Protocatechuic acid

Catalog No.S3975 Batch:S397501

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Technical Data

Formula

C7H6O4
Molecular Weight 154.12 CAS No. 99-50-3
Solubility (25°C)* In vitro DMSO 30 mg/mL (194.65 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Protocatechuic acid (PCA, 3,4-Dihydroxybenzoic acid, Protocatechuate), a dihydroxybenzoic acid, is a type of widely distributed naturally occurring phenolic acid.
In vitro Protocatechuic acid (PCA) inhibits cell migration and invasion at non-cytotoxic concentrations. It down-regulates the Ras/Akt/NF-κB pathway by targeting RhoB activation, which in turn leads to a reduction of MMP-mediated cellular events in cancer cells. In some cancer cells, PCA treatment suppresses cell adhesion and the production of interleukin (IL)-6, IL-8, vascular endothelial growth factor and the intercellular adhesion molecule-1, which would further attenuate angiogenic and metastatic actions[1]. PCA will lower the expression of cleaved caspase-3 and decrease the rate of cardiomyocyte apoptosis induced by hypoxia/reoxygenation. PCA is proven to have the neuroprotective effects in PC12 cells[2].
In vivo Protocatechuic acid (PCA) inhibits metastasis of B16/F10 melanoma cells to the liver in mice[1]. PCA is seen to enhance learning and memory ability, and alleviate oxidative stress, apoptosis and glial proliferation following CIH exposure in rats. In addition, PCA administration also decreases the level of IL-1β in brain and increases the expression of BDNF and SYN. Its administration inhibits apoptosis by decreasing JNK/P38 phosphoryltion and the downstream target including c-fos, Bax and Cleaved Caspase-3 in the hippocampal and prefrontal cortex areas. PCA exerts significant neuroprotective effects in CIH rats[2].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    human gastric adenocarcinoma (AGS) cells

  • Concentrations

    1 mM

  • Incubation Time

    24, 48 and 72 h

  • Method

    Cells (1 × 104 cells/mL) are seeded in 12 well plates and treated with PCA (1.0 mM) or dimethyl sulphoxide (DMSO) as control for 24, 48 and 72 h. The number of cells is measured by the Trypan blue dye exclusion assay.
Animal Study:

[1]
  • Animal Models

    6-week-old C57/BL6 mice with B16/F10 melanoma

  • Dosages

    200 and 400 mg/kg

  • Administration

    oral administration

Selleck's Protocatechuic acid has been cited by 1 publication

Cinnamic acid inhibits cell viability, invasion, and glycolysis in primary endometrial stromal cells by suppressing NF-κB-induced transcription of PKM2 [ Biosci Rep, 2021, BSR20211828] PubMed: 34499120

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.