athymic mice (Fox1 nu/nu) aged 4 weeks inoculated with Hep3B cells at the right flank
Dosages 5 and 25 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 276.33 Formula

C18H16N2O
CAS No. 871361-88-5 SDF --
Smiles C1CC(=CC2=CC=NC=C2)C(=O)C(=CC3=CC=NC=C3)C1
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 55 mg/mL ( (199.03 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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