Propranolol HCl

Catalog No.S4076 Batch:S407603

Technical Data

Formula	C₁₆H₂₁NO₂.HCl
Molecular Weight	295.8	CAS No.	318-98-9
Solubility (25°C)*	In vitro	Water	100 mg/mL (338.06 mM)
		DMSO	59 mg/mL (199.45 mM)

* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Propranolol HCl (AY-64043, ICI-45520, NCS-91523) is a competitive non-selective beta-adrenergic receptors inhibitor.

Targets

β-adrenergic receptor ^[1]

12 nM

In vitro

Propranolol, a non-selective β1 and β2 adrenergic blocker, has no effect on the proliferation of neonatal mouse cardiac fibroblasts (NMCFs).^[2]

In vivo

Propranolol, an anti-arrhythmic drugs, consistently reduces the heart rates of adult mice by approximately 10%-20%, whereas isoproterenol has the opposite effect.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines neonatal mice cardiomyocytes (NMCMs) Concentrations 10, 20, 40, 50, and 100 μM Incubation Time 6, 12, 18, and 24 h Method In vitro, the neonatal mice cardiomyocytes (NMCMs) are treated with different drugs of different concentration, including isoproterenol (0, 1, 2.5, 5, 10, 20, and 50 μM); amiodarone (0, 1, 2.5, 5, 10, 20, and 50 μM); metoprolol (0, 10, 20, 30, 50, 100, 150, and 200 μM); propranolol (0, 10, 20, 40, 50, and 100 μM); lidocaine (0, 1, 2.5, 5, 10, 20, and 50 μM); verapamil(0, 1, 2.5, 5, 10, 20, and 50 μM);ivabradine (0, 1, 2, 3, 5, 10, and 20 μM). And the concentrations of drugs that promote CMs proliferation most significantly are chosen for subsequent experiments, including isoproterenol (10 μM), amiodarone (5 μM), metoprolol (20 μM), propranolol (20 μM), lidocaine (5 μM), verapamil (2.5 μM), ivabradine (3 μM) for NMCMs, NMCFs, and hPSC-CM.
Animal Study:^{^[2]}	Animal Models 1-day-old neonatal mice; 12-week-old adult mice Dosages 10 mg/kg/d Administration s.c.; ALZET Osmotic Pumps

Cell Assay:^{^[2]}

Cell lines
neonatal mice cardiomyocytes (NMCMs)
Concentrations
10, 20, 40, 50, and 100 μM
Incubation Time
6, 12, 18, and 24 h
Method
In vitro, the neonatal mice cardiomyocytes (NMCMs) are treated with different drugs of different concentration, including isoproterenol (0, 1, 2.5, 5, 10, 20, and 50 μM); amiodarone (0, 1, 2.5, 5, 10, 20, and 50 μM); metoprolol (0, 10, 20, 30, 50, 100, 150, and 200 μM); propranolol (0, 10, 20, 40, 50, and 100 μM); lidocaine (0, 1, 2.5, 5, 10, 20, and 50 μM); verapamil(0, 1, 2.5, 5, 10, 20, and 50 μM);ivabradine (0, 1, 2, 3, 5, 10, and 20 μM). And the concentrations of drugs that promote CMs proliferation most significantly are chosen for subsequent experiments, including isoproterenol (10 μM), amiodarone (5 μM), metoprolol (20 μM), propranolol (20 μM), lidocaine (5 μM), verapamil (2.5 μM), ivabradine (3 μM) for NMCMs, NMCFs, and hPSC-CM.

Animal Study:^{^[2]}

Animal Models
1-day-old neonatal mice; 12-week-old adult mice
Dosages
10 mg/kg/d
Administration
s.c.; ALZET Osmotic Pumps

References

Selleck's Propranolol HCl has been cited by 15 publications

Multiple cancer cell types release LIF and Gal3 to hijack neural signals [ Cell Res, 2024, 10.1038/s41422-024-00946-z]	PubMed: 38467743
Propafenone facilitates mitochondrial-associated ferroptosis and synergizes with immunotherapy in melanoma [ J Immunother Cancer, 2024, 12(11)e009805]	PubMed: 39581704
Stress-induced stenotic vascular remodeling via reduction of plasma omega-3 fatty acid metabolite 4-oxoDHA by noradrenaline [ Sci Rep, 2024, 14(1):4178]	PubMed: 38378892
Ketone bodies promote stroke recovery via GAT-1-dependent cortical network remodeling [ Cell Reports, 2023, 42(4)]	PubMed: None
Beta-blockers disrupt mitochondrial bioenergetics and increase radiotherapy efficacy independently of beta-adrenergic receptors in medulloblastoma [ EBioMedicine, 2022, 82:104149]	PubMed: 35816899
Moderate heart rate reduction promotes cardiac regeneration through stimulation of the metabolic pattern switch [ Cell Rep, 2022, 38(10):110468]	PubMed: 35263588
KCNQ1-deficient and KCNQ1-mutant human embryonic stem cell-derived cardiomyocytes for modeling QT prolongation [ Stem Cell Res Ther, 2022, 13(1):287]	PubMed: 35765105
Targeting the β2 -adrenergic receptor increases chemosensitivity in multiple myeloma by induction of apoptosis and modulating cancer cell metabolism [ J Pathol, 2022, 10.1002/path.6020]	PubMed: 36245401
Targeting ADRB2 enhances sensitivity of non-small cell lung cancer to VEGFR2 tyrosine kinase inhibitors [ Cell Death Discov, 2022, 8(1):36]	PubMed: 35075132
LncRNA MALAT1 Aggravates Renal Tubular Injury via Activating LIN28A and the Nox4/AMPK/mTOR Signaling Axis in Diabetic Nephropathy [ Front Endocrinol (Lausanne), 2022, 13:895360]	PubMed: 35813614

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