Home Apoptosis Apoptosis related inhibitor Importazole For research use only.

Importazole

Importazole is a small molecule inhibitor of the transport receptor importin-β (Karyopherin beta) specifically blocking importin-β-mediated nuclear import. Importazole induces apoptosis and inhibits tumor growth.

Importazole Chemical Structure

Importazole Chemical Structure

CAS No. 662163-81-7

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Importazole Related Products

Biological Activity

Description Importazole is a small molecule inhibitor of the transport receptor importin-β (Karyopherin beta) specifically blocking importin-β-mediated nuclear import. Importazole induces apoptosis and inhibits tumor growth.
Targets
importin-β [1]
In vitro
In vitro Importazole is an effective inhibitor of the Ran/importin-β interaction in vitro. Importazole specifically blocks importin-β-mediated nuclear import both in Xenopus egg extracts and cultured cells, without disrupting transportin-mediated nuclear import or CRM1-mediated nuclear export. When added during mitosis, importazole impairs the release of an importin-β cargo FRET probe and causes both predicted and novel defects in spindle assembly[1]. It also inhibits proliferation and induces apoptosis of multiple myeloma cells by blocking the NF-κB signal pathway in vitro[2].
Cell Research Cell lines HEK 293 cells
Concentrations 40 μM
Incubation Time 1 h
Method For all import and export experiments, HEK 293 cells stably expressing NFAT-GFP are grown on glass coverslips to approximately 50% confluency prior to drug treatment. In all cases, importazole is used at 40 μM and leptomycin B is used at 10 ng/ml. For controls, DMSO is used at a concentration of 0.4%. Ionomycin is added at 1.25 μM. Importazole and leptomycin B treatments are all for 1 hour. In all experiments cells are fixed with 4% formaldehyde prior to fluorescence microscopy. DNA is visualized with 1 μg/ml Hoechst dye. For quantification, 100 cells from each condition are analyzed and the percentage that shows nuclear accumulation of NFAT-GFP calculated.
Experimental Result Images Methods Biomarkers Images PMID
Western blot Cyclin B1 / CDK1 / p-RCC1 / Cyclin D1 c-Myc 30742095

Chemical Information & Solubility

Molecular Weight 318.42 Formula

C20H22N4
CAS No. 662163-81-7 SDF Download Importazole SDF
Smiles CC(C1=CC=CC=C1)NC2=NC(=NC3=CC=CC=C32)N4CCCC4
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 63 mg/mL ( (197.85 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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