Home Autophagy Autophagy activator QX77 For research use only.

QX77

QX77 is a novel activator of chaperone-mediated autophagy (CMA). QX77 induces the up-regulation of Rab11 expression and up-regulates LAMP2A expression.

QX77 Chemical Structure

QX77 Chemical Structure

CAS No. 1798331-92-6

Purity & Quality Control

Batch: S679701 DMSO]15 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.73%
99.73

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Biological Activity

Description QX77 is a novel activator of chaperone-mediated autophagy (CMA). QX77 induces the up-regulation of Rab11 expression and up-regulates LAMP2A expression.
Targets
CMA [1]
In vitro
In vitro

QX77, an activator of CMA, eliminates the effects of milk fat globule-EGF factor 8 (MFG-E8) on TGF-β1-induced human pancreatic stellate cells (PSCs) activation. QX77 mitigates the suppressive effect of MFG-E8 on oxidative stress in activated PSCs.[2]
Cell Research Cell lines Human pancreatic stellate cells
Concentrations 5 ng/ml
Incubation Time 24 h
Method

Human pancreatic stellate cells (1×106/well) are treated with 5 ng/ml TGF-β1 with or without 20 ng/ml MFG-E8 for 24 h. 5 ng/mL QX77 is added simultaneously with 20 ng/mL MFG-E8 in TGF-β1-treated HPSCs. 24 h later, the cells are collected for various measurements. Western blot analyse the expression of GRP78, p-PERK, PERK, LAMP2A and MEF2D in the HPSCs .

Chemical Information & Solubility

Molecular Weight 300.74 Formula

C16H13ClN2O2
CAS No. 1798331-92-6 SDF --
Smiles CC(=O)NC1=CC=C(C=C1)C2=NC3=C(C=C(C=C3)Cl)OC2
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 15 mg/mL ( (49.87 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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