Home PI3K/Akt/mTOR Akt inhibitor - Apoptosis related activator Akti-1/2 For research use only.

Akti-1/2

Synonyms: Akt Inhibitor VIII

Akti-1/2 (Akt Inhibitor VIII) is a highly selective Akt1/Akt2 inhibitor with IC50 of 58 nM/210 nM, respectively, about 36-fold selectivity for Akt1 over Akt3. Akti-1/2 induces apoptosis.

Akti-1/2 Chemical Structure

Akti-1/2 Chemical Structure

CAS No. 612847-09-3

Purity & Quality Control

Akti-1/2 Related Products

Signaling Pathway

Akt Signaling Pathways

Akt Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
NCI-H1563 cell Growth inhibition assay Inhibition of human NCI-H1563 cell growth in a cell viability assay, IC50=0.54246 μM SANGER
NCI-H1618 cel Growth inhibition assay Inhibition of human NCI-H1618 cell growth in a cell viability assay, IC50=21.9982 μM SANGER
NCI-H1623 cell Growth inhibition assay Inhibition of human NCI-H1623 cell growth in a cell viability assay, IC50=15.0596 μM SANGER
NCI-H1648 cell Growth inhibition assay Inhibition of human NCI-H1648 cell growth in a cell viability assay, IC50=5.7551 μM SANGER
NCI-H1650 cell Growth inhibition assay Inhibition of human NCI-H1650 cell growth in a cell viability assay, IC50=11.5134 μM SANGER
NCI-H1651 cell Growth inhibition assay Inhibition of human NCI-H1651 cell growth in a cell viability assay, IC50=16.4338 μM SANGER
NCI-H1703 cell Growth inhibition assay Inhibition of human NCI-H1703 cell growth in a cell viability assay, IC50=15.577 μM SANGER
NCI-H1734 cell Growth inhibition assay Inhibition of human NCI-H1734 cell growth in a cell viability assay, IC50=10.7788 μM SANGER
NCI-H1755 cell Growth inhibition assay Inhibition of human NCI-H1755 cell growth in a cell viability assay, IC50=1.05531 μM SANGER
NCI-H1770 cell Growth inhibition assay Inhibition of human NCI-H1770 cell growth in a cell viability assay, IC50=34.1793 μM SANGER
NCI-H1792 cell Growth inhibition assay Inhibition of human NCI-H1792 cell growth in a cell viability assay, IC50=29.8379 μM SANGER
NCI-H1963 cell Growth inhibition assay Inhibition of human NCI-H1963 cell growth in a cell viability assay, IC50=19.7494 μM SANGER
NCI-H1993 cell Growth inhibition assay Inhibition of human NCI-H1993 cell growth in a cell viability assay, IC50=22.615 μM SANGER
NCI-H2009 cell Growth inhibition assay Inhibition of human NCI-H2009 cell growth in a cell viability assay, IC50=7.39019 μM SANGER
NCI-H2030 cell Growth inhibition assay Inhibition of human NCI-H2030 cell growth in a cell viability assay, IC50=1.94483 μM SANGER
NCI-H2122 cell Growth inhibition assay Inhibition of human NCI-H2122 cell growth in a cell viability assay, IC50=10.1385 μM SANGER
NCI-H2126 cell Growth inhibition assay Inhibition of human NCI-H2126 cell growth in a cell viability assay, IC50=12.3371 μM SANGER
NCI-H2170 cell Growth inhibition assay Inhibition of human NCI-H2170 cell growth in a cell viability assay, IC50=18.1028 μM SANGER
NCI-H2196 cell Growth inhibition assay Inhibition of human NCI-H2196 cell growth in a cell viability assay, IC50=24.8003 μM SANGER
NCI-H2228 cell Growth inhibition assay Inhibition of human NCI-H2228 cell growth in a cell viability assay, IC50=2.28604 μM SANGER
NCI-H2342 cell Growth inhibition assay Inhibition of human NCI-H2342 cell growth in a cell viability assay, IC50=0.93779 μM SANGER
NCI-H2347 cell Growth inhibition assay Inhibition of human NCI-H2347 cell growth in a cell viability assay, IC50=16.1581 μM SANGER
NCI-H2405 cell Growth inhibition assay Inhibition of human NCI-H2405 cell growth in a cell viability assay, IC50=10.4212 μM SANGER
NCI-H2452 cell Growth inhibition assay Inhibition of human NCI-H2452 cell growth in a cell viability assay, IC50=26.6594 μM SANGER
NCI-H292 cell Growth inhibition assay Inhibition of human NCI-H292 cell growth in a cell viability assay, IC50=6.04837 μM SANGER
NCI-H345 cell Growth inhibition assay Inhibition of human NCI-H345 cell growth in a cell viability assay, IC50=16.2706 μM SANGER
NCI-H358 cell Growth inhibition assay Inhibition of human NCI-H358 cell growth in a cell viability assay, IC50=19.7233 μM SANGER
NCI-H460 cell Growth inhibition assay Inhibition of human NCI-H460 cell growth in a cell viability assay, IC50=4.62993 μM SANGER
NCI-H520 cell Growth inhibition assay Inhibition of human NCI-H520 cell growth in a cell viability assay, IC50=1.13303 μM SANGER
NCI-H522 cell Growth inhibition assay Inhibition of human NCI-H522 cell growth in a cell viability assay, IC50=11.0874 μM SANGER
NCI-H526 cell Growth inhibition assay Inhibition of human NCI-H526 cell growth in a cell viability assay, IC50=22.8873μM SANGER
NCI-H596 Growth inhibition assay Inhibition of human NCI-H596 cell growth in a cell viability assay, IC50=17.5708 μM SANGER
NCI-H630 cell Growth inhibition assay Inhibition of human NCI-H630 cell growth in a cell viability assay, IC50=5.51055 μM SANGER
NCI-H64 cell Growth inhibition assay Inhibition of human NCI-H64 cell growth in a cell viability assay, IC50=12.5431 μM SANGER
NCI-H650 cell Growth inhibition assay Inhibition of human NCI-H650 cell growth in a cell viability assay, IC50=7.59877 μM SANGER
NCI-H661 cell Growth inhibition assay Inhibition of human NCI-H661 cell growth in a cell viability assay, IC50=5.42326 μM SANGER
NCI-H747 cell Growth inhibition assay Inhibition of human NCI-H747 cell growth in a cell viability assay, IC50=7.62799 μM SANGER
NCI-H838 cell Growth inhibition assay Inhibition of human NCI-H838 cell growth in a cell viability assay, IC50=31.4118 μM SANGER
NCI-N87 cell Growth inhibition assay Inhibition of human NCI-N87 cell growth in a cell viability assay, IC50=29.3042 μM SANGER
NH-12 cell Growth inhibition assay Inhibition of human NH-12 cell growth in a cell viability assay, IC50=0.60864 μM SANGER
NKM-1 cell Growth inhibition assay Inhibition of human NKM-1 cell growth in a cell viability assay, IC50=2.75923 μM SANGER
NMC-G1 cell Growth inhibition assay Inhibition of human NMC-G1 cell growth in a cell viability assay, IC50=42.6693 μM SANGER
NOS-1 cell Growth inhibition assay Inhibition of human NOS-1 cell growth in a cell viability assay, IC50=1.36953 μM SANGER
NUGC-3 cell Growth inhibition assay Inhibition of human NUGC-3 cell growth in a cell viability assay, IC50=16.5906 μM SANGER
NY cell Growth inhibition assay Inhibition of human NY cell growth in a cell viability assay, IC50=31.1864 μM SANGER
OAW-42 cell Growth inhibition assay Inhibition of human OAW-42 cell growth in a cell viability assay, IC50=7.31565 μM SANGER
OCI-AML2 cell Growth inhibition assay Inhibition of human OCI-AML2 cell growth in a cell viability assay, IC50=33.2531 μM SANGER
OCUB-M cell Growth inhibition assay Inhibition of human OCUB-M cell growth in a cell viability assay, IC50=7.72152 μM SANGER
OE19 cell Growth inhibition assay Inhibition of human OE19 cell growth in a cell viability assay, IC50=7.76684 μM SANGER
OE33 cell Growth inhibition assay Inhibition of human OE33 cell growth in a cell viability assay, IC50=1.88926 μM SANGER
OMC-1 cell Growth inhibition assay Inhibition of human OMC-1 cell growth in a cell viability assay, IC50=9.25098 μM SANGER
OS-RC-2 cell Growth inhibition assay Inhibition of human OS-RC-2 cell growth in a cell viability assay, IC50=8.52307 μM SANGER
OVCAR-3 cell Growth inhibition assay Inhibition of human OVCAR-3 cell growth in a cell viability assay, IC50=1.84415 μM SANGER
OVCAR-4 cell Growth inhibition assay Inhibition of human OVCAR-4 cell growth in a cell viability assay, IC50=30.466 μM SANGER
P12-ICHIKAWA cell Growth inhibition assay Inhibition of human P12-ICHIKAWA cell growth in a cell viability assay, IC50=14.7683 μM SANGER
P30-OHK cell Growth inhibition assay Inhibition of human P30-OHK cell growth in a cell viability assay, IC50=4.536 μM SANGER
PA-1 cell Growth inhibition assay Inhibition of human PA-1 cell growth in a cell viability assay, IC50=9.07615 μM SANGER
PANC-03-27 cell Growth inhibition assay Inhibition of human PANC-03-27 cell growth in a cell viability assay, IC50=8.71082 μM SANGER
PANC-10-05 cell Growth inhibition assay Inhibition of human PANC-10-05 cell growth in a cell viability assay, IC50=14.9959 μM SANGER
PC-3 cell Growth inhibition assay Inhibition of human PC-3 cell growth in a cell viability assay, IC50=10.5135 μM SANGER
PF-382 cell Growth inhibition assay Inhibition of human PF-382 cell growth in a cell viability assay, IC50=11.6828 μM SANGER
RCC10RGB cell Growth inhibition assay Inhibition of human RCC10RGB cell growth in a cell viability assay, IC50=8.6567 μM SANGER
RD cell Growth inhibition assay Inhibition of human RD cell growth in a cell viability assay, IC50=20.6036 μM SANGER
REH cell Growth inhibition assay Inhibition of human REH cell growth in a cell viability assay, IC50=11.4883 μM SANGER
RERF-LC-MS cell Growth inhibition assay Inhibition of human RERF-LC-MS cell growth in a cell viability assay, IC50=26.1347 μM SANGER
RKO cell Growth inhibition assay Inhibition of human RKO cell growth in a cell viability assay, IC50=19.4439 μM SANGER
RO82-W-1 cell Growth inhibition assay Inhibition of human RO82-W-1 cell growth in a cell viability assay, IC50=47.2783 μM SANGER
RPMI-2650 cell Growth inhibition assay Inhibition of human RPMI-2650 cell growth in a cell viability assay, IC50=5.77947 μM SANGER
RPMI-8402 cell Growth inhibition assay Inhibition of human RPMI-8402 cell growth in a cell viability assay, IC50=16.343 μM SANGER
RS4-11 cell Growth inhibition assay Inhibition of human RS4-11 cell growth in a cell viability assay, IC50=6.68645 μM SANGER
RT-112 cell Growth inhibition assay Inhibition of human RT-112 cell growth in a cell viability assay, IC50=6.71952 μM SANGER
RVH-421 cell Growth inhibition assay Inhibition of human RVH-421 cell growth in a cell viability assay, IC50=32.3606 μM SANGER
RXF393 cell Growth inhibition assay Inhibition of human RXF393 cell growth in a cell viability assay, IC50=20.5863 μM SANGER
SBC-5 cell Growth inhibition assay Inhibition of human SBC-5 cell growth in a cell viability assay, IC50=20.8325 μM SANGER
SCC-25 cell Growth inhibition assay Inhibition of human SCC-25 cell growth in a cell viability assay, IC50=15.8179 μM SANGER
SCC-4 cell Growth inhibition assay Inhibition of human SCC-4 cell growth in a cell viability assay, IC50=9.59283 μM SANGER
SCC-9 cell Growth inhibition assay Inhibition of human SCC-9 cell growth in a cell viability assay, IC50=3.08536 μM SANGER
SF126 cell Growth inhibition assay Inhibition of human SF126 cell growth in a cell viability assay, IC50=10.6746 μM SANGER
SF268 cell Growth inhibition assay Inhibition of human SF268 cell growth in a cell viability assay, IC50=15.1387 μM SANGER
SF295 cell Growth inhibition assay Inhibition of human SF295 cell growth in a cell viability assay, IC50=13.6183 μM SANGER
SIG-M5 cell Growth inhibition assay Inhibition of human SIG-M5 cell growth in a cell viability assay, IC50=12.6604 μM SANGER
SJSA-1 cell Growth inhibition assay Inhibition of human SJSA-1 cell growth in a cell viability assay, IC50=36.9072 μM SANGER
SK-CO-1 cell Growth inhibition assay Inhibition of human SK-CO-1 cell growth in a cell viability assay, IC50=10.6394 μM SANGER
SK-LMS-1 cell Growth inhibition assay Inhibition of human SK-LMS-1 cell growth in a cell viability assay, IC50=8.76452 μM SANGER
SK-LU-1 cell Growth inhibition assay Inhibition of human SK-LU-1 cell growth in a cell viability assay, IC50=42.043 μM SANGER
SK-MEL-24 cell Growth inhibition assay Inhibition of human SK-MEL-24 cell growth in a cell viability assay, IC50=13.1675 μM SANGER
SK-MEL-3 cell Growth inhibition assay Inhibition of human SK-MEL-3 cell growth in a cell viability assay, IC50=21.4696 μM SANGER
SK-MES-1 cell Growth inhibition assay Inhibition of human SK-MES-1 cell growth in a cell viability assay, IC50=2.10096 μM SANGER
human SN12C cell Growth inhibition assay Inhibition of human SN12C cell growth in a cell viability assay, IC50=17.1967 μM SANGER
SNB75 cell Growth inhibition assay Inhibition of human SNB75 cell growth in a cell viability assay, IC50=7.8297 μM SANGER
SNU-387 cell Growth inhibition assay Inhibition of human SNU-387 cell growth in a cell viability assay, IC50=4.19544 μM SANGER
SNU-423 cell Growth inhibition assay Inhibition of human SNU-423 cell growth in a cell viability assay, IC50=5.94933 μM SANGER
SNU-C1 cell Growth inhibition assay Inhibition of human SNU-C1 cell growth in a cell viability assay, IC50=2.00948 μM SANGER
SNU-C2B cell Growth inhibition assay Inhibition of human SNU-C2B cell growth in a cell viability assay, IC50=22.2673 μM SANGER
SR cell Growth inhibition assay Inhibition of human SR cell growth in a cell viability assay, IC50=13.0746 μM SANGER
SU-DHL-1 cell Growth inhibition assay Inhibition of human SU-DHL-1 cell growth in a cell viability assay, IC50=6.81543 μM SANGER
SW1710 cell Growth inhibition assay Inhibition of human SW1710 cell growth in a cell viability assay, IC50=1.38892 μM SANGER
SW780 cell Growth inhibition assay Inhibition of human SW780 cell growth in a cell viability assay, IC50=4.89358 μM SANGER
SW872 cell Growth inhibition assay Inhibition of human SW872 cell growth in a cell viability assay, IC50=14.1435 μM SANGER
SW954 cell Growth inhibition assay Inhibition of human SW954 cell growth in a cell viability assay, IC50=5.85783 μM SANGER
T47D cell Growth inhibition assay Inhibition of human T47D cell growth in a cell viability assay, IC50=0.5162 μM SANGER
22RV1 cell Growth inhibition assay Inhibition of human 22RV1 cell growth in a cell viability assay, IC50=6.61424 μM SANGER
23132-87 cell Growth inhibition assay Inhibition of human 23132-87 cell growth in a cell viability assay, IC50=0.75938 μM SANGER
639-V cell Growth inhibition assay Inhibition of human 639-V cell growth in a cell viability assay, IC50=5.8773 μM SANGER
697 cell Growth inhibition assay Inhibition of human 697 cell growth in a cell viability assay, IC50=7.17746 μM SANGER
769-P cell Growth inhibition assay Inhibition of human 769-P cell growth in a cell viability assay, IC50=1.10483 μM SANGER
8-MG-BA cell Growth inhibition assay Inhibition of human 8-MG-BA cell growth in a cell viability assay, IC50=8.63401 μM SANGER
A4-Fuk cell Growth inhibition assay Inhibition of human A4-Fuk cell growth in a cell viability assay, IC50=1.8583 μM SANGER
A427 cell Growth inhibition assay Inhibition of human A427 cell growth in a cell viability assay, IC50=3.07693 μM SANGER
A498 cell Growth inhibition assay Inhibition of human A498 cell growth in a cell viability assay, IC50=1.08134 μM SANGER
AN3-CA cell Growth inhibition assay Inhibition of human AN3-CA cell growth in a cell viability assay, IC50=1.65611 μM SANGER
AU565 cell Growth inhibition assay Inhibition of human AU565 cell growth in a cell viability assay, IC50=1.23917 μM SANGER
BB30-HNC cell Growth inhibition assay Inhibition of human BB30-HNC cell growth in a cell viability assay, IC50=2.86449 μM SANGER
BxPC-3 cell Growth inhibition assay Inhibition of human BxPC-3 cell growth in a cell viability assay, IC50=2.22442 μM SANGER
C-33-A cell Growth inhibition assay Inhibition of human C-33-A cell growth in a cell viability assay, IC50=12.4783 μM SANGER
C2BBe1 cell Growth inhibition assay Inhibition of human C2BBe1 cell growth in a cell viability assay, IC50=13.6679 μM SANGER
C3A cell Growth inhibition assay Inhibition of human C3A cell growth in a cell viability assay, IC50=4.42192 μM SANGER
CAL-33 cell Growth inhibition assay Inhibition of human CAL-33 cell growth in a cell viability assay, IC50=3.43125 μM SANGER
CAL-51 cell Growth inhibition assay Inhibition of human CAL-51 cell growth in a cell viability assay, IC50=7.62841 Μm SANGER
CAL-54 cell Growth inhibition assay Inhibition of human CAL-54 cell growth in a cell viability assay, IC50=4.88625 μM SANGER
CAL-85-1 cell Growth inhibition assay Inhibition of human CAL-85-1 cell growth in a cell viability assay, IC50=5.95309 μM SANGER
TE-10 cell Growth inhibition assay Inhibition of human TE-10 cell growth in a cell viability assay, IC50=9.04225 μM SANGER
TE-15 cell Growth inhibition assay Inhibition of human TE-15 cell growth in a cell viability assay, IC50=6.49393 μM SANGER
TE-5 cell Growth inhibition assay Inhibition of human TE-5 cell growth in a cell viability assay, IC50=4.82452 μM SANGER
TE-8 cell Growth inhibition assay Inhibition of human TE-8 cell growth in a cell viability assay, IC50=24.5243 μM SANGER
TT cell Growth inhibition assay Inhibition of human TT cell growth in a cell viability assay, IC50=5.32724 μM SANGER
U-118-MG cell Growth inhibition assay Inhibition of human U-118-MG cell growth in a cell viability assay, IC50=9.90258 μM SANGER
U-698-M cell Growth inhibition assay Inhibition of human U-698-M cell growth in a cell viability assay, IC50=39.0015 μM SANGER
U251 cell Growth inhibition assay Inhibition of human U251 cell growth in a cell viability assay, IC50=19.204 μM SANGER
UACC-62 cell Growth inhibition assay Inhibition of human UACC-62 cell growth in a cell viability assay, IC50=27.0545 μM SANGER
UACC-893 cell Growth inhibition assay Inhibition of human UACC-893 cell growth in a cell viability assay, IC50=5.29339 μM SANGER
VMRC-RCZ cell Growth inhibition assay Inhibition of human VMRC-RCZ cell growth in a cell viability assay, IC50=3.70955 μM SANGER
YKG-1 cell Growth inhibition assay Inhibition of human YKG-1 cell growth in a cell viability assay, IC50=1.01806 μM SANGER
CCF-STTG1 cell Growth inhibition assay Inhibition of human CCF-STTG1 cell growth in a cell viability assay, IC50=6.81501 μM SANGER
CCRF-CEM cell Growth inhibition assay Inhibition of human CCRF-CEM cell growth in a cell viability assay, IC50=4.93541 μM SANGER
CESS cell Growth inhibition assay Inhibition of human CESS cell growth in a cell viability assay, IC50=2.70089 μM SANGER
CHP-212 cell Growth inhibition assay Inhibition of human CHP-212 cell growth in a cell viability assay, IC50=4.55867 μM SANGER
COLO-205 cell Growth inhibition assay Inhibition of human COLO-205 cell growth in a cell viability assay, IC50=1.99706 μM SANGER
COR-L279 cell Growth inhibition assay Inhibition of human COR-L279 cell growth in a cell viability assay, IC50=1.68064 μM SANGER
CTB-1 cell Growth inhibition assay Inhibition of human CTB-1 cell growth in a cell viability assay, IC50=7.59751 μM SANGER
Ca-Ski cell Growth inhibition assay Inhibition of human Ca-Ski cell growth in a cell viability assay, IC50=5.3411 μM SANGER
Ca9-22 cell Growth inhibition assay Inhibition of human Ca9-22 cell growth in a cell viability assay, IC50=5.97333 μM SANGER
Calu-1 cell Growth inhibition assay Inhibition of human Calu-1 cell growth in a cell viability assay, IC50=7.25613 μM SANGER
Calu-3 cell Growth inhibition assay Inhibition of human Calu-3 cell growth in a cell viability assay, IC50=3.65321 μM SANGER
DMS-273 cell Growth inhibition assay Inhibition of human DMS-273 cell growth in a cell viability assay, IC50=4.15134 μM SANGER
DOK cell Growth inhibition assay Inhibition of human DOK cell growth in a cell viability assay, IC50=4.15134 μM SANGER
DSH1 cell Growth inhibition assay Inhibition of human DSH1 cell growth in a cell viability assay, IC50=0.98722 μM SANGER
DV-90 cell Growth inhibition assay Inhibition of human DV-90 cell growth in a cell viability assay, IC50=6.03122 μM SANGER
Detroit562 cell Growth inhibition assay Inhibition of human Detroit562 cell growth in a cell viability assay, IC50=4.79465 μM SANGER
DoTc2-4510 cell Growth inhibition assay Inhibition of human DoTc2-4510 cell growth in a cell viability assay, IC50=3.43202 μM SANGER
EB2 cell Growth inhibition assay Inhibition of human EB2 cell growth in a cell viability assay, IC50=7.75468 μM SANGER
ECC12 cell Growth inhibition assay Inhibition of human ECC12 cell growth in a cell viability assay, IC50=8.88569 μM SANGER
EFM-19 cell Growth inhibition assay Inhibition of human EFM-19 cell growth in a cell viability assay, IC50=0.56408 μM SANGER
EFO-21 cell Growth inhibition assay Inhibition of human EFO-21 cell growth in a cell viability assay, IC50=4.43468 μM SANGER
ES7 cell Growth inhibition assay Inhibition of human ES7 cell growth in a cell viability assay, IC50=1.34511 μM SANGER
ETK-1 Growth inhibition assay Inhibition of human ETK-1 cell growth in a cell viability assay, IC50=4.57383 μM SANGER
EW-3 cell Growth inhibition assay Inhibition of human EW-3 cell growth in a cell viability assay, IC50=5.64414 μM SANGER
HCC70 cell Growth inhibition assay Inhibition of human HCC70 cell growth in a cell viability assay, IC50=0.75078 μM SANGER
HGC-27 cell Growth inhibition assay Inhibition of human HGC-27 cell growth in a cell viability assay, IC50=0.15033 μM SANGER
IGROV-1 cell Growth inhibition assay Inhibition of human IGROV-1 cell growth in a cell viability assay, IC50=0.72328 Μm SANGER
MDA-MB-361 cell Growth inhibition assay Inhibition of human MDA-MB-361 cell growth in a cell viability assay, IC50=0.48995 μM SANGER
MDA-MB-415 cell Growth inhibition assay Inhibition of human MDA-MB-415 cell growth in a cell viability assay, IC50=1.73449 μM SANGER
MDA-MB-468 cell Growth inhibition assay Inhibition of human MDA-MB-468 cell growth in a cell viability assay, IC50=3.70773 μM SANGER
ME-180 cell Growth inhibition assay Inhibition of human ME-180 cell growth in a cell viability assay, IC50=3.15011 Μm SANGER
NCI-H526 cells Growth inhibition assay Inhibition of Akt-mediated antiapoptotic activity in human NCI-H526 cells assessed as disruption of protein-Apaf-1 complex at 50 uM after 24 hrs by Western blot analysis 17603540
Click to View More Cell Line Experimental Data

Biological Activity

Description Akti-1/2 (Akt Inhibitor VIII) is a highly selective Akt1/Akt2 inhibitor with IC50 of 58 nM/210 nM, respectively, about 36-fold selectivity for Akt1 over Akt3. Akti-1/2 induces apoptosis.
Targets
Akt1 [1]
(Cell-free assay)		 Akt2 [1]
(Cell-free assay)		 Akt3 [1]
(Cell-free assay)
58 nM 210 nM 2119 nM
In vitro
In vitro

In a cell-based IPKA (C33A) assay, Akti-1/2 inhibits Akt1 and Akt2 with IC50 of 305 nM and 2086 nM, respectively. In HT29, MCF7, and A2780 cells, Akti-1/2 induces cell apoptosis by dramatically increasing caspase-3 activity. [1]

In liver cells, Akti-1/2 blocks regulation of PEPCK, G6Pase expression, and FOXO1 activity. [2]

Akti-1/2 also strongly potentiates PAR-1-mediated platelet aggregation by blocking PKB. [3]

In HCC827, NCI-H522, NCI-1651, and PC-9 cells, Akti-1/2 inhibits cell growth with IC50 of 4.7 μM, 7.25 μM, and 9.5 μM, when in combination, Akti-1/2 causes enhanced inhibition of cell growth and apoptosis. [4]
Kinase Assay Kinase screen
Briefly, all assays (25.5 μl at 21°C for 30 min) are performed using a Biomek 2000 Laboratory Automation Workstation in a 96-well format. Reactions contains 5–20 mU purified kinase along with substrate peptide or protein and are initiated by the addition of 10 mM MgAcetate and 5, 20, or 50 μM ATP ([γ-33P]-ATP, 800 cpm/pmol).
Cell Research Cell lines HCC827, NCI-H522, NCI-1651, and PC-9 cells
Concentrations ~10 μM
Incubation Time 96 h
Method

The cell growth inhibitory effect of AKTi-1/2 is studied using the 96 h sulforhodamine B assay (SRB). Drug concentrations that inhibited 50% of cell growth (IC50) are calculated for each compound in GraphPad Prism 6.0 using non-linear regression analysis and sigmoidal dose–response (variable slope) equation.
Experimental Result Images Methods Biomarkers Images PMID
Western blot p-AKT / AKT / p-Foxo3a / p-GSK3β / p-p70S6K / p-S6 / p-4EBP1 / Cyclin D1 / Cyclin D2 / Cyclin D3 / p-Rb / p27 18725974
Immunofluorescence MCM2 / H3K9me2 / CD63 26637368
In Vivo
In vivo

In mice, Akti-1/2 (50 mg/kg, i.p.) inhibits basal and IGF-stimulated Akt1 and Akt2 phosphorylation in lung. [1]
Animal Research Animal Models C57BL/6 J mice
Dosages 50 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 551.64 Formula

C34H29N7O
CAS No. 612847-09-3 SDF Download Akti-1/2 SDF
Smiles C1CN(CCC1N2C3=CC=CC=C3NC2=O)CC4=CC=C(C=C4)C5=C(N=C6C=C7C(=NC=N7)C=C6N5)C8=CC=CC=C8
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 22 mg/mL ( (39.88 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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