Hydroxyflutamide (Hydroxyniphtholide)

Catalog No.S2174 Batch:S217401

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Technical Data

Formula

C11H11F3N2O4
Molecular Weight 292.21 CAS No. 52806-53-8
Solubility (25°C)* In vitro DMSO 58 mg/mL (198.48 mM)
Ethanol 58 mg/mL (198.48 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Hydroxyflutamide (SCH-16423, Hydroxyniphtholide) is an androgen receptor (AR) antagonist and possesses an IC50 of 0.7 μM for the androgen receptor.
Targets
AR [1]
0.7 μM
In vitro

Hydroxyflutamide is an androgen receptor (AR) antagonist, which enhances the effects of irradiation in AR-positive cells cultured in the presence of androgens.[2]
In vivo

In a mouse xenograft model, low-dose flutamide are found to enhance the inhibitory effects of irradiation, and its tumor size is similar to that of AR knockdown line with radiation alone.[2]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    Human urothelial cancer cell lines, UMUC3 and 5637

  • Concentrations

    1 μM

  • Incubation Time

    48 h

  • Method

    Cells (2×105/dish) seeded in 35-mm tissue culture dishes and incubated in the presence or absence of DHT, HF, and/or inhibitors of DNA repair for 48 hours are irradiated using a Gammacell 40 Cs-137 irradiator (0.6 Gy/min). The irradiated cells are then used for MTT and clonogenic assays.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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