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Technical Data
| Formula | C18H16N2O |
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| Molecular Weight | 276.33 | CAS No. | 871361-88-5 | |
| Solubility (25°C)* | In vitro | DMSO | 55 mg/mL (199.03 mM) | |
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
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Biological Activity
| Description | SC66 is an allosteric inhibitor which displays a dual-inhibitory function toward AKT activity with IC50 values of 0.77, 2.85 and 0.47 μg/ml in HepG2, Huh7 and Hep3B cells after 72 h treatment, respectively. | |
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| In vitro | SC66 reduces cell viability in a dose- and time-dependent manner, inhibits colony formation and induces apoptosis in HCC cells. SC66 treatment leads to a reduction in total and phospho-AKT levels. This is associated with alterations in cytoskeleton organization, a reduction in expression levels of E-cadherin, β-catenin and phospho-FAK, together with up-regulation of Snail protein levels. In addition, SC66 induces the production of reactive oxygen species (ROS) and DNA damage. SC66 affects AKT/mTOR signaling in HCC cell lines[1]. | |
| In vivo | In the mouse xenograft tumor model of Hep3B cells, SC66 treatment significantly reduces tumor volume to 37% on day 17 of treatment when compared with tumors in the untreated group. The inhibition of cell growth correlates with a reduction in phospho-AKT levels in the tumors of animals treated with SC66[1]. |
Protocol (from reference)
| Cell Assay:[1] |
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| Animal Study:[1] |
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Selleck's SC66 has been cited by 6 publications
| Inhibition of autophagy can promote the apoptosis of bladder cancer cells induced by SC66 through the endoplasmic reticulum stress pathway [ Chem Biol Interact, 2023, 10.1016/j.cbi.2023.110725] | PubMed: 37741534 |
| SC66 inhibits the proliferation and induces apoptosis of human bladder cancer cells by targeting the AKT/β-catenin pathway [ J Cell Mol Med, 2021, 10.1111/jcmm.17005] | PubMed: 34687144 |
| [ Oncol Lett, 2020, ] | PubMed: 32863924 |
| Targeting Akt by SC66 triggers GSK-3β mediated apoptosis in colon cancer therapy. [ Cancer Cell Int, 2019, 19:124] | PubMed: 31168297 |
| Allosteric AKT Inhibitors Target Synthetic Lethal Vulnerabilities in E-Cadherin-Deficient Cells. [ Cancers (Basel), 2019, 11(9)] | PubMed: 31540244 |
| Transformation of normal cells by aberrant activation of YAP via cMyc with TEAD. [ Sci Rep, 2019, 9(1):10933] | PubMed: 31358774 |
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.