Home Endocrinology & Hormones Estrogen/progestogen Receptor agonist Equol For research use only.

Equol

Equol ,a metabolite of soybeans, is an important isoflavone in humans，specifically binds to 5α-DHT, has a modest affinity for recombinant estrogen receptor ERβ. Phase 2.

Equol Chemical Structure

Equol Chemical Structure

CAS No. 531-95-3

Purity & Quality Control

Equol Related Products

Signaling Pathway

Estrogen/progestogen Receptor Signaling Pathways

Estrogen/progestogen Receptor Signaling Pathway

Biological Activity

Description Equol ,a metabolite of soybeans, is an important isoflavone in humans，specifically binds to 5α-DHT, has a modest affinity for recombinant estrogen receptor ERβ. Phase 2.
Features A chiral molecule that can exist as the enantiomers R-equol and S-equol.
Targets
ERβ [1]
In vitro
In vitro Equol is a metabolite produced from the soy phytoestrogen daidzein by the action of gut microflora. Equol has higher affinity for both ERs (estrogen receptors including ERalpha and ERbeta). Equol exists as the enantiomers R-equol and S-equol. S-equol has a high binding affinity, preferential for ERbeta with a Ki of 16 nM whereas R-equol binds more weakly and with a preference for ERalpha K with a Ki of 50 nM. [1] Equol is superior to all other isoflavones in its antioxidant activity. [2] Equol has antioestrogenic properties. [3] Equol is a 100-fold more potent than daidzein in stimulating an oestrogenic response. Equol is also more effective than daidzein in competing with 3 H-oestradiol for binding to the ER. Equol stimulates the growth of MCF-7 cells in a concentration-dependent manner. Although equol exhibits oestrogenic activity, exposure of MCF-7 cells to equol simultaneously with oestradiol is effective in reducing pS2 mRNA expression. Equol results in the downregulation of ER mRNA expression. [4]
Cell Research Cell lines MCF-7 cells
Concentrations 0 μM-10 μM
Incubation Time 24 hours
Method Each well of a 24-well plate is seeded with 1 × 105 MCF-7 cells in 1 mL of media B. Twenty-four hours after plating, equol at the indicated concentration is added. Equol is dissolved in ethanol (final concentration of ethanol in the medium is 1%). The medium is changed every 24 hours and equol is replenished with each change. Cell growth is determined on the sixth day by the sulphorhodamine colorimetric assay. After colour development, aliquots are pipetted into a 96-well microtitre plate and the absorbance is determined at 570 nm using an Elisa microplate reader.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06133101 Recruiting
Non-Alcoholic Fatty Liver Disease
Marialena Mouzaki|Children''s Hospital Medical Center Cincinnati
 January 15 2024 Phase 2
NCT06398236 Recruiting
Menopause
Nutrition & Sante Iberia|Adknoma Health Research
 March 17 2023 Not Applicable
NCT01982578 Completed
Alzheimer''s Disease
Fundación para la Investigación del Hospital Clínico de Valencia|University of Valencia
 September 1 2017 Not Applicable
NCT03101085 Completed
Alzheimer Disease
Russell Swerdlow|Ausio Pharmaceuticals LLC|University of Kansas Medical Center
 May 5 2017 Phase 1|Phase 2

Chemical Information & Solubility

Molecular Weight 242.27 Formula

C15H14O3
CAS No. 531-95-3 SDF Download Equol SDF
Smiles C1C(COC2=C1C=CC(=C2)O)C3=CC=C(C=C3)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 48 mg/mL ( (198.12 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 48 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Equol | Equol supplier | purchase Equol | Equol cost | Equol manufacturer | order Equol | Equol distributor