Psoralidin

Catalog No.S5464 Batch:S546401

Technical Data

Formula	C₂₀H₁₆O₅
Molecular Weight	336.34	CAS No.	18642-23-4
Solubility (25°C)*	In vitro	DMSO	67 mg/mL (199.2 mM)
		Ethanol	1 mg/mL (2.97 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Psoralidin, a naturally occurring coumestan isolated from the fractions of organic solvents such as ethylacetate, hexane, or n-butanol of the seed extract of Psoralea corylifolia L., has a variety of biological activities such as anticancer, antioxidant, antibacterial, antidepressant, anti-inflammatory activities, and regulation of insulin signaling. It is an agonist for both estrogen receptor (ER)α and ERβ with binding affinities (IC50s) of 1.03 and 24.6 μM, respectively.

Targets

ERα ^[1] (Cell-free)	ERβ ^[1] (Cell-free)
1.03 μM	24.6 μM

In vitro

Psoralidin has been characterized as a full ER agonist, which activates the classical ER-signaling pathway in both ER-positive human breast and endometrial cell lines as well as non-human cultured cells transiently expressing either ERα or ERβ. Psoralidin was also able to induce the endogenous estrogen-responsive gene, pS2, in human breast cancer cells MCF-7. The IC50 values for psoralidin to replace the binding of E2 to both receptors were 1.03 and 24.6 μM, respectively. Psoralidin is able to bind to both receptors within the micromolar range and has preferential affinity for ERα over ERβ^[1].

In vivo

Acute and subchronic administrations of psoralidin produced a significant reduction in immobility time in the mouse forced swimming test. Psoralidin dose-dependently increased swimming and failed to affect climbing^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines MCF-7 cells Concentrations 10 μM Incubation Time 24 h Method MCF-7 cells were cultured in estrogen-free RPMI 1640 media for 4 days before transfection, and plated (4 × 10⁵ cells/well) in triplicate onto a 12-well plate. Cells were transiently transfected with pERE-luciferase plasmid (0.5 μg/well) and with an internal control plasmid pRL-Tk (0.1 μg/well) using Lipofectamine 2000 Reagent. ERE-luciferase plasmid contains three copies of the Xenopus laevis vitellogenin A2 ERE upstream of fire fly luciferase. The pRL-Tk plasmid contains a cDNA encoding Renilla luciferase. At 24 h post-transfection, cells were treated DMSO, various concentrations of E2, psoralidin, ICI, or appropriate combination of two compounds and incubated for another 24 h. The cells were harvested with Passive Lysis buffer. Luciferase activity present in the cell lysates was measured.
Animal Study:^{^[2]}	Animal Models Male ICR strain of mice Dosages 20, 40 and 60 mg/kg Administration oral

Cell Assay:^{^[1]}

Cell lines
MCF-7 cells
Concentrations
10 μM
Incubation Time
24 h
Method
MCF-7 cells were cultured in estrogen-free RPMI 1640 media for 4 days before transfection, and plated (4 × 10⁵ cells/well) in triplicate onto a 12-well plate. Cells were transiently transfected with pERE-luciferase plasmid (0.5 μg/well) and with an internal control plasmid pRL-Tk (0.1 μg/well) using Lipofectamine 2000 Reagent. ERE-luciferase plasmid contains three copies of the Xenopus laevis vitellogenin A2 ERE upstream of fire fly luciferase. The pRL-Tk plasmid contains a cDNA encoding Renilla luciferase. At 24 h post-transfection, cells were treated DMSO, various concentrations of E2, psoralidin, ICI, or appropriate combination of two compounds and incubated for another 24 h. The cells were harvested with Passive Lysis buffer. Luciferase activity present in the cell lysates was measured.

Animal Study:^{^[2]}

Animal Models
Male ICR strain of mice
Dosages
20, 40 and 60 mg/kg
Administration
oral

References

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.