Home Transmembrane Transporters Calcium Channel inhibitor - Ras inhibitor Fendiline hydrochloride For research use only.

Fendiline hydrochloride

Fendiline hydrochloride is the hydrochloride salt form of Fendiline, which is an L-type calcium channel blocker and also a specific inhibitor of K-Ras plasma membrane targeting with no detectable effect on the localization of H- and N-Ras.

Fendiline hydrochloride Chemical Structure

Fendiline hydrochloride Chemical Structure

CAS No. 13636-18-5

Purity & Quality Control

Batch: S527901 DMSO]70 mg/mL]false]]]false]]]false Purity: 99.86%
99.86

Fendiline hydrochloride Related Products

Signaling Pathway

Calcium Channel Signaling Pathways

Calcium Channel Signaling Pathway

Biological Activity

Description Fendiline hydrochloride is the hydrochloride salt form of Fendiline, which is an L-type calcium channel blocker and also a specific inhibitor of K-Ras plasma membrane targeting with no detectable effect on the localization of H- and N-Ras.
Targets
K-ras [1] L-type calcium channel [1]
In vitro
In vitro Fendiline, an L-type calcium channel blocker, is a specific inhibitor of K-Ras plasma membrane targeting with no detectable effect on the localization of H- and N-Ras. Fendiline does not inhibit K-Ras posttranslational processing but significantly reduced nanoclustering of K-Ras and redistributed K-Ras from the plasma membrane to the endoplasmic reticulum (ER), Golgi apparatus, endosomes, and cytosol. Fendiline significantly inhibited signaling downstream of constitutively active K-Ras and endogenous K-Ras signaling in cells transformed by oncogenic H-Ras. Consistent with these effects, fendiline blocked the proliferation of pancreatic, colon, lung, and endometrial cancer cell lines expressing oncogenic mutant K-Ras[1].
Cell Research Cell lines MDCK cells
Concentrations --
Incubation Time 48 h
Method

MDCK cells stably expressing GFP-K-RasG12V were treated with 1% vehicle (DMSO) or various concentrations of fendiline for 48 h. Cells were trypsinized and resuspended in medium. Ten microliters of cell suspension was mixed with 10 μl of 0.4% trypan blue, and live/dead cell counts were obtained using Countess.

Chemical Information & Solubility

Molecular Weight 351.91 Formula

C23H25N.HCl
CAS No. 13636-18-5 SDF --
Smiles CC(C1=CC=CC=C1)NCCC(C2=CC=CC=C2)C3=CC=CC=C3.Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 70 mg/mL ( (198.91 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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