Home Endocrinology & Hormones Estrogen/progestogen Receptor modulator Pregnenolone For research use only.

Pregnenolone

Synonyms: 3β-Hydroxy-5-pregnen-20-one

Pregnenolone(3β-Hydroxy-5-pregnen-20-one) is an endogenous steroid hormone, used in the treatment of fatigue, Alzheimer’s disease, trauma and injuries.

Pregnenolone Chemical Structure

Pregnenolone Chemical Structure

CAS No. 145-13-1

Purity & Quality Control

Pregnenolone Related Products

Signaling Pathway

Estrogen/progestogen Receptor Signaling Pathways

Estrogen/progestogen Receptor Signaling Pathway

Biological Activity

Description Pregnenolone(3β-Hydroxy-5-pregnen-20-one) is an endogenous steroid hormone, used in the treatment of fatigue, Alzheimer’s disease, trauma and injuries.
Targets
progestogen Receptor [1]
In vitro
In vitro

Pregnenolone induces a large, dose-related increase of both the rate and extent of MAP2-induced tubulin assembly, whereas progesterone, inactive per se, counteracted the stimulatory effect of Pregnenolone. Pregnenolone-increased assembly of microtubules produces a completely normal structure. [1] Pregnenolone preserves microtubule abundance and promotes cell movement during epiboly. [2] Pregnenolone results in dramatic reduction in GR nuclear localization in mouse hippocampal cell line (HT-22). Pregnenolone has neuroprotective effects against both glutamate and amyloid beta protein neuropathology. [3]
In Vivo
In vivo

Pregnenolone sustains its proliferative activity in vivo and stimulates the growth of LNCaP-tumor xenografts in intact male SCID mice as well as in castrated animals. Pregnenolone is shown to activate transcription through the LNCaP androgen receptor (AR), but not the wild-type AR. [4] Pregnenolone results in a significant decrease in the accumulation of astrocytes in the proximity of the wound and in a decreased bromodeoxyuridine incorporation in reactive astrocytes of rats. [5] Pregnenolone administration results in elevations in downstream neurosteroids such as allopregnanolone, a molecule with neuroprotective effects that also increases neurogenesis, decreases apoptosis and inflammation, modulates the hypothalamic-pituitary-adrenal axis, and markedly increases GABA(A) receptor responses. Pregnenolone administration elevates pregnenolone sulfate, a neurosteroid that positively modulates NMDA receptors. [6]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05781009 Recruiting
Alcohol Use Disorder
Yale University|National Institute on Alcohol Abuse and Alcoholism (NIAAA)
 January 8 2024 Phase 2
NCT05903716 Recruiting
Acne Vulgaris
Marmara University
 September 15 2022 --
NCT04791124 Unknown status
Down Syndrome
Parc de Salut Mar|Aelis Farma|Starlab
 January 11 2021 --
NCT03801629 Completed
Drug Effect
Yale University
 September 12 2019 Phase 1
NCT03953612 Completed
Cocaine-Related Disorders
Yale University|National Institute on Drug Abuse (NIDA)
 March 12 2019 Early Phase 1

Chemical Information & Solubility

Molecular Weight 316.48 Formula

C21H32O2
CAS No. 145-13-1 SDF Download Pregnenolone SDF
Smiles CC(=O)C1CCC2C1(CCC3C2CC=C4C3(CCC(C4)O)C)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 22 mg/mL ( (69.51 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 22 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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