Home Apoptosis Apoptosis related chemical Crocin For research use only.

Crocin

Synonyms: Alpha-Crocin, Gardenia Yellow

Crocin (Alpha-Crocin, Gardenia Yellow) is a water-soluble carotenoid pigment of saffron (Crocus sativus L.). It has been used as a spice for flavoring and coloring food preparations, and in Chinese traditional medicine as an anodyne or tranquilizer.

Crocin Chemical Structure

Crocin Chemical Structure

CAS No. 42553-65-1

Purity & Quality Control

Crocin Related Products

Biological Activity

Description Crocin (Alpha-Crocin, Gardenia Yellow) is a water-soluble carotenoid pigment of saffron (Crocus sativus L.). It has been used as a spice for flavoring and coloring food preparations, and in Chinese traditional medicine as an anodyne or tranquilizer.
In vitro
In vitro Crocin can significantly inhibit the proliferation of MCF-7 cells, and induce their apoptosis through mitochondrial signaling pathways including the activation of Caspase-8, upregulation of Bax, the disruption of mitochondrial membrane potential (MMP), and the release of cytochrome c[1]. Crocin decreases cell viability in DLA (Daltons lymphoma) cells, in a concentration- and time-dependent manner[2].
Cell Research Cell lines MCF-7 cells
Concentrations 10, 25, 50 µg/ml
Incubation Time 24 h
Method

MCF-7 cells were treated with 10, 25, 50 µg/ml Crocin for 24 h and washed with PBS buffer, then fixed in ice-cold 70% ethanol for 15 min. A suspension of 100 µL was taken and incubated with 5 µL propidium iodine (20 µg/ml) and 5 µL annexin V-FITC in the dark for 30 min at room temperature. Finally, 400 µL cold PBS was added to each tube and analyzed by flow cytometry.
In Vivo
In vivo Crocin exhibits a variety of pharmacological effects in mice including inhibition of skin tumor growth, improvement of learning behavior previously impaired by ethanol, anti-hyperlipidemic effects, therapeutic efficacy for colon adenocarcinomas in rats, anti-atherosclerotic effects and anti-oxidant effects in PC-12 cells by increasing GSH synthesis. For Daltons lymphoma models, The lifespan of ascities tumor bearing mice treated with crocin, are found to be significantly increasing and a significant reduction in solid tumour volume is found in crocin treated animals when compared to that of control animals. Crocin also shows significant impact on hematological parameters, like the hemoglobin count and numbers of lymphocytes[2]. It has shown that crocin exhibits beneficial effects on many organs including the nervous system, the most studied, gastrointestinal, cardiovascular, genital, endocrine, immune systems, etc. At pharmacological doses it does not harm liver and other organs' function[3].
Animal Research Animal Models Swiss albino mice injected 1 × 106 Daltons lymphoma cells to the peritoneal cavity
Dosages 10 ml/kg
Administration oral

Chemical Information & Solubility

Molecular Weight 976.96 Formula

C44H64O24
CAS No. 42553-65-1 SDF Download Crocin SDF
Smiles CC(=CC=CC=C(C)C=CC=C(C)C(=O)OC1C(C(C(C(O1)COC2C(C(C(C(O2)CO)O)O)O)O)O)O)C=CC=C(C)C(=O)OC3C(C(C(C(O3)COC4C(C(C(C(O4)CO)O)O)O)O)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (102.35 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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