Home Autophagy Autophagy inhibitor - TLR inhibitor - COVID-19 inhibitor - HIV Protease inhibitor Chloroquine For research use only.

Chloroquine

Synonyms: NSC-187208, CHQ, CQ

Chloroquine is an antimalarial drug and autophagy/lysosome inhibitor. Chloroquine also suppresses Toll-like receptor-9 (TLR9) protein expression. Chloroquine is highly effective agianst SARS-CoV-2 (COVID-19) infection with EC50 of 1.13 μM in Vero E6 cells. Chloroquine has anti-HIV-1 activity.

Chloroquine Chemical Structure

Chloroquine Chemical Structure

CAS No. 54-05-7

Purity & Quality Control

Chloroquine Related Products

Signaling Pathway

Autophagy Signaling Pathways

Autophagy Signaling Pathway

Biological Activity

Description Chloroquine is an antimalarial drug and autophagy/lysosome inhibitor. Chloroquine also suppresses Toll-like receptor-9 (TLR9) protein expression. Chloroquine is highly effective agianst SARS-CoV-2 (COVID-19) infection with EC50 of 1.13 μM in Vero E6 cells. Chloroquine has anti-HIV-1 activity.
Targets
Autophagy [1] lysosome [1] TLR9 [2] HIV-1 [4] SARS-CoV-2 [3]
(Vero E6 cells based assay)
1.13 μM(EC50)
In vitro
In vitro

Chloroquine is a widely used autophagy inhibitor.[5]
Cell Research Cell lines HL‐60 cells
Concentrations 10 µM
Incubation Time 48 h
Method

One day before treatment, AML cells were seeded with a density of 2 × 105 cells/mL. Fresh medium containing different concentrations of Ara‐C was added into the culture medium. Cells were further cultured for 24 or 48 h based on experimental purposes. For inhibition of autophagy, HL‐60 cells were treated with 200 nM bafilomycin A1 or 10 µM chloroquine. Overexpression and knockdown were performed by transfection.

(Data sourced from selleck products)
In Vivo
In vivo

The inhibition of autophagy by chloroquine potentiates the therapeutic effect of PI3K/AKT inhibitors in vitro and in vivo TNBC models, synergizing with taxane-based chemotherapy.[6]
Animal Research Animal Models Female NOD/SCID athymic mice injected with MDAMB231 cells
Dosages 30 mg/kg
Administration o.g.

(Data sourced from selleck products)
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05972551 Recruiting
Osteogenesis Imperfecta
Amgen
 April 22 2024 Phase 3
NCT05890573 Recruiting
Pulmonary Fibrosis|COVID-19 Pneumonia
Second Affiliated Hospital School of Medicine Zhejiang University
 April 16 2024 Not Applicable
NCT05841758 Not yet recruiting
Sarcoidosis Pulmonary
Hospices Civils de Lyon
 April 1 2024 Phase 4
NCT03994874 Not yet recruiting
Congestive Heart Failure
Iperboreal Pharma Srl
 March 2024 Phase 1|Phase 2
NCT06117722 Not yet recruiting
Chronic Heart Failure
Elpen Pharmaceutical Co. Inc.
 March 1 2024 --

Chemical Information & Solubility

Molecular Weight 319.87 Formula

C18H26ClN3
CAS No. 54-05-7 SDF --
Smiles CCN(CC)CCCC(C)NC1=C2C=CC(=CC2=NC=C1)Cl
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 64 mg/mL ( (200.08 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 64 mg/mL

Water : Insoluble


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