Home PI3K/Akt/mTOR Akt inhibitor - Bacterial chemical Oridonin For research use only.

Oridonin

Synonyms: NSC-250682, Isodonol, Rubescenin

Oridonin, a diterpenoid purified from Rabdosia rubescens, is a traditional agent with antitumor, anti-bacterial and anti-inflammatory effects. Oridonin inhibits AKT1 and AKT2 kinase activity with IC50 of 8.4 μM and 8.9 μM, respectively.

Oridonin Chemical Structure

Oridonin Chemical Structure

CAS No. 28957-04-2

Purity & Quality Control

Oridonin Related Products

Signaling Pathway

Akt Signaling Pathways

Akt Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HL60 Cytotoxicity assay 48 hrs Cytotoxicity against human HL60 cells after 48 hrs by MTT assay, IC50=2.5μM 23819871
RAW264.7 Antiinflammatory assay 18 hrs Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess method, IC50=5.2μM 23819871
K562 Antiproliferative assay 72 hrs Antiproliferative activity against human K562 cells after 72 hrs by MTT assay, IC50=4.33μM 28165738
Bel7402 Antiproliferative assay 72 hrs Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay, IC50=5.41μM 28165738
MGC803 Antiproliferative assay 72 hrs Antiproliferative activity against human MGC803 cells after 72 hrs by MTT assay, IC50=9.06μM 28165738
KB/CP4 Antiproliferative assay 48 hrs Antiproliferative activity against human KB/CP4 cells after 48 hrs by MTT assay, IC50=10.8μM 28165738
KB-3-1 Antiproliferative assay 48 hrs Antiproliferative activity against human KB-3-1 cells after 48 hrs by MTT assay, IC50=13.24μM 28165738
BEL7404/CP20 Antiproliferative assay 48 hrs Antiproliferative activity against human BEL7404/CP20 cells after 48 hrs by MTT assay, IC50=17.17μM 28165738
MCF7 Antiproliferative assay 72 hrs Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay, IC50=17.89μM 28165738
Bel7404 Antiproliferative assay 48 hrs Antiproliferative activity against human Bel7404 cells after 48 hrs by MTT assay, IC50=17.92μM 28165738
NCI-H460 Antiproliferative assay 48 hrs Antiproliferative activity against human NCI-H460 cells after 48 hrs by MTT assay, IC50=20.51μM 28165738
NCI-H460/MX20 Antiproliferative assay 48 hrs Antiproliferative activity against human NCI-H460/MX20 cells after 48 hrs by MTT assay, IC50=25.86μM 28165738
K562 Antiproliferative assay 72 hrs Antiproliferative activity against human K562 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=4.64μM 30921757
SGC7901 Antiproliferative assay 72 hrs Antiproliferative activity against human SGC7901 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=7.13μM 30921757
Bel7402 Antiproliferative assay 72 hrs Antiproliferative activity against human Bel7402 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=7.85μM 30921757
HL60 Antiproliferative assay 72 hrs Antiproliferative activity against human HL60 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=9.46μM 30921757
PC3 Antiproliferative assay 72 hrs Antiproliferative activity against human PC3 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=10.07μM 30921757
A549 Antiproliferative assay 72 hrs Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=18.15μM 30921757
L02 Cytotoxicity assay 72 hrs Cytotoxicity against human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=19.53μM 30921757
HCT116 Antiproliferative assay 48 hrs Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay, IC50=6.84μM 30981113
L02 Cytotoxicity assay 48 hrs Cytotoxicity in human L02 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay, IC50=6.97μM 30981113
Bel7402 Antiproliferative assay 48 hrs Antiproliferative activity against human Bel7402 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay, IC50=9.59μM 30981113
MCF7 Antiproliferative assay 48 hrs Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay, IC50=17.56μM 30981113
MDA-MB-231 Antiproliferative assay 48 hrs Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by MTT assay, EC50=29.4μM 30981113
HCT116 Cell cycle arrest assay 3 uM 24 hrs Induction in cell cycle arrest in human HCT116 cells assessed as inhibition in cyclin A protein expression level at 3 uM incubated for 24 hrs by Western blot analysis 30981113
HCT116 Cell cycle arrest assay 3 uM 24 hrs Induction in cell cycle arrest in human HCT116 cells assessed as inhibition in cyclin B1 protein expression level at 3 uM incubated for 24 hrs by Western blot analysis 30981113
HCT116 Apoptotic assay 1 uM 24 hrs Induction of apoptotic morphological change in human HCT116 cells assessed as loss of original morphology at 1 uM incubated for 24 hrs by haematoxylin-eosin staining based microscopic method 30981113
HCT116 Apoptotic assay 1 uM 24 hrs Induction of apoptotic morphological change in human HCT116 cells assessed as formation of unstructured state at 1 uM incubated for 24 hrs by haematoxylin-eosin staining based microscopic method 30981113
HCT116 Apoptotic assay 1 uM 24 hrs Induction of apoptotic morphological change in human HCT116 cells assessed as formation of volume swelling at 1 uM incubated for 24 hrs by haematoxylin-eosin staining based microscopic method 30981113
HCT116 Apoptotic assay 1 uM 24 hrs Induction of apoptotic morphological change in human HCT116 cells assessed as formation of condensed nuclei at 1 uM incubated for 24 hrs by haematoxylin-eosin staining based microscopic method 30981113
HCT116 Apoptotic assay 1 uM 24 hrs Induction of apoptotic morphological change in human HCT116 cells assessed as cell lysis at 1 uM incubated for 24 hrs by haematoxylin-eosin staining based microscopic method 30981113
HCT116 Apoptotic assay 1 uM 24 hrs Induction of apoptotic morphological change in human HCT116 cells assessed as membrane shrinkage at 1 uM incubated for 24 hrs by haematoxylin-eosin staining based microscopic method 30981113
PLC/PRF/5 Antiproliferative assay 72 hrs Antiproliferative activity against human PLC/PRF/5 cells incubated for 72 hrs by CCK8 cells, IC50=7.41μM 31200238
HepG2 Antiproliferative assay 72 hrs Antiproliferative activity against human HepG2 cells incubated for 72 hrs by CCK8 cells, IC50=8.12μM 31200238
RPMI8226 Antiproliferative assay 72 hrs Antiproliferative activity against human RPMI8226 cells incubated for 72 hrs by CCK8 cells, IC50=9.25μM 31200238
LO2 Cytotoxicity assay 72 hrs Cytotoxicity against human LO2 cells incubated for 72 hrs by CCK8 cells, IC50=17.47μM 31200238
A549 Antiproliferative assay 72 hrs Antiproliferative activity against human A549 cells incubated for 72 hrs by CCK8 cells, IC50=30.4μM 31200238
HepG2 Function assay 0.1 to 1 uM 10 days Inhibition of colony formation in human HepG2 cells at 0.1 to 1 uM incubated for 10 days by crystal violet staining based microscopic method 31200238
PLC/PRF/5 Function assay 0.001 to 1 uM 10 days Inhibition of colony formation in human PLC/PRF/5 cells assessed as decrease in colony size at 0.001 to 1 uM incubated for 10 days by crystal violet staining based microscopic method 31200238
K562 Antiproliferative assay 72 hrs Antiproliferative activity against human K562 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=4.79μM 31202992
SGC7901 Antiproliferative assay 72 hrs Antiproliferative activity against human SGC7901 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=7.87μM 31202992
Bel7402 Antiproliferative assay 72 hrs Antiproliferative activity against human Bel7402 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=8.31μM 31202992
A549 Antiproliferative assay 72 hrs Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=17.8μM 31202992
L02 Cytotoxicity assay 72 hrs Cytotoxicity against human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50=18.68μM 31202992
TE1 Antiproliferative assay 72 hrs Antiproliferative activity against human TE1 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay, IC50=12.73μM 31494472
A549 Antiproliferative assay 72 hrs Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay, IC50=13.45μM 31494472
MGC803 Antiproliferative assay 72 hrs Antiproliferative activity against human MGC803 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay, IC50=14.13μM 31494472
MCF7 Antiproliferative assay 72 hrs Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay, IC50=21.97μM 31494472
Click to View More Cell Line Experimental Data

Biological Activity

Description Oridonin, a diterpenoid purified from Rabdosia rubescens, is a traditional agent with antitumor, anti-bacterial and anti-inflammatory effects. Oridonin inhibits AKT1 and AKT2 kinase activity with IC50 of 8.4 μM and 8.9 μM, respectively.
Targets
Akt1 [5]
(Cell-free assay)		 Akt2 [5]
(Cell-free assay)
8.4 μM 8.9 μM
In vitro
In vitro

Oridonin effectively inhibites the proliferation of a wide variety of cancer cells including those from prostate (LNCaP, DU145, PC3), breast (MCF-7, MDA-MB231), non-small cell lung (NSCL) (NCI-H520, NCI-H460, NCI-H1299) cancers, acute promyelocytic leukemia (NB4), and glioblastoma multiforme (U118, U138) with ED50s ranging from 1.8 to 7.5 μg/ml. [1] The antitumor activities of Oridonin may due to its inhibitory effects on NF-kappaB and MAPKs signal pathways. [2] [3]
Experimental Result Images Methods Biomarkers Images PMID
Western blot BCR-ABL / Myc p-AMPK / AMPK / Glut1 / LC3 28128329
Immunofluorescence HSF1 p65 28128329
Growth inhibition assay Cell viability 26496199
In Vivo
In vivo

LD50: Mice 35-40mg/kg. [4]

Chemical Information & Solubility

Molecular Weight 364.43 Formula

C20H28O6
CAS No. 28957-04-2 SDF Download Oridonin SDF
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 72 mg/mL ( (197.56 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 34 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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