Home Endocrinology & Hormones Adrenergic Receptor antagonist Hydroxyflutamide (Hydroxyniphtholide) For research use only.

Hydroxyflutamide (Hydroxyniphtholide)

Synonyms: Hydroxy-flutamide, SCH-16423

Hydroxyflutamide (SCH-16423, Hydroxyniphtholide) is an androgen receptor (AR) antagonist and possesses an IC50 of 0.7 μM for the androgen receptor.

Hydroxyflutamide (Hydroxyniphtholide) Chemical Structure

Hydroxyflutamide (Hydroxyniphtholide) Chemical Structure

CAS No. 52806-53-8

Purity & Quality Control

Batch: S217401 DMSO]58 mg/mL]false]Ethanol]58 mg/mL]false]Water]Insoluble]false Purity: 99.93%
99.93

Hydroxyflutamide (Hydroxyniphtholide) Related Products

Signaling Pathway

Adrenergic Receptor Signaling Pathways

Adrenergic Receptor Signaling Pathway

Biological Activity

Description Hydroxyflutamide (SCH-16423, Hydroxyniphtholide) is an androgen receptor (AR) antagonist and possesses an IC50 of 0.7 μM for the androgen receptor.
Targets
AR [1]
0.7 μM
In vitro
In vitro

Hydroxyflutamide is an androgen receptor (AR) antagonist, which enhances the effects of irradiation in AR-positive cells cultured in the presence of androgens.[2]
Cell Research Cell lines Human urothelial cancer cell lines, UMUC3 and 5637
Concentrations 1 μM
Incubation Time 48 h
Method

Cells (2×105/dish) seeded in 35-mm tissue culture dishes and incubated in the presence or absence of DHT, HF, and/or inhibitors of DNA repair for 48 hours are irradiated using a Gammacell 40 Cs-137 irradiator (0.6 Gy/min). The irradiated cells are then used for MTT and clonogenic assays.
In Vivo
In vivo

In a mouse xenograft model, low-dose flutamide are found to enhance the inhibitory effects of irradiation, and its tumor size is similar to that of AR knockdown line with radiation alone.[2]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03348527 Completed
Prostate Cancer
Lidds AB|CMX Research
 May 12 2017 Phase 2
NCT02341404 Completed
Prostate Cancer
Lidds AB|Uppsala University
 May 2012 Phase 2

Chemical Information & Solubility

Molecular Weight 292.21 Formula

C11H11F3N2O4
CAS No. 52806-53-8 SDF Download Hydroxyflutamide (Hydroxyniphtholide) SDF
Smiles CC(C)(O)C(=O)NC1=CC=C(C(=C1)C(F)(F)F)[N+]([O-])=O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 58 mg/mL ( (198.48 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 58 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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