Home Endocrinology & Hormones Estrogen/progestogen Receptor agonist Psoralidin For research use only.

Psoralidin

Psoralidin, a naturally occurring coumestan isolated from the fractions of organic solvents such as ethylacetate, hexane, or n-butanol of the seed extract of Psoralea corylifolia L., has a variety of biological activities such as anticancer, antioxidant, antibacterial, antidepressant, anti-inflammatory activities, and regulation of insulin signaling. It is an agonist for both estrogen receptor (ER)α and ERβ with binding affinities (IC50s) of 1.03 and 24.6 μM, respectively.

Psoralidin Chemical Structure

Psoralidin Chemical Structure

CAS No. 18642-23-4

Purity & Quality Control

Batch: S546401 DMSO]67 mg/mL]false]Ethanol]1 mg/mL]false]Water]Insoluble]false Purity: 99.96%
99.96

Psoralidin Related Products

Signaling Pathway

Estrogen/progestogen Receptor Signaling Pathways

Estrogen/progestogen Receptor Signaling Pathway

Biological Activity

Description Psoralidin, a naturally occurring coumestan isolated from the fractions of organic solvents such as ethylacetate, hexane, or n-butanol of the seed extract of Psoralea corylifolia L., has a variety of biological activities such as anticancer, antioxidant, antibacterial, antidepressant, anti-inflammatory activities, and regulation of insulin signaling. It is an agonist for both estrogen receptor (ER)α and ERβ with binding affinities (IC50s) of 1.03 and 24.6 μM, respectively.
Targets
ERα [1]
(Cell-free)		 ERβ [1]
(Cell-free)
1.03 μM 24.6 μM
In vitro
In vitro Psoralidin has been characterized as a full ER agonist, which activates the classical ER-signaling pathway in both ER-positive human breast and endometrial cell lines as well as non-human cultured cells transiently expressing either ERα or ERβ. Psoralidin was also able to induce the endogenous estrogen-responsive gene, pS2, in human breast cancer cells MCF-7. The IC50 values for psoralidin to replace the binding of E2 to both receptors were 1.03 and 24.6 μM, respectively. Psoralidin is able to bind to both receptors within the micromolar range and has preferential affinity for ERα over ERβ[1].
Cell Research Cell lines MCF-7 cells
Concentrations 10 μM
Incubation Time 24 h
Method

MCF-7 cells were cultured in estrogen-free RPMI 1640 media for 4 days before transfection, and plated (4 × 105 cells/well) in triplicate onto a 12-well plate. Cells were transiently transfected with pERE-luciferase plasmid (0.5 μg/well) and with an internal control plasmid pRL-Tk (0.1 μg/well) using Lipofectamine 2000 Reagent. ERE-luciferase plasmid contains three copies of the Xenopus laevis vitellogenin A2 ERE upstream of fire fly luciferase. The pRL-Tk plasmid contains a cDNA encoding Renilla luciferase. At 24 h post-transfection, cells were treated DMSO, various concentrations of E2, psoralidin, ICI, or appropriate combination of two compounds and incubated for another 24 h. The cells were harvested with Passive Lysis buffer. Luciferase activity present in the cell lysates was measured.
In Vivo
In vivo Acute and subchronic administrations of psoralidin produced a significant reduction in immobility time in the mouse forced swimming test. Psoralidin dose-dependently increased swimming and failed to affect climbing[2].
Animal Research Animal Models Male ICR strain of mice
Dosages 20, 40 and 60 mg/kg
Administration oral

Chemical Information & Solubility

Molecular Weight 336.34 Formula

C20H16O5
CAS No. 18642-23-4 SDF --
Smiles CC(=CCC1=CC2=C(C=C1O)OC(=O)C3=C2OC4=C3C=CC(=C4)O)C
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 67 mg/mL ( (199.2 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 1 mg/mL

Water : Insoluble


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