Home GPCR & G Protein Adrenergic Receptor inhibitor Propranolol HCl For research use only.

Propranolol HCl

Synonyms: AY-64043, ICI-45520, NCS-91523

Propranolol HCl (AY-64043, ICI-45520, NCS-91523) is a competitive non-selective beta-adrenergic receptors inhibitor.

Propranolol HCl Chemical Structure

Propranolol HCl Chemical Structure

CAS No. 318-98-9

Purity & Quality Control

Propranolol HCl Related Products

Signaling Pathway

Adrenergic Receptor Signaling Pathways

Adrenergic Receptor Signaling Pathway

Biological Activity

Description Propranolol HCl (AY-64043, ICI-45520, NCS-91523) is a competitive non-selective beta-adrenergic receptors inhibitor.
Targets
β-adrenergic receptor [1]
12 nM
In vitro
In vitro

Propranolol, a non-selective β1 and β2 adrenergic blocker, has no effect on the proliferation of neonatal mouse cardiac fibroblasts (NMCFs).[2]
Cell Research Cell lines neonatal mice cardiomyocytes (NMCMs)
Concentrations 10, 20, 40, 50, and 100 μM
Incubation Time 6, 12, 18, and 24 h
Method

In vitro, the neonatal mice cardiomyocytes (NMCMs) are treated with different drugs of different concentration, including isoproterenol (0, 1, 2.5, 5, 10, 20, and 50 μM); amiodarone (0, 1, 2.5, 5, 10, 20, and 50 μM); metoprolol (0, 10, 20, 30, 50, 100, 150, and 200 μM); propranolol (0, 10, 20, 40, 50, and 100 μM); lidocaine (0, 1, 2.5, 5, 10, 20, and 50 μM); verapamil(0, 1, 2.5, 5, 10, 20, and 50 μM);ivabradine (0, 1, 2, 3, 5, 10, and 20 μM). And the concentrations of drugs that promote CMs proliferation most significantly are chosen for subsequent experiments, including isoproterenol (10 μM), amiodarone (5 μM), metoprolol (20 μM), propranolol (20 μM), lidocaine (5 μM), verapamil (2.5 μM), ivabradine (3 μM) for NMCMs, NMCFs, and hPSC-CM.
Experimental Result Images Methods Biomarkers Images PMID
Growth inhibition assay Cell viability 27582542
Western blot Bax / Bcl-2 / Cyt C / Caspase-9 / Cleaved Caspase-9 / Caspase-3 / Cleaved Caspase-3 B-Raf / p-B-Raf / MEK / p-MEK / ERK / p-ERK / AKT / p-AKT 27582542
In Vivo
In vivo

Propranolol, an anti-arrhythmic drugs, consistently reduces the heart rates of adult mice by approximately 10%-20%, whereas isoproterenol has the opposite effect.[2]
Animal Research Animal Models 1-day-old neonatal mice; 12-week-old adult mice
Dosages 10 mg/kg/d
Administration s.c.; ALZET Osmotic Pumps
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06145074 Recruiting
Pancreatic Cancer|Surgery
Zealand University Hospital|Rigshospitalet Denmark
 March 20 2024 Phase 2
NCT05968690 Recruiting
Advanced Melanoma
Ryan Stephenson|Rutgers The State University of New Jersey
 September 11 2023 Phase 1
NCT05679193 Recruiting
Prostate Cancer
Oslo University Hospital
 January 2 2023 Phase 2

Chemical Information & Solubility

Molecular Weight 295.8 Formula

C16H21NO2.HCl
CAS No. 318-98-9 SDF Download Propranolol HCl SDF
Smiles CC(C)NCC(COC1=CC=CC2=CC=CC=C21)O.Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 59 mg/mL ( (199.45 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 6 mg/mL

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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