Home Apoptosis Apoptosis related chemical Protocatechuic acid For research use only.

Protocatechuic acid

Synonyms: 3,4-Dihydroxybenzoic acid, Protocatechuate

Protocatechuic acid (PCA, 3,4-Dihydroxybenzoic acid, Protocatechuate), a dihydroxybenzoic acid, is a type of widely distributed naturally occurring phenolic acid.

Protocatechuic acid Chemical Structure

Protocatechuic acid Chemical Structure

CAS No. 99-50-3

Purity & Quality Control

Batch: S397501 DMSO]30 mg/mL]false]]]false]]]false Purity: 99.99%
99.99

Protocatechuic acid Related Products

Biological Activity

Description Protocatechuic acid (PCA, 3,4-Dihydroxybenzoic acid, Protocatechuate), a dihydroxybenzoic acid, is a type of widely distributed naturally occurring phenolic acid.
In vitro
In vitro Protocatechuic acid (PCA) inhibits cell migration and invasion at non-cytotoxic concentrations. It down-regulates the Ras/Akt/NF-κB pathway by targeting RhoB activation, which in turn leads to a reduction of MMP-mediated cellular events in cancer cells. In some cancer cells, PCA treatment suppresses cell adhesion and the production of interleukin (IL)-6, IL-8, vascular endothelial growth factor and the intercellular adhesion molecule-1, which would further attenuate angiogenic and metastatic actions[1]. PCA will lower the expression of cleaved caspase-3 and decrease the rate of cardiomyocyte apoptosis induced by hypoxia/reoxygenation. PCA is proven to have the neuroprotective effects in PC12 cells[2].
Cell Research Cell lines human gastric adenocarcinoma (AGS) cells
Concentrations 1 mM
Incubation Time 24, 48 and 72 h
Method

Cells (1 × 104 cells/mL) are seeded in 12 well plates and treated with PCA (1.0 mM) or dimethyl sulphoxide (DMSO) as control for 24, 48 and 72 h. The number of cells is measured by the Trypan blue dye exclusion assay.
In Vivo
In vivo Protocatechuic acid (PCA) inhibits metastasis of B16/F10 melanoma cells to the liver in mice[1]. PCA is seen to enhance learning and memory ability, and alleviate oxidative stress, apoptosis and glial proliferation following CIH exposure in rats. In addition, PCA administration also decreases the level of IL-1β in brain and increases the expression of BDNF and SYN. Its administration inhibits apoptosis by decreasing JNK/P38 phosphoryltion and the downstream target including c-fos, Bax and Cleaved Caspase-3 in the hippocampal and prefrontal cortex areas. PCA exerts significant neuroprotective effects in CIH rats[2].
Animal Research Animal Models 6-week-old C57/BL6 mice with B16/F10 melanoma
Dosages 200 and 400 mg/kg
Administration oral administration
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06396000 Not yet recruiting
Anesthesia; Adverse Effect
Saiqiang Guo|Tongji Hospital
 May 31 2024 --

Chemical Information & Solubility

Molecular Weight 154.12 Formula

C7H6O4
CAS No. 99-50-3 SDF Download Protocatechuic acid SDF
Smiles C1=CC(=C(C=C1C(=O)O)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 30 mg/mL ( (194.65 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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